In vitro screen to obtain widespread, transgenic expression in the mouse

Cold Spring Harbor Protocols

Volumn 5, Issue 8, 2010, Pages

  Nagy, Andras ^a   Gertsenstein, Marina ^a   Vintersten, Kristina ^a   Behringer, Richard ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

BETA GALACTOSIDASE; CRE RECOMBINASE;

ARTICLE; CELL DIFFERENTIATION; CELL ISOLATION; CELL LINE; ELECTROPORATION; EMBRYONIC STEM CELL; ENZYME ACTIVITY; FROZEN SECTION; GENE EXPRESSION; IN VITRO STUDY; NONHUMAN; STAINING; TISSUE CULTURE TECHNIQUE; TRANSGENIC MOUSE;

EID: 77956289705     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot4408     Document Type: Article

References (9)

1. Araki K, Araki M, Miyazaki J, Vassalli P. 1995. Site-specific recombination of a trans-gene in fertilized eggs by transient expression of Cre recombinase. Proc Natl Acad Sci 92: 160-164.
2. Nagy A, Gertsenstein M, Vintersten K, Behringer R. 2006a. Electroporating DNA into embryonic stem (ES) cells and selection methods. Cold Spring Harb Protoc doi: 10.1101/pdb.prot4409.
3. Nagy A, Gertsenstein M, Vintersten K, Behringer R. 2006b. Isolating individual embryonic stem (ES) cell colonies by picking. Cold Spring Harb Protoc doi: 10.1101/pdb.prot4410.
4. Nagy A, Gertsenstein M, Vintersten K, Behringer R. 2006c. Freezing embryonic stem (ES) cells in 96-well plates. Cold Spring Harb Protoc doi: 10.1101/pdb.prot4411.
5. Nagy A, Gertsenstein M, Vintersten K, Behringer R. 2006d. Rapid preparation of DNA from cells in 96-well tissue culture dishes. Cold Spring Harb Protoc doi: 10.1101/pdb.prot4413.
6. Nagy A, Gertsenstein M, Vintersten K, Behringer R. 2006e. Injecting blastocysts. Cold Spring Harb Protoc doi: 10.1101/pdb.prot4418.
7. Nagy A, Gertsenstein M, Vintersten K, Behringer R. 2006f. Assembling aggregates between embryonic stem (ES) cells and diploid embryos. Cold Spring Harb Protoc doi: 10.1101/pdb.prot4424.
8. Nagy A, Gertsenstein M, Vintersten K, Behringer R. 2007a. Staining whole mouse embryos for β-galactosidase (lacZ) activity. Cold Spring Harb Protoc doi: 10.1101/pdb.prot4725.
9. Nagy A, Gertsenstein M, Vintersten K, Behringer R. 2007b. Staining frozen mouse embryo sections for β-galactosidase (lacZ) activity. Cold Spring Harb Protoc doi: 10.1101/pdb.prot4726.