Cloning, expression, purification and physical and kinetic characterization of the phosphoenolpyruvate carboxylase from orange (Citrus sinensis osbeck var. Valencia) fruit juice sacs

Plant Science

Volumn 179, Issue 5, 2010, Pages 527-535

  Perotti, Valeria E ^a   Figueroa, Carlos M ^b   Andreo, Carlos S ^a   Iglesias, Alberto A ^a   Podestá, Florencio E ^b  

^a UNIVERSIDAD NACIONAL DE ROSARIO   (Argentina)

^b Universidad Nacional del Litoral   (Argentina)

Author keywords

Carbon metabolism; Citrus sinensis; Orange fruit; Phosphoenolpyruvate carboxylase

Indexed keywords

CITRUS SINENSIS; MAMMALIA;

EID: 77956759892     PISSN: 01689452     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.plantsci.2010.08.003     Document Type: Article

References (43)

1. Andreo C.S., González D.H., Iglesias A.A. Higher plant phosphoenolpyruvate carboxylase. Structure and regulation. FEBS Lett. 1987, 213:1-8.
2. Izui K., Matsumura H., Furumoto T., Kai Y. Phosphoenolpyruvate carboxylase: a new era of structural biology. Annu. Rev. Plant Biol. 2004, 55:69-84.
3. Kai Y., Matsumura H., Izui K. Phosphoenolpyruvate carboxylase: three-dimensional structure and molecular mechanisms. Arch. Biochem. Biophys. 2003, 414:170-179.
4. Plaxton W.C., Podestá F.E. The functional organization and control of plant respiration. Crit. Rev. Plant Sci. 2006, 25:159-198.
5. Chollet R., Vidal J., O'Leary M.H. Phosphoenolpyruvate carboxylase: a ubiquitous, highly regulated enzyme in plants. Annu. Rev. Plant Physiol. Plant Mol. Biol. 1996, 47:273-902.
6. 2+-bound phosphoenolpyruvate carboxylase from Escherichia coli. FEBS Lett. 1999, 458:93-96.
7. 4-form phosphoenolpyruvate carboxylase from maize. Acta Crystallogr. 1999, 55:1937-1938.
8. Rivoal J., Plaxton W.C., Turpin D.H. Purification and characterization of high- and low-molecular-mass isoforms of phosphoenolpyruvate carboxylase from Chlamydomonas reinhardtii. Kinetic, structural and immunological evidence that the green algal enzyme is distinct from the prokaryotic and higher plant enzymes. Biochem. J. 1998, 331:201-209.
9. Rivoal J., Dunford R., Plaxton W.C., Turpin D.H. Purification and properties of four phosphoenolpyruvate carboxylase isoforms from the green alga Selenastrum minutum: evidence that association of the 102-kDa catalytic subunit with unrelated polypeptides may modify the physical and kinetic properties of the enzyme. Arch. Biochem. Biophys. 1996, 332:47-57.
10. Blonde J.D., Plaxton W.C. Structural and kinetic properties of high and low molecular mass phosphoenolpyruvate carboxylase isoforms from the endosperm of developing castor oilseeds. J. Biol. Chem. 2003, 278:11867-11873.
11. Trípodi K.E.J., Turner W.L., Gennidakis S., Plaxton W.C. In vivo regulatory phosphorylation of novel phosphoenolpyruvate carboxylase Isoforms in endosperm of developing castor oil seeds. Plant Physiol. 2005, 139:969-978.
12. Gennidakis S., Rao S.K., Greenham K., Uhrig R.G., O'Leary B., Snedden W.A., Lu C., Plaxton W.C. Bacterial- and plant-type phosphoenolpyruvate carboxylase polypeptides interact in the hetero-oligomeric Class-2 PEPC complex of developing castor oil seeds. Plant J. 2007, 52:839-849.
13. Borsani J., Budde C.O., Porrini L., Lauxmann M.A., Lombardo V.A., Murray R., Andreo C.S., Drincovich M.F., Lara M.V. Carbon metabolism of peach fruit after harvest: changes in enzymes involved in organic acid and sugar level modifications. J. Exp. Bot. 2009, 60:1823-1837.
14. Falcone Ferreyra M.L., Perotti V., Figueroa C.M., Garrán S., Anderson P.C., Vázquez D.E., Iglesias A.A., Podestá F.E. Carbohydrate metabolism and fruit quality are affected in frost-exposed Valencia orange fruit. Physiol. Plant. 2006, 128:224-236.
15. Mattoo A.K., Sobolev A.P., Neelam A., Goyal R.K., Handa A.K., Segre A.L. Nuclear magnetic resonance spectroscopy-based metabolite profiling of transgenic tomato fruit engineered to accumulate spermidine and spermine reveals enhanced anabolic and nitrogen-carbon interactions. Plant Physiol. 2006, 142:1759-1770.
16. 2 levels in Annona cherimola fruit. J. Agric. Food Chem. 2004, 52:4758-4763.
17. Notton B.A., Blanke M.M. Phosphoenolpyruvate carboxylase in avocado fruit: purification and properties. Phytochemistry 1993, 33:1333-1337.
18. Law R.D., Plaxton W.C. Purification and characterization of a novel phosphoenolpyruvate carboxylase from banana fruit. Biochem. J. 1995, 307:807-816.
19. 2. Phytochemistry 2001, 58:1007-1013.
20. Hall T.C, Ma Y., Buchbinder B.U., Pyne J.W., Sun S.M., Bliss F.A. Messenger RNA for G1 protein of French bean seeds: cell-free translation and product characterization. Proc. Natl. Acad. Sci. U.S.A. 1978, 75:3196-3200.
