Novel in vivo-degradable cellulose-chitin copolymer from metabolically engineered gluconacetobacter xylinus

Applied and Environmental Microbiology

Volumn 76, Issue 18, 2010, Pages 6257-6265

  Yadav, Vikas ^a,c   Paniliatis, Bruce J ^a   Shi, Hai ^b   Lee, Kyongbum ^b   Cebe, Peggy ^b   Kaplan, David L ^a,b  

^a Tufts University   (United States)

^b TUFTS UNIVERSITY   (United States)

^c HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL CELLULOSE; BIOMASS CONVERSION; CELLULAR METABOLITES; COMMONLY USED; DE NOVO SYNTHESIS; DEGRADABILITY; GLUCONACETOBACTER XYLINUS; HUMAN BODIES; IN-VITRO; IN-VIVO; METABOLIC ENGINEERING; MODIFIED CELLULOSE; N-ACETYLGLUCOSAMINE; POTENTIAL APPLICATIONS; RATIONAL REDESIGN; SUBCUTANEOUS IMPLANTS;

BACTERIOLOGY; BIOCONVERSION; BIOMECHANICS; BIOMOLECULES; BIOPOLYMERS; ENZYMES; MECHANICAL PROPERTIES; METABOLISM;

CELLULOSE;

BIOPOLYMER; CELLULOSE; CHITIN; LYSOZYME; PRIMER DNA;

BACTERIUM; BIODEGRADATION; BIOMASS; BIOTRANSFORMATION; CELLULOSE; CHITIN; ENZYME ACTIVITY; GENETIC ENGINEERING; HOMINID; HYDROLYSIS; METABOLITE; POLYMER; RODENT;

ANALYSIS OF VARIANCE; ANIMAL; ARTICLE; ATOMIC FORCE MICROSCOPY; BIOENGINEERING; BIOSYNTHESIS; ELECTROPORATION; GENETICS; GLUCONACETOBACTER XYLINUS; INFRARED SPECTROSCOPY; LIQUID CHROMATOGRAPHY; METABOLISM; METHODOLOGY; MOLECULAR CLONING; MOUSE; PLASMID; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SCANNING ELECTRON MICROSCOPY; TANDEM MASS SPECTROMETRY; ULTRASTRUCTURE; X RAY DIFFRACTION;

ANALYSIS OF VARIANCE; ANIMALS; BIOENGINEERING; BIOPOLYMERS; CELLULOSE; CHITIN; CHROMATOGRAPHY, LIQUID; CLONING, MOLECULAR; DNA PRIMERS; ELECTROPORATION; GLUCONACETOBACTER XYLINUS; MICE; MICROSCOPY, ATOMIC FORCE; MICROSCOPY, ELECTRON, SCANNING; MURAMIDASE; PLASMIDS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SPECTROSCOPY, FOURIER TRANSFORM INFRARED; TANDEM MASS SPECTROMETRY; X-RAY DIFFRACTION;

BACTERIA (MICROORGANISMS); GLUCONACETOBACTER XYLINUS; MUS;

EID: 77956836019     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.00698-10     Document Type: Article

References (43)

