Imaging lipids in living cells

Cold Spring Harbor Protocols

Volumn 5, Issue 7, 2010, Pages

  Schultz, Carsten ^a   Neef, Anne B ^a   Gadella, Theodorus W ^a   Goedhart, Joachim ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCENT DYE; FLUOROPHORE; PROTEIN KINASE C GAMMA; UNCLASSIFIED DRUG; YELLOW FLUORESCENT PROTEIN;

ARTICLE; BINDING SITE; CALCIUM CELL LEVEL; CELL MEMBRANE; CHEMICAL LABELING; CHEMICAL REACTION; CYTOPLASM; CYTOSOL; FLUORESCENCE MICROSCOPY; HELA CELL; LIPID ANALYSIS; LIPID TRANSPORT; MOLECULAR IMAGING; PROTEIN LIPID INTERACTION;

EID: 77956283841     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.top83     Document Type: Article

References (41)

1. Agard NJ, Prescher JA, Bertozzi CR. 2004. A strain-promoted [3+2] azide-alkyne cycloaddition for covalent modification of biomolecules in living systems. J Am Chem Soc 126: 15046-15047. [Medline]
2. Alvarez-Venegas R, Sadder M, Hlavacka A, Baluska F, Xia Y, Lu G, Firsov A, Sarath G, Moriyama H, Dubrovsky JG, et al. 2006. The Arabidopsis homolog of trithorax, ATX1, binds phosphatidylinositol 5-phosphate, and the two regulate a common set of target genes. Proc Natl Acad Sci 103: 6049-6054. [Abstract/Free Full Text]
3. Balla T, Varnai P. 2002. Visualizing cellular phosphoinositide pools with GFP-fused protein-modules. Science's STKE 2002: pl3. doi: 10.1126/stke.2002.125.pl3.[Abstract/Free Full Text]
4. Behnia R, Munro S. 2005. Organelle identity and the signposts for membrane traffic. Nature 438: 597-604. [Medline]
5. Chen I, Ting AY. 2005. Site-specific labeling of proteins with small molecules in live cells. Curr Opin Biotechnol 16: 35-40. [Medline]
6. 2+ oscillations and waves by oscillating translocation and activation of protein kinase C. Curr Biol 11: 1089-1097. [Medline]
7. Di Paolo G, De Camilli P. 2006. Phosphoinositides in cell regulation and membrane dynamics. Nature 443: 651-657. [Medline]
8. Dowler S, Currie RA, Campbell DG, Deak M, Kular G, Downes CP, Alessi DR. 2000. Identification of pleckstrin-homology-domain containing proteins with novel phosphoinositide-binding specificities. Biochemistry 351: 19-31.
9. phox. Nat Cell Biol 3: 679-682. [Medline]
10. Gaullier JM, Simonsen A, D'Arrigo A, Bremnes B, Stenmark H, Aasland R. 1998. FYVE fingers bind PtdIns(3)P. Nature 394: 432-433. [Medline]
11. Gozani O, Karuman P, Jones DR, Ivanov D, Cha J, Lugovskoy AA, Baird CL, Zhu H, Field SJ, Lessnick SL, et al. 2003. The PHD finger of the chromatin-associated protein ING2 functions as a nuclear phosphoinositide receptor. Cell 114: 99-111. [Medline]
12. Halet G. 2005. Imaging phosphoinositide dynamics using GFP-tagged protein domains. Biol Cell 97: 501-518. [Medline]
13. Itoh T, De Camilli P. 2006. BAR, F-BAR (EFC) and ENTH/ANTH domains in the regulation of membrane-cytosol interfaces and membrane curvature. Biochim Biophys Acta 1761: 897-912. [Medline]
14. Kremers GJ, Goedhart J, van Munster EB, Gadella TW Jr. 2006. Cyan and yellow super fluorescent proteins with improved brightness, protein folding, and FRET Förster radius. Biochemistry 45: 6570-6580. [Medline]
15. Kremers GJ, Goedhart J, van den Heuvel DJ, Gerritsen HC, Gadella TW Jr. 2007. Improved green and blue fluorescent proteins for expression in bacteria and mammalian cells. Biochemistry 46: 3775-3783. [Medline]
16. Lemmon MA. 2008. Membrane recognition by phospholipid-binding domains. Nat Rev Mol Cell Biol 9: 99-111. [Medline]
17. Neef AB, Schultz C. 2009. Selective fluorescence labeling of lipids in living cells. Angew Chem Int Ed 48: 1498-1500. [Medline]
18. Oancea E, Meyer T. 1998. Protein kinase C as a molecular machine for decoding calcium and diacylglycerol signals. Cell 95: 307-318. [Medline]
19. Oancea E, Teruel MN, Quest AF, Meyer T. 1998. Green fluorescent protein (GFP)-tagged cysteine-rich domains from protein kinase C as fluorescent indicators for diacylglycerol signaling in living cells. J Cell Biol 140: 485-498. [Abstract/Free Full Text]
20. Postma M, Roelofs J, Goedhart J, Gadella TW, Visser AJ, Van Haastert PJ. 2003. Uniform cAMP stimulation of Dictyostelium cells induces localized patches of signal transduction and pseudopodia. Mol Biol Cell 14: 5019-5027. [Abstract/Free Full Text]
