Diffusion measurements inside biofilms by image-based fluorescence recovery after photobleaching (FRAP) analysis with a commercial confocal laser scanning microscope

Applied and Environmental Microbiology

Volumn 76, Issue 17, 2010, Pages 5860-5869

  Waharte, François ^a   Steenkeste, Karine ^b   Briandet, Romain ^c   Fontaine Aupart, Marie Pierre ^b  

^a INSTITUT CURIE   (France)

^b INSTITUT DES SCIENCES MOLÉCULAIRES D ORSAY   (France)

^c UMR1319 MICALIS   (France)

Author keywords

[No Author keywords available]

Indexed keywords

CONFOCAL LASER SCANNING MICROSCOPE; DATA ANALYSIS; DIFFUSION COEFFICIENTS; DIFFUSION MEASUREMENTS; FAST ACQUISITION; FLUORESCENCE CORRELATION SPECTROSCOPY; FLUORESCENCE IMAGING; FLUORESCENCE INTENSITIES; FLUORESCENCE RECOVERY AFTER PHOTOBLEACHING; IMAGE INFORMATION; IMAGE-BASED; IN-SITU; MOLECULAR DIFFUSION; MOLECULAR DIFFUSION COEFFICIENT; MOLECULAR LEVELS; SPATIAL INTENSITY; SPATIAL RESOLUTION; STENOTROPHOMONAS MALTOPHILIA;

BIOFILMS; BIOFILTERS; DATA REDUCTION; DIFFUSION IN LIQUIDS; DIFFUSION IN SOLIDS; FLUORESCENCE SPECTROSCOPY; GLUCOSE; IMAGE PROCESSING; LASER APPLICATIONS; MATHEMATICAL MODELS; OPTICAL MICROSCOPY; PHOTOBLEACHING; STRUCTURAL DYNAMICS;

FLUORESCENCE;

BIOFILM; DIFFUSION; FLUORESCENCE; SCANNING ELECTRON MICROSCOPY; SPATIAL RESOLUTION;

ARTICLE; BIOFILM; CONFOCAL MICROSCOPY; DIFFUSION; FLUORESCENCE RECOVERY AFTER PHOTOBLEACHING; GROWTH, DEVELOPMENT AND AGING; IMAGE PROCESSING; LACTOCOCCUS LACTIS; METABOLISM; METHODOLOGY; PHYSIOLOGY; STENOTROPHOMONAS MALTOPHILIA; VALIDATION STUDY;

BIOFILMS; DIFFUSION; FLUORESCENCE RECOVERY AFTER PHOTOBLEACHING; IMAGE PROCESSING, COMPUTER-ASSISTED; LACTOCOCCUS LACTIS; MICROSCOPY, CONFOCAL; STENOTROPHOMONAS MALTOPHILIA;

BACTERIA (MICROORGANISMS); STENOTROPHOMONAS MALTOPHILIA;

EID: 77956588922     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.00754-10     Document Type: Article

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