1. Cannon, R. E., and S. M. Anderson. 1991. Biogenesis of bacterial cellulose. Crit. Rev. Microbiol. 17: 435-447.
2. Chien, L.-J., H.-T. Chen, P.-F. Vang, and C.-K. Lee. 2006. Enhancement of cellulose pellicle production by constitutively expressing Vitreoscilla hemoglobin in Acetobacter xylinum. Biotechnol. Prog. 22: 1598-1603.
3. Chipman, D. M., and N. Sharon. 1969. Mechanism of lysozyme action. Science 165: 454-465.
4. Czaja, W., A. Kiystynowicz, S. Bielecki, and R. M. Brown, Jr. 2006. Microbial cellulose: the natural power to heal wounds. Biomaterials 27: 145-151.
5. Czaja, W. K., D. J. Young, M. Kawecki, and R. M. Brown, Jr. 2007. The future prospects of microbial cellulose in biomedical applications. Biomacromolecules 8:1-12.
6. Dien, B. S., M. A. Cotta, and T. W. Jeffries. 2003. Bacteria engineered for fuel ethanol production: current status. Appl. Microbiol. Biotechnol. 63:258.
7. Dieter, K., S. Dieter, U. Ulrike, and M. Silvia. 2001. Bacterial synthesized cellulose: artificial blood vessels for microsurgery. Prog. Polym. Sci. 26:1561-1603.
8. Doktycz, M. J., C. J. Sullivanc, P. R. Hoytc, D. A. Pelletiera, S. Wud, and D. P. Allison. 2003. AFM. imaging of bacteria in liquid media immobilized on gelatin coated mica surfaces. Ultramicroscopy 97: 209-216.
9. Hassler, R. A., and D. H. Doherty. 1990. Genetic engineering of polysaccharide structure: production of variants of xanthan gum in Xanthomonas campestris. Biotechnol. Prog. 6:182-187.
10. Helenius, G., H. Bäckdahl, A. Bodin, U. Nannmark, P. Gatenholm, and B. Risberg. 2006. In vivo biocompatibility of bacterial cellulose. J. Biomed. Mater. Res. 76: 43-438.
11. Hestrin, S., and M. Schramm. 1954. Synthesis of cellulose by Acetobacter xylinum: preparation of freeze dried cells capable of polymerizing glucose to cellulose. Biochem. J. 58: 345-353.
12. Hirai, A., M. Tsuji, and F. Horii. 1997. Culture conditions producing structure entities composed of cellulose I and II in bacterial cellulose. Cellulose 4:239-243.
13. Kataoka, Y., and Y. Kondo. 1998. FT-IR microscopic analysis of changing cellulose crystalline structure during wood cell wall formation. Macromolecules 31:760-764.
14. Kobayashi, S., and M. Ohmae. 2006. Enzymatic polymerization to polysaccharides. Adv. Polym. Sci. 194: 159-210.
15. Kolpak, F. J., and J. Blackwell. 1975. The structure of regenerated cellulose. Macromolecules 8: 563-564.
16. Lee, J. W., F. Deng, W. G. Yeomans, A. L. Allen, R. A. Gross, and D. L. Kaplan. 2001. Direct incorporation of glucosamine and N-acetylglucosamine into exopolymers by Gluconacetobacter xylinus (Acetobacterxylinum) ATCC 10245: production of chitosan-cellulose and chitin-cellulose exopolymers. Appl. Environ. Microbiol. 67: 3970-3975.
17. Martin, P. 1997. Wound healing: aiming for perfect skin regeneration. Science 276: 75-81.
18. Michalodimitrakis, K., and M. Isalan. 2009. Engineering prokaryotic gene circuits. FEMS Microbiol. Rev. 33: 27-37.
19. Nishi, N., A. Ebinna, S.-I. Nishimuraa, A. Tsutsumi, O. Hasegawaa, and S. Tokura. 1986. Highly phosphorylated derivatives of chitin, partially deaeetylated chitin and chitosan as new functional polymers: preparation and characterization. Int. J. Biol. Macromol. 8: 311-317.
20. Ogawa, R., Y. Mlura, S. Tokura, and T. Korlyama. 1992. Susceptibilities of bacterial cellulose containing N-acetylglucosamine residues for cellulolytic and chitinolytic enzymes. Int. J. Biol. Macromol. 14: 343-347.
21. Pang, F.-J., C.-J. He, and Q.-R. Wang. 2003. Preparation and properties of cellulose/chitin blend fiber. J. Appl. Polym. Sci. 90: 3430-3436.
22. Reinecke, F., and A. Steinbüchel. 2009. Ralstonia eutropha strain H16 as model organism for PHA metabolism and for biotechnological production of technically interesting biopolymers. J. Mol. Microbiol. Biotechnol. 16:91-108.
23. Ross, P., R. Mayer, and M. Benziman. 1991. Cellulose biosynthesis and function in bacteria. Microbiol. Rev. 55: 35-58.
