Detection of somaclonal variants in somatic embryogenesis-regenerated plants of Vitis vinifera by flow cytometry and microsatellite markers

Plant Cell, Tissue and Organ Culture

Volumn 103, Issue 1, 2010, Pages 49-59

  Prado, María Jesús ^a   Rodriguez, Eleazar ^b   Rey, Laura ^a   González, María Victoria ^c   Santos, Conceição ^b   Rey, Manuel ^a  

^a UNIVERSITY OF VIGO   (Spain)

^b UNIVERSITY OF AVEIRO   (Portugal)

^c UNIVERSITY OF SANTIAGO DE COMPOSTELA   (Spain)

Author keywords

Flow cytometry; Microsatellites; Somaclonal variation; Somatic embryogenesis; Tetraploid; Vitis vinifera

Indexed keywords

MICROSATELLITES; SOMACLONAL VARIATION; SOMATIC EMBRYOGENESIS; TETRAPLOID; VITIS VINIFERA;

CELL CULTURE; FLOW CYTOMETRY; FRUITS; GENES; PLANT CELL CULTURE;

PLANTS (BOTANY);

VITACEAE; VITIS; VITIS VINIFERA;

EID: 77956742124     PISSN: 01676857     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11240-010-9753-1     Document Type: Article

References (45)