21. Prescher JA, Bertozzi CR. 2005. Chemistry in living systems. Nat Chem Biol 1: 13-21. [Medline]
22. Reck-Peterson SL, Derr ND, Stuurman N. 2010. Imaging single molecules using total internal reflection fluorescence microscopy. In Live cell imaging (eds. RD Goldman et al.), 2nd ed, pp. 659-674. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
23. Saxon E, Bertozzi CR. 2000. Cell surface engineering by a modified Staudinger reaction. Science 287: 2007-2010. [Abstract/Free Full Text]
24. Schultz C. 2003. Prodrugs of biologically active phosphate esters. Bioorg Med Chem 11: 885-898. [Medline]
25. Schultz C, Schleifenbaum A, Goedhart J, Gadella TW Jr. 2005. Multiparameter imaging for the analysis of intracellular signaling. Chem Bio Chem 6: 1323-1330. [Medline]
26. Schultz C, Neef AB, Gadella TW Jr, Goedhart J. 2010a. Transfection of cells with DNA encoding a visible fluorescent protein-tagged lipid-binding domain. Cold Spring Harb Protoc (this issue). doi: 10.1101/pdb.prot5457. [Abstract/Free Full Text]
27. Schultz C, Neef AB, Gadella TW Jr, Goedhart J. 2010b. Labeling lipids for imaging in fixed cells. Cold Spring Harb Protoc (this issue). doi: 10.1101/pdb.prot5458.[Abstract/Free Full Text]
28. Schultz C, Neef AB, Gadella TW Jr, Goedhart J. 2010c. Labeling lipids for imaging in live cells. Cold Spring Harb Protoc (this issue). doi: 10.1101/pdb.prot5459.[Abstract/Free Full Text]
29. Shaner NC, Steinbach PA, Tsien RY. 2005. A guide to choosing fluorescent proteins. Nat Methods 2: 905-909. [Medline]
30. Sheetz MP, Sable JE, Dobereiner HG. 2006. Continuous membrane cytoskeleton adhesion requires continuous accommodation to lipid and cytoskeleton dynamics. Annu Rev Biophys Biomol Struct 35: 417-434. [Medline]
31. Sivakumar K, Xie F, Cash BM, Long S, Barnhill HN, Wang Q. 2004. A fluorogenic 1,3-dipolar cycloaddition reaction of 3-azidocoumarins and acetylenes. Org Lett 6: 4603-4606. [Medline]
32. Stauffer TP, Ahn S, Meyer T. 1998. Receptor-induced transient reduction in plasma membrane PtdIns(4,5)P2 concentration monitored in living cells. Curr Biol 8: 343-346. [Medline]
33. Teruel MN, Meyer T. 2000. Translocation and reversible localization of signaling proteins: A dynamic future for signal transduction. Cell 103: 181-184. [Medline]
34. Toomre D. 2010. Cellular imaging using total internal reflection fluorescent microscopy. In Live cell imaging (eds. RD Goldman et al.), 2nd ed, pp. 675-696. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
35. Toomre D, Manstein DJ. 2001. Lighting up the cell surface with evanescent wave microscopy. Trends Cell Biol 11: 298-303. [Medline]
36. van der Wal J, Habets R, Varnai P, Balla T, Jalink K. 2001. Monitoring agonist-induced phospholipase C activation in live cells by fluorescence resonance energy transfer. J Biol Chem 276: 15337-15344. [Abstract/Free Full Text]
37. Varnai P, Rother KI, Balla T. 1999. Phosphatidylinositol 3-kinase-dependent membrane association of the Bruton's tyrosine kinase pleckstrin homology domain visualized in single living cells. J Biol Chem 274: 10983-10989. [Abstract/Free Full Text]
38. Venkateswarlu K, Gunn-Moore F, Oatey PB, Tavare JM, Cullen PJ. 1998. Nerve growth factor- and epidermal growth factor-stimulated translocation of the ADP-ribosylation factor-exchange factor GRP1 to the plasma membrane of PC12 cells requires activation of phosphatidylinositol 3-kinase and the GRP1 pleckstrin homology domain. Biochem J 335: 139-146. [Medline]
39. Verbeek DS, Goedhart J, Bruinsma L, Sinke RJ, Reits EA. 2008. PKC λ mutations in spinocerebellar ataxia type 14 affect C1 domain accessibility and kinase activity leading to aberrant MAPK signaling. J Cell Sci 121: 2339-2349. [Abstract/Free Full Text]
40. 2 activity in cells and organisms. Angew Chem Int Ed 45: 508-512. [Medline]
41. Zacharias DA, Violin JD, Newton AC, Tsien RY. 2002. Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells. Science 296: 913-916. [Abstract/Free Full Text]