24. Rubin, E. M. 2008. Genomics of cellulosic biofuels. Nature 454:841-845.
25. Schubert, C. 2006. Can biofuels finally take center stage? Nat. Biotechnol. 24: 777-784.
26. Shaw, A. J., K. K. Podkaminer, S. G. Desal, J. S. Bardsley, S. R. Rogers, P. G. Thorne, D. A. Hogsett, and L. R. Lynd. 2008. Metabolic engineering of a thermophilic bacterium to produce ethanol at high yield. Proc. Natl. Acad. Sci. U. S. A. 105: 13769-13774.
27. Shirai, A., M. Takahashi, H. Kaneko, S.-l. Nishimura, M. Ogawa, N. Nishi, and S. Tokura. 1994. Biosynthesis of a novel polysaccharide by Acetobacter xylinum. Int. J. Biol. Macromol. 16: 297-300.
28. Span, P. N., M.-J. J. M. Pouwels, A. J. Olthaar, R. R. Bosch, A. R. M. M. Hermus, and C. G. J. Sweep. 2001. Assay for hexosamine pathway intermediates (UDP-GlcNAc) in small samples of human muscle tissue. Clin. Chem. 47: 944-946.
29. Steinbuchel, A., and T. Lutke-Eversloh. 2003. Metabolic engineering and pathway construction for biotechnological production of relevant polyhydroxyalkanoates (PHA) in microorganisms. Biochem. Eng. J. 14: 454-459.
30. Stickten, M. B. 2008. Plant genetic engineering for biofuel production: towards affordable cellulosic ethanol. Nat. Rev. Genet. 9: 433-443.
31. Sugiyama, J., R. Vuong, and H. Chanzy. 1991. Electron diffraction study on the two crystalline phases occurring in native cellulose from an algal cell wall. Macromolecules 24:4168-4175.
32. Suzuki, Y., and Y. Makino. 1999. Mucosal drug delivery using cellulose derivatives as a functional polymer. J. Control. Release 62: 101-107.
33. Svensson, A., E. Nicklasson, T. Harrah, B. Panilaitis, D. L. Kaplan, M. Brittberg, and P. Gatenholm. 2005. Bacterial cellulose as a potential scaffold for tissue engineering of cartilage. Biomaterials 26: 419-431.
34. Tokoh, C., K. Takabe, M. Fujita, and H. Salki. 1998. Cellulose synthesized by Acetobacter xylinum in the presence of glucomannan. Cellulose 5: 249-261.
35. Tomonori, N., N. Tonouchi, T. Konishi, Y. Kojima, T. Tsuchida, F. Yoshinaga, F. Sakai, and T. Hayashi. 1999. Enhancement of cellulose production by expression of sucrose synthase in Acetobacter xylinum. Proc. Natl. Acad. Sci. U. S. A. 96: 14-18.
36. van Die, I., R. D. Cummings, A. van Tetering, C. H. Hokke, C. A. M. Koeleman, and D. H. van den Eijnden. 2000. Identification of a novel UDPGlc:Glc.NAc β1→4-glucosyltransferase in Lymnaea stagnalis that may be involved in the synthesis of complex-type oligosaccharide chains. Glycobiology 10: 263-271.
37. van Eikeren, P., and H. McLaughlin. 1977. Analysis of the lysozyme-catalyzed hydrolysis and transglyeosylation of N-acetyl-D-glucosamine oligomers by high-pressure liquid chromatography. Anal. Biochem. 77: 513-522.
38. Wada, M., J. Sugiyama, and T. Okano. 1993. Native celluloses on the basis of two crystalline phase (Iα/Iβ) system. J. Appl. Polym. Sci. 49:1491-1496.
39. Watts, K. T., B. N. Mijts, and C. Schmidt-Dannert. 2003. Current andemerging approaches for natural biosynthesis in microbial cells. Adv. Synth. Catal. 347: 927-940.
40. Wong, H. C., A. L. Fear, R. D. Calhoon, G. H. Eichinger, R. Mayer, D. Amikam, M. Benziman, D. H. Gelfand, J. H. Meade, and A. W. Emerick. 1990. Genetic organization of the cellulose synthase operon in Acetobacter xylinum. Proc. Natl. Acad. Sci. U. S. A. 87: 8130-8134.
41. Yamamoto, H., F. Horii, and A. Hirai. 1996. In situ crystallization of bacterial cellulose. II. Influences of polymeric additives with different molecular weights on the formation of celluloses Iα and Iβ at the early stage of incubation. Cellulose 3: 229-242.
42. Yamamoto, H., F. Horii, and H. Odani. 1989. Structural changes of native cellulose crystals induced by annealing in aqueous alkaline and acidic solutions at high temperatures. Macromolecules 22: 4130-4132.
43. Zhang, M., C. Eddy, K. Deanda, M. Flnkelstein, and S. Picataggio. 1995. Metabolic engineering of a pentose metabolism pathway in ethanologenic Zymomonas mobilis. Science 267: 240-243.