1. Borrego J, De Andrés MT, Gómez JL, Ibáñez J (2002) Genetic study of Malvasía and Torrontés groups through molecular markers. Am J Enol Vitic 53: 125-130.
2. Bowers JE, Dangl GS, Vignani R, Meredith CP (1996) Isolation and characterization of new polymorphic simple sequence repeat loci in grape (Vitis vinifera L). Genome 39: 628-633. doi: 10. 1139/g96-080.
3. Brown S, Bergounioux C, Tallet S, Marie D (1991) Flow cytometry of nuclei for ploidy and cell cycle analysis. In: Negrutiu I, Gharti-Chherti G (eds) A laboratory guide for cellular and molecular plant biology. Birkhäuser, Basel, pp 326-345.
4. Couselo JL, Varela P, Rey M (2006) Effect of benzyladenine concentration and double-phase culture system on in vitro multiplication of adult Albariño plants. Am J Enol Vitic 57: 109-112.
5. Doležel J, Sgorbati S, Lucretti S (1992) Comparison of three DNA fluorochromes for flow cytometric estimation of nuclear DNA content in plants. Physiol Plant 85: 625-631. doi: 10. 1111/j. 1399-3054. 1992. tb04764. x.
6. Doležel J, Bartos J, Voglmayr H, Greilhuber J (2003) Nuclear DNA content and genome size of trout and human. Cytometry Part A 51A: 127-128. doi: 10. 1002/cyto. a. 10013.
7. Endemann M, Hristoforoglu K, Stauber T, Wilhelm E (2001) Assessment of age-related polyploidy in Quercus robur L. somatic embryos and regenerated plants using DNA flow cytometry. Biol Plant 44: 339-345. doi: 10. 1023/A: 1012426306493.
8. Etienne H, Bertrand B (2003) Somaclonal variation in Coffea arabica: effects of genotype and embryogenic cell suspension age on frequency and phenotype of variants. Tree Physiol 23: 419-426. doi: 10. 1093/treephys/23. 6. 419.
9. Faure O, Nougarède A (1993) Nuclear DNA content of somatic and zygotic embryos of Vitis vinifera cv. Grenache noir at the torpedo stage-flow cytometry and in situ DNA microspectrophotometry. Protoplasma 176: 145-150. doi: 10. 1007/BF01378951.
10. Faure O, Aarrouf J, Nougarède A (1996) Ontogenesis, differentiation and precocious germination in anther-derived somatic embryos of grapevine (Vitis vinifera L.)-proembryogenesis. Ann Bot 78: 23-28.
11. Fernández MP, Núñez Y, Ponz F, Hernáiz S, Gallego FJ, Ibáñez J (2008) Characterization of sequence polymorphisms from microsatellite flanking regions in Vitis spp. Mol Breed 22: 455-465. doi: 10. 1007/s11032-008-9189-z.
12. Franks T, He FG, Thomas M (1998) Regeneration of transgenic Vitis vinifera L. Sultana plants: genotypic and phenotypic analysis. Mol Breed 4: 321-333. doi: 10. 1023/A: 1009673619456.
13. Franks T, Botta R, Thomas MR, Franks J (2002) Chimerism in grapevines: implications for cultivar identity, ancestry and genetic improvement. Theor Appl Genet 104: 192-199. doi: 10. 1007/s001220100683.
14. Gao LZ, Zhang CH, Jia JZ, Dong YS (2002) Assessment of population genetic structure in common wild rice Oryza rufipogon Griff. using microsatellite and allozyme markers. Theor Appl Genet 106: 173-180. doi: 10. 1007/s00122-002-1027-9.
15. González-Andrés F, Martín JP, Yuste J, Rubio JA, Arranz C, Ortiz JM (2007) Identification and molecular biodiversity of autochthonous grapevine cultivars in the 'Comarca del Bierzo', León, Spain. Vitis 46: 71-76.
16. Ibáñez J, De Andrés MT, Molino A, Borrego J (2003) Genetic study of key Spanish grapevine varieties using microsatellite analysis. Am J Enol Vitic 54: 22-30.
17. Kuksova VB, Piven NM, Gleba YY (1997) Somaclonal variation and in vitro induced mutagenesis in grapevine. Plant Cell Tissue Org Cult 49: 17-27. doi: 10. 1023/A: 1005830305206.
18. Larkin PJ, Scowcroft WR (1981) Somaclonal variation: a novel source of variability from cell cultures for plant improvement. Theor Appl Genet 60: 197-214. doi: 10. 1007/BF02342540.
19. Leal F, Loureiro J, Rodriguez E, Pais MS, Santos C, Pinto-Carnide O (2006) Nuclear DNA content of Vitis vinifera cultivars and ploidy level analyses of somatic embryo-derived plants obtained from anther culture. Plant Cell Rep 25: 978-985. doi: 10. 1007/s00299-006-0162-1.
20. Levinson G, Gutman GA (1987) Slipped-strand mispairing: a major mechanism for DNA sequence evolution. Mol Biol Evol 4: 203-221.
21. Lima MAVA, Paiva A, Candeias MI (2003) Flow cytometry-a simple method for nuclear DNA content evaluation of Vitis vinifera cv. Periquita somatic embryos obtained from anther cultures. Vitis 42: 99-100.
22. Lodhi MA, Reisch BI (1995) Nuclear DNA content of Vitis species, cultivars, and other genera of the Vitaceae. Theor Appl Genet 90: 11-16. doi: 10. 1007/BF00220990.
