New method for counting bacteria associated with coral mucus

Applied and Environmental Microbiology

Volumn 76, Issue 18, 2010, Pages 6128-6133

  Garren, Melissa ^a   Azam, Farooq ^a  

^a UNIVERSITY OF CALIFORNIA SAN DIEGO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL CELLS; BIOVOLUMES; CELL SIZE; EPIFLUORESCENCE MICROSCOPY; IN-CELL; MICROBIAL ECOLOGY; MUCUS LAYER; PORITES LOBATA; TRYPSINIZATION; ZOOXANTHELLAE;

BACTERIOLOGY; BODY FLUIDS; CYTOLOGY; ECOLOGY; REEFS; SEAWATER; WASTEWATER;

CELLS;

4',6 DIAMIDINO 2 PHENYLINDOLE; INDOLE DERIVATIVE;

ABUNDANCE; BACTERIUM; CORAL REEF; CYTOLOGY; MICROSCOPY; SAMPLING; SEAWATER; WASTEWATER;

ANALYSIS OF VARIANCE; ANIMAL; ANTHOZOA; ARTICLE; BACTERIAL COUNT; BACTERIUM; DINOFLAGELLATE; FLUORESCENCE MICROSCOPY; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGY; MUCUS; SCANNING ELECTRON MICROSCOPY; ULTRASTRUCTURE; UNITED STATES;

ANALYSIS OF VARIANCE; ANIMALS; ANTHOZOA; BACTERIA; COLONY COUNT, MICROBIAL; DINOFLAGELLIDA; HAWAII; INDOLES; MICROSCOPY, ELECTRON, SCANNING; MICROSCOPY, FLUORESCENCE; MUCUS;

HAWAII [(ISL) HAWAIIAN ISLANDS]; HAWAII [UNITED STATES]; HAWAIIAN ISLANDS; UNITED STATES;

ANTHOZOA; BACTERIA (MICROORGANISMS); PORITES LOBATA;

EID: 77956851396     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.01100-10     Document Type: Article

References (34)