21. Takahashi-Terada A., Kotera M., Ohshima K., Furumoto T., Matsumura H., Kai Y., Izui K. Maize phosphoenolpyruvate carboxylase. Mutations at the putative binding site for glucose 6-phosphate caused desensitization and abolished responsiveness to regulatory phosphorylation. J. Biol. Chem. 2005, 280:11798-11806.
22. Colombo S.L., Andreo C.S., Podestá F.E. Carbon metabolism in germinating Ricinus communis cotyledons. Purification, characterization and developmental profile of NADP-dependent malic enzyme. Physiol. Plant. 1997, 101:821-826.
23. Smith P.K., Krohn R.I., Hermanson G.T., Mallia A.K., Gartner F.H., Provenzano M.D., Fujimoto E.K., Goeke N.M., Olson B.J., Klenk D.C. Measurement of protein using bicinchoninic acid. Anal. Biochem. 1985, 150:76-85.
24. Brooks S.P.J. A simple computer program with statistical tests for analysis of enzyme kinetics. Biotechniques 1992, 13:906-911.
25. Bollag D.M., Edelstein S.J. Protein Methods 1991, Wiley-Liss Inc., New York, p. 230.
26. Plaxton W.C. Molecular and immunological characterization of plastid and cytosolic pyruvate kinase isozymes from castor-oil-plant endosperm and leaf. Eur. J. Biochem. 1989, 181:443-451.
27. Xu W., Ahmed S., Moriyama H., Chollet R. The importance of the strictly conserved, C-terminal glycine residue in phosphoenolpyruvate carboxylase for overall Catalysis. J. Biol. Chem. 2006, 281:17238-17245.
28. 3 plants: mutations for diminished sensitivity to feedback inhibitors and for increased substrate affinity. J. Exp. Bot. 2008, 59:1811-1818.
29. 4 phosphoenolpyruvate carboxylase. Arch. Biochem. Biophys. 2003, 414:180-188.
30. Trípodi K.E.J., Plaxton W.C. In vivo regulatory phosphorylation of novel phosphoenolpyruvate carboxylase Isoforms in endosperm of developing castor oil seeds. Plant Physiol. 2005, 139:969-978.
31. Hata S., Izui K., Kouchi H. Expression of a soybean nodule-enhanced phosphoenolpyruvate carboxylase gene that shows striking similarity to another gene for a house-keeping isoform. Plant J. 1998, 13:267-273.
32. Echeverria E., Valich J. Carbohydrate and enzyme distribution in protoplasts from valencia orange juice sacs. Phytochemistry 1988, 27:73-76.
33. Moing A., Rothan C., Svanella L., Just D., Diakou P., Raymond P., Gaudillère J.-P., Monet R. Role of phosphoenolpyruvate carboxylase in organic acid accumulation during peach fruit development. Physiol. Plant. 2000, 108:1-10.
34. Guillet C., Just D., Benard N., Destrac-Irvine A., Baldet P., Hernould M., Causse M., Raymond P., Rothan C. A fruit-specific phosphoenolpyruvate carboxylase is related to rapid growth of tomato fruit. Planta 2002, 214:717-726.
35. Lakso A.N., Kliewer W.M. Physical properties of phosphoenolpyruvate carboxylase and malic enzyme in grape berries. Am. J. Enol. Viticult. 1975, 26:75-78.
36. Law R.D., Plaxton W.C. Regulatory phosphorylation of banana fruit phosphoenolpyruvate carboxylase by a copurifying phosphoenolpyruvate carboxylase-kinase. Eur. J. Biochem. 1997, 247:642-651.
37. Podestá F.E., Andreo C.S. Maize leaf phosphoenolpyruvate carboxylase: oligomeric state and activity in the presence of glycerol. Plant Physiol. 1989, 90:427-433.
38. Podestá F.E., Plaxton W.C. Regulation of carbon metabolism in germinating Ricinus communis cotyledons. II. Properties of phosphoenolpyruvate carboxylase and cytosolic pyruvate kinase associated with the regulation of glycolysis and nitrogen assimilation. Planta 1994, 194:406-417.
39. Moraes T.F., Plaxton W.C. Purification and characterization of phosphoenolpyruvate carboxylase from Brassica napus (rapeseed) suspension cell cultures: implications for phosphoenolpyruvate carboxylase regulation during phosphate starvation, and the integration of glycolysis with nitrogen assimilation. Eur. J. Biochem. 2000, 267:4465-4476.
40. Katz E., Fon M., Lee Y., Phinney B., Sadka A., Blumwald E. The citrus fruit proteome: insights into citrus fruit metabolism. Planta 2007, 226:989-1005.
41. Roe B., Davis P.L., Bruemmer J.H. Pyruvate metabolism during maturation of hamlin oranges. Phytochemistry 1984, 23:713-717.
42. Tovar-Méndez A., Mujica-Jiménez C., Muñoz-Clares R.A. Physiological implications of the kinetics of maize leaf phosphoenolpyruvate carboxylase. Plant Physiol. 2000, 123:149-160.
43. Willeford K.O., Wu M.-X., Meyer C.R., Wedding R.T. The role of oligomerization in regulation of maize phosphoenolpyruvate carboxylase activity: influence of Mg-PEP and malate on the oligomeric equilibrium of PEP carboxylase. Biochem. Biophys. Res. Commun. 1990, 168:778-785.