In situ μgISAXS: I. Experimental setup for submicron study of protein nucleation and growth

Biophysical Journal

Volumn 99, Issue 4, 2010, Pages 1256-1261

  Pechkova, Eugenia ^a,b   Gebhardt, Ronald ^c   Riekel, Christian ^c   Nicolini, Claudio ^a,b  

^a UNIVERSITY OF GENOA   (Italy)

^b Fondazione ELBA Rome   (Italy)

^c EUROPEAN SYNCHROTRON RADIATION FACILITY   (France)

Author keywords

[No Author keywords available]

Indexed keywords

LYSOZYME; NANOMATERIAL; THAUMATIN PROTEIN, PLANT; VEGETABLE PROTEIN;

ANIMAL; ARTICLE; CHEMICAL STRUCTURE; CHEMISTRY; CHICKEN; CRYSTALLIZATION; METHODOLOGY; PARTICLE SIZE; SMALL ANGLE SCATTERING; X RAY DIFFRACTION;

ANIMALS; CHICKENS; CRYSTALLIZATION; MODELS, MOLECULAR; MURAMIDASE; NANOSTRUCTURES; PARTICLE SIZE; PLANT PROTEINS; SCATTERING, SMALL ANGLE; X-RAY DIFFRACTION;

EID: 77956605719     PISSN: 00063495     EISSN: 15420086     Source Type: Journal    
DOI: 10.1016/j.bpj.2010.03.069     Document Type: Article

References (19)

1. Riekel, C. 2000. New avenues in x-ray microbeam experiments. Rep. Prog. Phys. 63:233-262.
2. Muller-Buschbaum, P., S. V. Roth, ..., C. Riekel. 2003. Multiple-scaled polymer surfaces investigated with micro-focus grazing-incidence small-angle X-ray scattering. Europhys. Lett. 61:639-645.
3. Yoneda, Y. 1963. Anomalous surface reflection of X-rays. Phys. Rev. 161:2010-2013.
4. Roth, S. V., M. Burghammer, ..., H. Walter. 2003. Self-assembled gradient nanoparticle-polymer multilayers investigated by an advanced characterization method: microbeam grazing incidence x-ray scattering. Appl. Phys. Lett. 82:1935-1937.
5. Dante, S., M. DeRosa, ..., V. I. Troitsky. 1996. Supramolecular ordering of bipolar lipids from Archaea in Langmuir-Blodgett films by low-angle X-ray diffraction. Thin Solid Films. 284:459-463.
6. Pechkova, E., S. V. Roth, ..., C. Nicolini. 2005. MicroGISAXS and protein nanotemplate crystallization: methods and instrumentation. J. Synchrotron Radiat. 12:713-716.
7. Rosenberger, F. 1996. Protein crystallization. J. Cryst. Growth. 166: 40-54.
8. Nicolini, C., and E. Pechkova. 2006. Nanostructured biofilms and bio-crystals. J. Nanosci. Nanotechnol. 6:2209-2236.
9. Nicolini, C., and E. Pechkova. 2006. Structure and growth of ultrasmall protein microcrystals by synchrotron radiation: I. microGISAXS and microdiffraction of P450scc. J. Cell. Biochem. 97:544-552.
10. Pechkova, E., and C. Nicolini. 2006. Structure and growth of ultrasmall protein microcrystals by synchrotron radiation: II. microGISAX and microscopy of lysozyme. J. Cell. Biochem. 97:553-560.
11. Pechkova, E., and C. Nicolini. 2010. In situ study of nanotemplate-induced growth of lysozyme microcrystals by submicron GISAXS. J. Synchrotron Radiat. submitted for publication.
12. Gebhardt, R., E. Pechkova, ..., C. Nicolini. 2010. In situ GISAXS: II. Thaumatin crystal growth kinetic. Biophys. J.
13. Pechkova, E., and C. Nicolini. 2004. Protein nanocrystallography: a new approach to structural proteomics. Trends Biotechnol. 22: 599-602.
14. Pechkova, E., and C. Nicolini. 2003. Proteomics and Nanocrystallogra-phy. Kluwer Academic Press, New York.
15. Pechkova, E., M. Sartore, L. Giacomelli, and C. Nicolini. 2007. Atomic force microscopy of protein films and crystals. Rev. Sci. Instrum. 78:093704.
16. Pechkova, E., and C. Nicolini. 2001. Accelerated protein crystal growth by protein thin film template. J. Cryst. Growth. 231:599-602.
17. Pechkova, E., and C. Nicolini. 2002. Protein nucleation and crystallization by homologous protein thin film template. J. Cell. Biochem. 22:117-122.
18. Pechkova, E., and C. Nicolini. 2002. From art to science in protein crystallization by means of thin-film nanotechnology. Nanotechnology. 13:460-464.
19. Pechkova, E., S. Tripathi, ..., C. Nicolini. 2009. Radiation stability of proteinase K crystals grown by LB nanotemplate method. J. Struct. Biol. 168:409-418.