1. Ali, A., Q. Zhang, J. S. Dai, and X. Huang. 2003. Calcein as a fluorescent iron chemosensor for the determination of low molecular weight iron in biological fluids. Biometals 16:285-293. (Pubitemid 36135461)
2. Alldredge, A. L., U. Passow, and B. E. Logan. 1993. The abundance and significance of a class of large, transparent organic particles in the ocean. Deep Sea Res. A 40:1131-1140.
3. Allers, E., C. Niesner, C. Wild, and J. Pernthaler. 2008. Microbes enriched in seawater after addition of coral mucus. Appl. Environ. Microbiol. 74: 3274-3278.
4. Benson, A. A., and L. Muscatin. 1974. Wax in coral mucus-energy-transfer from corals to reef fishes. Limnol. Oceanogr. 19:810-814.
5. Bratbak, G. 1985. Bacterial biovolume and biomass estimations. Appl. Environ. Microbiol. 49:1488-1493.
6. Brown, B. E., and J. C. Bythell. 2005. Perspectives on mucus secretion in reef corals. Mar. Ecol. Prog. Ser. 296:291-309.
7. Coffroth, M. A. 1984. Ingestion and incorporation of coral mucus aggregates by a gorgonian soft coral. Mar. Ecol. Prog. Ser. 17:193-199.
8. Coffroth, M. A. 1990. Mucous sheet formation on poritid corals-an evaluation of coral mucus as a nutrient source on reefs. Mar. Biol. 105:39-49.
9. Coligan, J., B. Dunn, D. Speicher, and P. Wingfield (ed.). 2003. Short protocols in protein science. Wiley, New York, NY.
10. 15N values in algal tissue to map locations and potential sources of anthropogenic nutrient inputs on the island of Maui, Hawai'i, USA. Mar. Pollut. Bull. 60:655-671.
11. Ducklow, H. W., and R. Mitchell. 1979. Bacterial-populations and adaptations in the mucus layers on living corals. Limnol. Oceanogr. 24:715-725.
12. Ducklow, H. W., and R. Mitchell. 1979. Composition of mucus released by coral-reef coelenterates. Limnol. Oceanogr. 24:706-714.
13. Glockner, F. O., R. Amann, A. Alfreider, J. Pernthaler, R. Psenner, K. Trebesius, and K.-H. Schleifer. 1996. An in situ hybridization protocol for detection and identification of planktonic bacteria. Syst. Appl. Microbiol. 19:403-406.
14. Hagstrom, A., U. Larsson, P. Horstedt, and S. Normark. 1979. Frequency of dividing cells, a new approach to the determination of bacterial-growth rates in aquatic environments. Appl. Environ. Microbiol. 37:805-812.
15. Hed, J., C. Dahlgren, and I. Rundquist. 1983. A simple fluorescence technique to stain the plasma-membrane of human-neutrophils. Histochemistry 79:105-110. (Pubitemid 14236478)
16. Herndl, G. J., and B. Velimirov. 1986. Microheterotrophic utilization of mucus released by the Mediterranean coral. Cladocora cespitosa. Mar. Biol. 90:363-369.
17. Hobble, J. E., R. J. Daley, and S. Jasper. 1977. Use of Nuclepore filters for counting bacteria by fluorescence microscopy. Appl. Environ. Microbiol. 33:1225-1228.
18. Huettel, M., C. Wild, and S. Gonelli. 2006. Mucus trap in coral reefs: formation and temporal evolution of particle aggregates caused by coral mucus. Mar. Ecol. Prog. Ser. 307:69-84.
19. Johnston, I. S., and F. Rohwer. 2007. Microbial landscapes on the outer tissue surfaces of the reef-building coral Porites compressa. Coral Reefs 26:375-383.
20. Koren, O., and E. Rosenberg. 2006. Bacteria associated with mucus and tissues of the coral Oculina patagonica in summer and winter. Appl. Environ. Microbiol. 72:5254-5259.
21. Koren, O., and E. Rosenberg. 2008. Bacteria associated with the bleached and cave coral. Oculina patagonica. Microb. Ecol. 55:523-529.
22. Lunau, M., A. Lemke, K. Walther, W. Martens-Habbena, and M. Simon. 2005. An improved method for counting bacteria from sediments and turbid environments by epifluorescence microscopy. Environ. Microbiol. 7:961-968.
23. Malfatti, F., T. J. Samo, and F. Azam. 2010. High-resolution imaging of pelagic bacteria by atomic force microscopy and implications for carbon cycling. ISME J. 4:427-439.
24. Meikle, P., G. N. Richards, and D. Yellowlees. 1987. Structural determination of the oligosaccharide side-chains from a glycoprotein isolated from the mucus of the coral. Acropora formosa. J. Biol. Chem. 262:16941-16947.
25. Meikle, P., G. N. Richards, and D. Yellowlees. 1988. Structural investigations on the mucus from 6 species of coral. Mar. Biol. 99:187-193.
26. Minnikin, S. M., and A. Allen. 1973. Cell-surface mucosubstances from trypsin disaggregation of normal and virus-transformed lines of baby hamster kidney-cells. Biochem. J. 134:1123-1126.
27. Pascal, H., and E. Vacelet. 1982. Bacterial utilization of mucus on the coral, reef of Aqaba (Red Sea), vol. 1, p. 669-677. In E. D. Gomez, C. E. Birkeland, R. W. Buddemeier, R. E. Johannes, J. A. Marsh, Jr., and R. T. Tsuda (ed.), Proceedings of the 4th .International Coral Reef Symposium. Marine Science Center, University of the Philippines, Manila, Philippines.
28. Paul, J. H., M. F. Deflaun, and W. H. Jeffrey. 1986. Elevated levels of microbial activity in the coral surface microlayer. Mar. Ecol. Prog. Ser. 33:29-40.
29. Pumper, R. W., P. Fagan, and W. G. Taylor. 1971. Trypsin sensitivity of mammalian cells grown in a serum-free medium. In Vitro 6:266-268.
30. Ritchie, K. B. 2006. Regulation of microbial populations by coral surface mucus and mucus-associated bacteria. Mar. Ecol. Prog. Ser. 322:1-14.
31. Ritchie, K. B., and G. W. Smith. 1995. Preferential carbon utilization by surface bacterial communities from water mass, normal, and white-band diseased Acropora cervicomis. Mol. Mar. Biol. Biotechnol. 4:345-352.
32. Rypien, K. L., J. R. Ward, and F. Azam. 2010. Antagonistic interactions among coral-associated bacteria. Environ. Microbiol. 12:28-39.
33. Torreton, J. P., and P. Dufour. 1996. Bacterioplankton production determined by DNA synthesis, protein synthesis, and frequency of dividing cells Tuamotu atoll lagoons and surrounding ocean. Microb. Ecol. 32:185-202.
34. Vacelet, E., and B. A. Thomassin. 1991. Microbial utilization of coral mucus in long-term in situ incubation over a coral reef. Hydrobiologia 211:19-32.