23. Lopes T, Pinto G, Loureiro J, Costa A, Santos C (2006) Determination of genetic stability in long-term somatic embryogenic cultures and derived plantlets of cork oak using microsatellite markers. Tree Physiol 26: 1145-1152. doi: 10. 1093/treephys/26. 9. 1145.
24. Lopes T, Capelo A, Brito G, Loureiro J, Santos C (2009) Genetic variability analyses of the somatic embryogenesis induction process in Olea spp. using nuclear microsatellites. Trees 23: 29-36. doi: 10. 1007/s00468-008-0251-6.
25. López M, Cid N, González MV, Cuenca B, Prado MJ, Rey M (2009) Microsatellite and AFLP analysis of autochthonous grapevine cultivars from Galicia (Spain). Am J Enol Vitic 60: 215-222.
26. Loureiro J, Rodriguez E, Doležel J, Santos C (2007) Two new nuclear isolation buffers for plant DNA flow cytometry: a test with 37 species. Ann Bot 100: 875-888. doi: 10. 1093/aob/mcm152.
27. Martín JP, Borrego J, Cabello F, Ortiz JM (2003) Characterization of the Spanish diversity grapevine cultivars using sequence-tagged microsatellite site markers. Genome 46: 10-18. doi: 10. 1139/g02-098.
28. Martín JP, Santiago JL, Pinto-Carnide O, Leal F, Martínez MC, Ortiz JM (2006) Determination of relationships among autochthonous grapevine varieties (Vitis vinifera L.) in the Northwest of the Iberian Peninsula by using microsatellite markers. Genet Resour Crop Evol 53: 1255-1261. doi: 10. 1007/s10722-005-5679-6.
29. Martinelli L, Gribaudo I (2009) Strategies for effective somatic embryogenesis in grapevine: an appraisal. In: Roubelakis-Angelakis KA (ed) Grapevine molecular physiology & biotechnology, 2nd edn. Springer, Dordrecht, pp 461-493.
30. Martinelli L, Zambanini J, Grando MS (2004) Genotype assessment of grape regenerants from floral explants. Vitis 43: 119-122.
31. Marum L, Rocheta M, Maroco J, Oliveira M, Miguel C (2009) Analysis of genetic stability at SSR loci during somatic embryogenesis in maritime pine (Pinuspinaster). Plant Cell Rep 28: 673-682. doi: 10. 1007/s00299-008-0668-9.
32. Michaelson M, Price H, Ellison J, Johnston J (1991) Comparison of plant DNA contents determinated by Feulgen microspectrophotometry and laser flow citometry. Am J Bot 78: 183-188.
33. Moreno-Sanz P, Suárez B, Loureiro MD (2008) Identification of synonyms and homonyms in grapevine cultivars (Vitis vinifera L.) from Asturias (Spain). J Hortic Sci Biotechnol 83: 683-688.
34. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473-497. doi: 10. 1111/j. 1399-3054. 1962. tb08052. x.
35. Nitsch JP, Nitsch C (1969) Haploid plants from pollen grains. Science 163: 85-87. doi: 10. 1126/science. 163. 3862. 85.
36. Petersen KK, Hagberg P, Kristiansen K (2003) Colchicine and oryzalin mediated chromosome doubling in different genotypes of Miscanthus sinensis. Plant Cell Tissue Organ Cult 73: 137-146. doi: 10. 1023/A: 1022854303371.
37. Pinto G, Loureiro J, Lopes T, Santos C (2004) Analysis of the genetic stability of Eucalyptus globulus Labill. somatic embryos by flow cytometry. Theor Appl Genet 109: 580-587. doi: 10. 1007/s00122-004-1655-3.
38. Predieri S (2001) Mutation induced and tissue culture in improving fruits. Plant Cell Tissue Organ Cult 64: 185-210. doi: 10. 1023/A: 1010623203554.
39. Rose JB, Kubba J, Tobutt KR (2000) Chromosome doubling in sterile Syringa vulgaris × S. pinnatifolia hybrids by in vitro culture of nodal explants. Plant Cell Tissue Organ Cult 63: 127-132. doi: 10. 1023/A: 1006472101185.
40. Roy AT, Leggett G, Koutoulis A (2001) In vitro tetraploid induction and generation of tetraploids from mixoploids in hop (Humulus lupulus L.). Plant Cell Rep 20: 489-495. doi: 10. 1007/s002990100364.
41. Schellenbaum P, Mohler V, Wenzel G, Walter B (2008) Variation in DNA methylation patterns of grapevine somaclones (Vitis vinifera L.). BMC Plant Biol 8: 78. doi: 10. 1186/1471-2229-8-78.
42. Wilhelm E, Hristoforoglu K, Fluch S, Burg K (2005) Detection of microsatellite instability during somatic embryogenesis of oak (Quercus robur L.). Plant Cell Rep 23: 790-795. doi: 10. 1007/s00299-004-0891-y.
43. Wu JH, Mooney P (2002) Autotetraploid tangor plant regeneration from in vitro Citrus somatic embryogenic callus treated with colchicine. Plant Cell Tissue Organ Cult 70: 99-104. doi: 10. 1023/A: 1016029829649.
44. Yang XM, Cao ZY, An LZ, Wang YM, Fang XW (2006) In vitro tetraploid induction via colchicine treatment from diploid somatic embryos in grapevine (Vitis vinifera L.). Euphytica 152: 217-224. doi: 10. 1007/s10681-006-9203-7.
45. Yang XM, An LZ, Xiong YC, Zhang JP, Li Y, Xu SJ (2008) Somatic embryogenesis from immature zygotic embryos and monitoring the genetic fidelity of regenerated plants in grapevine. Biol Plant 52: 209-214. doi: 10. 1007/s10535-008-0047-y.