Clump passaging and expansion of human embryonic and induced pluripotent stem cells on mouse embryonic fibroblast feeder cells

Current Protocols in Stem Cell Biology

Volumn , Issue SUPPL. 14, 2010, Pages

  Hartung, Odelya ^a   Huo, Hongguang ^a   Daley, George Q ^a,b,c,d,e,f   Schlaeger, Thorsten M ^a,b  

^a BOSTON CHILDREN S HOSPITAL   (United States)

^b HARVARD STEM CELL INSTITUTE   (United States)

^c HARVARD MEDICAL SCHOOL   (United States)

^d BRIGHAM AND WOMEN S HOSPITAL   (United States)

^e HOWARD HUGHES MEDICAL INSTITUTE   (United States)

^f Manton Center for Orphan Disease Research   (United States)

Author keywords

bFGF; human embryonic stem cells; human induced pluripotent stem cells; mouse embryonic fibroblasts

Indexed keywords

BASIC FIBROBLAST GROWTH FACTOR;

CELL CULTURE; CELL DIFFERENTIATION; CRYOPRESERVATION; EMBRYONIC STEM CELL; FIBROBLAST; FREEZING; HUMAN; NONHUMAN; PLURIPOTENT STEM CELL; PRIORITY JOURNAL; REVIEW; STEM CELL EXPANSION; THAWING;

EID: 77956469623     PISSN: 19417322     EISSN: 19388969     Source Type: Journal    
DOI: 10.1002/9780470151808.sc01c10s14     Document Type: Review

References (12)

1. Amit, M., Carpenter, M.K., Inokuma, M.S., Chiu, C.P., Harris, C.P., Waknitz, M.A., Itskovitz-Eldor, J., and, Thomson, J.A., 2000. Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged periods of culture. Dev. Biol. 227: 271 - 278.
2. Amit, M., Margulets, V., Segev, H., Shariki, K., Laevsky, I., Coleman, R., and, Itskovitz-Eldor, J., 2003. Human feeder layers for human embryonic stem cells. Biol. Reproduct. 68: 2150 - 2156. (Pubitemid 36687007)
3. Draper, J.S., Smith, K., Gokhale, P., Moore, H.D., Maltby, E., Johnson, J., Meisner, L., Zwaka, T.P., Thomson, J.A., and, Andrews, P.W., 2004. Recurrent gain of chromosomes 17q and 12 in cultured human embryonic stem cells. Nat. Biotechnol. 22: 53 - 54.
4. Lerou, P.H., Yabuuchi, A., Huo, H., Miller, J.D., Boyer, L.F., Schlaeger, T.M., and, Daley, G.Q., 2008. Derivation and maintenance of human embryonic stem cells from poor-quality in vitro fertilization embryos. Nat. Protoc. 3: 923 - 933.
5. Levenstein, M.E., Ludwig, T.E., Xu, R.H., Llanas, R.A., VanDenHeuvel-Kramer, K., Manning, D., and, Thomson, J.A., 2006. Basic fibroblast growth factor support of human embryonic stem cell self-renewal. Stem Cells 24: 568 - 574.
6. Liu, Y., Song, Z., Zhao, Y., Qin, H., Cai, J., Zhang, H., Yu, T., Jiang, S., Wang, G., Ding, M., and, Deng, H., 2006. A novel chemical-defined medium with bFGF and N2B27 supplements supports undifferentiated growth in human embryonic stem cells. Biochem. Biophys. Res. Commun. 346: 131 - 139.
7. Ludwig, T.E., and, Thomson, J.A., 2009. Defined, feeder-independent medium for human embryonic stem cell culture. Curr. Protoc. Stem Cell Biol. 2: 1C.2.1 - 1C.2.16.
8. Ludwig, T.E., Bergendahl, V., Levenstein, M.E., Yu, J., Probasco, M.D., and, Thomson, J.A., 2006. Feeder-independent culture of human embryonic stem cells. Nat. Methods 3: 637 - 646.
9. Martin, M.J., Muorti, A., Gage, F., and, Varki, A., 2005. Human embryonic stem cells express an immunogenic nonhuman sialic acid. Nat. Medicine 11: 228 - 232.
10. Thomson, J.A., Itskovitz-Eldor, J., Shapiro, S.S., Waknitz, M.A., Swiergel, J.J., Marshall, V.S., and, Jones, J.M., 1998. Embryonic stem cell lines derived from human blastocysts. Science 282: 1145 - 1147.
11. Vallier, L., Alexander, M., and, Pedersen, R.A., 2005. Activin/Nodal and FGF pathways cooperate to maintain pluripotency of human embryonic stem cells. J. Cell Sci. 118: 4495 - 4509.
12. Wang, L., Li, L., Menendez, P., Cerdan, C., and, Bhatia, M., 2005. Human embryonic stem cells maintained in the absence of mouse embryonic fibroblasts or conditioned media are capable of hematopoietic development. Blood 105: 4598 - 4603.