Does Chemical Cross-Linking with NHS Esters Reflect the Chemical Equilibrium of Protein-Protein Noncovalent Interactions in Solution?

Journal of the American Society for Mass Spectrometry

Volumn 21, Issue 10, 2010, Pages 1775-1783

  Mädler, Stefanie ^a   Seitz, Markus ^b   Robinson, John ^b   Zenobi, Renato ^a  

^a ETH ZURICH   (Switzerland)

^b UNIVERSITY OF ZURICH   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

BINDING AFFINITIES; CHEMICAL CROSS-LINKING; CHEMICAL EQUILIBRIUMS; CONCENTRATION DEPENDENCE; CROSS-LINKED COMPLEX; EXPERIMENTAL APPROACHES; GOOD CORRELATIONS; MALDI-MS; MATRIX-ASSISTED LASER DESORPTION/IONIZATION MASS SPECTROMETRY; NON-COVALENT INTERACTION; NONCOVALENT PROTEINS; PROTEIN COMPLEXES; TRYPSIN INHIBITOR;

BINDING ENERGY; COMPLEXATION; DESORPTION; ESTERIFICATION; ESTERS; MASS SPECTROMETRY; PEPTIDES; PHASE EQUILIBRIA;

CROSSLINKING;

BASIC PANCREATIC TRYPSIN INHIBITOR; N HYDROXYSUCCINIMIDE; STAT6 PROTEIN; STEROID RECEPTOR COACTIVATOR 1; SUBERIC ACID BIS(N HYDROXYSUCCINIMIDE) ESTER; THROMBIN; TRYPSIN INHIBITOR; UNCLASSIFIED DRUG;

ARTICLE; BINDING AFFINITY; COMPLEX FORMATION; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; CROSS LINKING; MASS SPECTROMETRY; MATRIX ASSISTED LASER DESORPTION IONIZATION MASS SPECTROMETRY; PROTEIN BINDING; PROTEIN PROTEIN INTERACTION;

AMINO ACID SEQUENCE; ANIMALS; CATTLE; CROSS-LINKING REAGENTS; HUMANS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; NUCLEAR RECEPTOR COACTIVATOR 1; PROTEIN BINDING; PROTEIN INTERACTION MAPPING; PROTEINS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; STAT6 TRANSCRIPTION FACTOR; SUCCINIMIDES;

EID: 77956881617     PISSN: 10440305     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jasms.2010.06.016     Document Type: Article

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