Purification of the mitotic checkpoint complex (MCC) and the anaphase promoting complex/cyclosome (APC/C) from hela cells

Cold Spring Harbor Protocols

Volumn 5, Issue 6, 2010, Pages

  Sudakin, Valery ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

ANAPHASE PROMOTING COMPLEX; CELL EXTRACT; ENZYME INHIBITOR; MITOTIC CHECKPOINT COMPLEX; UNCLASSIFIED DRUG;

ARTICLE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME ISOLATION; ENZYME PURIFICATION; GEL FILTRATION; HELA CELL; IN VITRO STUDY; ION EXCHANGE CHROMATOGRAPHY; ISOLATION PROCEDURE; UBIQUITINATION; WESTERN BLOTTING;

EID: 77956296194     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot5449     Document Type: Article

References (3)

1. Chan GKT, Jablonski SA, Sudakin V, Hittle JC, Yen TJ. 1999. Human BUBR1 is a mitotic checkpoint kinase that monitors CENP-E functions at kinetochores and binds the cyclosome/APC. J Cell Biol 146: 941-954.
2. Sudakin V, Chan GKT, Yen TJ. 2001. Checkpoint inhibition of the APC/C in HeLa cells is mediated by a complex of BUBR1, BUB3, CDC20, and MAD2. J Cell Biol 154: 925-936.
3. Yu H, Peters J-M, King RW, Page AM, Hieter P, Kirschner MW. 1998. Identification of a cullin homology region in a subunit of the anaphase-promoting complex. Science 279: 1219-1222.