Comparison of viral load and duration of virus shedding in symptomatic and asymptomatic neonatal rotavirus infections

Journal of Medical Virology

Volumn 82, Issue 10, 2010, Pages 1803-1807

  Ramani, Sasirekha ^a   Sankaran, Premi ^a   Arumugam, Rajesh ^a   Sarkar, Rajiv ^a   Banerjee, Indrani ^a   Mohanty, Ipsita ^a   Jana, Atanu Kumar ^a   Kuruvilla, Kurien Anil ^a   Kang, Gagandeep ^a  

^a CHRISTIAN MEDICAL COLLEGE   (India)

Author keywords

Neonates; Rotavirus; Viral load; Virus shedding

Indexed keywords

ABDOMINAL DISTENSION; AMPLICON; ARTICLE; GASTROINTESTINAL SYMPTOM; GENOTYPE; HUMAN; MAJOR CLINICAL STUDY; NEWBORN; NONHUMAN; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; ROTAVIRUS INFECTION; VIRUS EXCRETION; VIRUS LOAD; VIRUS SHEDDING; VIRUS STRAIN;

FOLLOW-UP STUDIES; GASTROENTERITIS; HUMANS; INFANT; INFANT, NEWBORN; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; ROTAVIRUS; ROTAVIRUS INFECTIONS; TIME FACTORS; VIRAL LOAD; VIRUS SHEDDING;

ROTAVIRUS; ROTAVIRUS STR. I321;

EID: 77956604561     PISSN: 01466615     EISSN: 10969071     Source Type: Journal    
DOI: 10.1002/jmv.21872     Document Type: Article

References (20)

1. Banerjee I, Ramani S, Gladstone BP, Iturriza-Gomara M, Gray JJ, Brown DW, Kang G. 2007. Modification of rotavirus multiplex RT-PCR for the detection of G12 strains based on characterization of emerging G12 rotavirus strains from south India. J Med Virol 79:1413-1421.
2. Boom R, Sol CJ, Salimans MM, Jansen CL, Wertheim-van Dillen PM, van der Noordaa J. 1990. Rapid and simple method for purification of nucleic acids. J Clin Microbiol 28:495-503.
3. Dearlove J, Latham P, Dearlove B, Pearl K, Thomson A, Lewis IG. 1983. Clinical range of neonatal rotavirus gastroenteritis. Br Med J (Clin Res Ed) 286:1473-1475.
4. Flores J, Sears J, Green KY, Perez-Schael I, Morantes A, Daoud G, Gorziglia M, Hoshino Y, Chanock RM, Kapikian AZ. 1988. Genetic stability of rotaviruses recovered from asymptomatic neonatal infections. J Virol 62:4778-4781.
5. Gentsch JR, Glass RI, Woods P, Gouvea V, Gorziglia M, Flores J, Das BK, Bhan MK. 1992. Identification of group A rotavirus gene 4 types by polymerase chain reaction. J Clin Microbiol 30:1365-1373.
6. Gouvea V, Glass RI, Woods P, Taniguchi K, Clark HF, Forrester B, Fang ZY. 1990. Polymerase chain reaction amplification and typing of rotavirus nucleic acid from stool specimens. J Clin Microbiol 28:276-282.
7. Haffejee IE. 1991. Neonatal rotavirus infections. Rev Infect Dis 13:957-962.
8. Iturriza Gomara M, Wong C, Blome S, Desselberger U, Gray J. 2002. Molecular characterization of VP6 genes of human rotavirus isolates: Correlation of genogroups with subgroups and evidence of independent segregation. J Virol 76:6596-6601.
9. Iturriza Gomara M, Kang G, Mammen A, Jana AK, Abraham M, Desselberger U, Brown D, Gray J. 2004. Characterization of G10P[11] rotaviruses causing acute gastroenteritis in neonates and infants in Vellore, India. J Clin Microbiol 42:2541-2547.
10. Iturriza-Gomara M, Kang G, Gray J. 2004. Rotavirus genotyping: Keeping up with an evolving population of human rotaviruses. J Clin Virol 31:259-265.
11. Jothikumar N, Kang G, Hill VR. 2009. Broadly reactive TaqMan assay for real-time RT-PCR detection of rotavirus in clinical and environmental samples. J Virol Methods 155:126-131.
12. Kang G, Iturriza-Gomara M, Wheeler JG, Crystal P, Monica B, Ramani S, Primrose B, Moses PD, Gallimore CI, Brown DW, Gray J. 2004. Quantitation of group A rotavirus by real-time reverse-transcription-polymerase chain reaction:Correlation with clinical severity in children in South India. J Med Virol 73:118-122.
13. Pang XL, Lee B, Boroumand N, Leblanc B, Preiksaitis JK, Yu Ip CC. 2004. Increased detection of rotavirus using a real time reverse transcription- polymerase chain reaction (RT-PCR) assay in stool specimens from children with diarrhea. J Med Virol 72:496-501.
14. Phillips G, Lopman B, Tam CC, Iturriza-Gomara M, Brown D, Gray J. 2009. Diagnosing rotavirus A associated IID: Using ELISA to identify a cut-off for real time RT-PCR. J Clin Virol 44:242-245.
15. Ramani S, Arumugam R, Gopalarathinam N, Mohanty I, Mathew S, Gladstone BP, Jana AK, Kuruvilla KA, Kang G. 2008a. Investigation of the environment and of mothers in transmission of rotavirus infections in the neonatal nursery. J Med Virol 80:1099-1105.
16. Ramani S, Sowmyanarayanan TV, Gladstone BP, Bhowmick K, Asirvatham JR, Jana AK, Kuruvilla KA, Kumar M, Gibikote S, Kang G. 2008b. Rotavirus infection in the neonatal nurseries of a tertiary care hospital in India. Pediatr Infect Dis J 27:719-723.
17. Ramani S, Iturriza-Gomara M, Jana AK, Kuruvilla KA, Gray JJ, Brown DW, Kang G. 2009. Whole genome characterization of reassortant G10P[11] strain (N155) from a neonate with symptomatic rotavirus infection: Identification of genes of human and animal rotavirus origin. J Clin Virol 45:237-244.
18. Richardson S, Grimwood K, Gorrell R, Palombo E, Barnes G, Bishop R. 1998. Extended excretion of rotavirus after severe diarrhoea in young children. Lancet 351:1844-1848.
19. Schwarz BA, Bange R, Vahlenkamp TW, Johne R, Muller H. 2002. Detection and quantitation of group A rotaviruses by competitive and real-time reverse transcription-polymerase chain reaction. J Virol Methods 105:277-285.
20. Sharma R, Hudak ML, Premachandra BR, Stevens G, Monteiro CB, Bradshaw JA, Kaunitz AM, Hollister RA. 2002. Clinical manifestations of rotavirus infection in the neonatal intensive care unit. Pediatr Infect Dis J 21:1099-1105.