Protein-Peptide Affinity Determination Using an H/D Exchange Dilution Strategy: Application to Antigen-Antibody Interactions

Journal of the American Society for Mass Spectrometry

Volumn 21, Issue 10, 2010, Pages 1660-1667

  Tu, Tingting ^a   Drǎguşanu, Mihaela ^b   Petre, Brînduşa Alina ^b   Rempel, Don L ^a   Przybylski, Michael ^b   Gross, Michael L ^a  

^a WASHINGTON UNIVERSITY   (United States)

^b UNIVERSITY OF KONSTANZ   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

3-NITROTYROSINE; ANALYTICAL TECHNIQUES; ANTIGEN-ANTIBODY INTERACTION; BINDING AFFINITIES; BINDING INTERFACE; DISSOCIATION CONSTANT; H/D EXCHANGE; MODEL SYSTEM; NANOMOLAR RANGE; NITROTYROSINE; PEPTIDE LIGAND; PROTEIN-LIGAND INTERACTIONS; PROTEIN-PEPTIDE INTERACTIONS; QUANTITATIVE DETERMINATIONS;

AMIDES; AMINO ACIDS; ANTIGENS; BINDING ENERGY; CALCIUM; DISSOCIATION; LIGANDS; MASS SPECTROMETRY; ORGANIC ACIDS; PEPTIDES; STOICHIOMETRY;

ANTIBODIES;

3 NITROTYROSINE; AMINO ACID; BETA ENDORPHIN; CALCIUM; CALMODULIN; IMMUNOGLOBULIN G; MONOCLONAL ANTIBODY; OPIATE PEPTIDE; PROSTACYCLIN SYNTHASE;

ANTIGEN ANTIBODY REACTION; ARTICLE; BINDING AFFINITY; CONTROLLED STUDY; DEUTERIUM HYDROGEN EXCHANGE; DILUTION; DISSOCIATION CONSTANT; IMMUNOASSAY; MASS SPECTROMETRY; PROTEIN BINDING; PROTEIN INTERACTION; PROTEIN MODIFICATION; QUANTITATIVE ANALYSIS; SCIENTIFIC LITERATURE; SENSITIVITY ANALYSIS; STOICHIOMETRY; TITRIMETRY;

ANIMALS; ANTIBODIES, MONOCLONAL; BETA-ENDORPHIN; CALMODULIN; DEUTERIUM EXCHANGE MEASUREMENT; KINETICS; MASS SPECTROMETRY; PROTEIN BINDING; PROTEIN INTERACTION MAPPING; SWINE;

EID: 77956881928     PISSN: 10440305     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jasms.2010.03.030     Document Type: Article

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