Determining the yield and quality of purified RNA

Cold Spring Harbor Protocols

Volumn 5, Issue 6, 2010, Pages

  Rio, Donald C ^a   Ares Jr, Manuel ^a   Hannon, Gregory J ^a   Nilsen, Timothy W ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCENT DYE;

ARTICLE; CAPILLARY ELECTROPHORESIS; CONCENTRATION (PARAMETERS); DNA DETERMINATION; FLUOROMETRY; GEL ELECTROPHORESIS; LIGHT ABSORPTION; MICROSPECTROPHOTOMETRY; NORTHERN BLOTTING; POLYMERASE CHAIN REACTION; PROTEIN RNA BINDING; QUALITY CONTROL; QUANTITATIVE ANALYSIS; QUANTUM YIELD; RNA ANALYSIS; RNA PURIFICATION;

EID: 77956297084     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.top82     Document Type: Article

References (12)

1. Applied Biosystems. 2010. RNA yields from tissues and cells. Appplied Biosystems, Austin, TX, www.ambion.com/techlib/append/rna_yields.html.
2. Klinck R, Bramard A, Inke L, Dufresne-Martin G, Gervais-Bird J, Madden R, Paquet ER, Koh C, Venables JP, Prinos P, et al. 2008. Multiple alternative splicing markers for ovarian cancer. Cancer Res 68: 657-663.
3. Lightfoot S. 2002. Quantitation comparison of total RNA using the Agilent 2100 bioanalyzer, ribogreen analysis and UV spectrometry. Agilent Technologies, Palo Alto, CA, http://www.chem.agilent.com/en-US/Search/Library/_layouts/Agilent/PublicationSummary.aspx?whid=30152&liid=780.
4. Qiagen. 2006. RNeasy mini handbook. Qiagen, Germantown, MD, http://www1.qiagen.com/literature/render.aspx?id=352.
5. Rio DC, Ares M Jr, Hannon GJ, Nilsen TW. 2010a. Purification of RNA by SDS solubilization and phenol extraction. Cold Spring Harb Protoc (this issue). doi: 10.1101/pdb.prot5438.
6. Rio DC, Ares M Jr, Hannon GJ, Nilsen TW. 2010b. Ethanol precipitation of RNA and the use of carriers. Cold Spring Harb Protoc (this issue). doi: 10.1101/pdb.prot5440.
7. Rio DC, Ares M Jr, Hannon GJ, Nilsen TW. 2010c. Removal of DNA from RNA. Cold Spring Harb Protoc (this issue). doi: 10.1101/pdb.prot5443.
8. Rio DC, Ares M Jr, Hannon GJ, Nilsen TW. 2010d. Polyacrylamide gel electrophoresis of RNA. Cold Spring Harb Protoc (this issue). doi: 10.1101/pdb.prot5444.
9. Rio DC, Ares M Jr, Hannon GJ, Nilsen TW. 2010e. Nondenaturing agarose gel electrophoresis of RNA. Cold Spring Harb Protoc (this issue). doi: 10.1101/pdb.prot5445.
10. Sambrook J, Russell DW. 2006. Northern hybridization. Cold Spring Harb Protoc doi: 10.1101/pdb.prot3723.
11. Venables JP, Koh CS, Froehlich U, Lapointe E, Couture S, Inkel L, Bramard A, Paquet ER, Watier V, Durand M, et al. 2008. Multiple and specific mRNA processing targets for the major human hnRNP proteins. Mol Cell Biol 28: 6033-6043.
12. Venables JP, Klinck R, Koh C, Gervais-Bird J, Bramard A, Inkel L, Durand M, Couture S, Froehlich U, Lapointe E, et al. 2009. Cancer-associated regulation of alternative splicing. Nat Struct Mol Biol 16: 717-724.