Calcium imaging of neuronal circuits in vivo using a circuit-tracing Pseudorabies virus

Cold Spring Harbor Protocols

Volumn 5, Issue 4, 2010, Pages

  Granstedt, Andrea E ^a   Kuhn, Bernd ^a   Wang, Samuel S H ^a   Enquist, Lynn W ^a  

^a PRINCETON UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

NEURONAL CALCIUM SENSOR; PROTEIN G CAMP2; TRACER; UNCLASSIFIED DRUG;

ARTICLE; CALCIUM SIGNALING; CELL FUNCTION; CELL LABELING; CLINICAL PROTOCOL; GANGLION; GENE EXPRESSION SYSTEM; IMAGING SYSTEM; IN VIVO STUDY; ION MONITORING; MOLECULAR IMAGING; MULTIPHOTON MICROSCOPY; NERVE CELL MEMBRANE POTENTIAL; NERVE CONDUCTION; NEUROIMAGING; NONHUMAN; PROTEIN EXPRESSION; PSEUDORABIES HERPETOVIRUS; SUBMANDIBULAR GLAND; VIRUS EXPRESSION; VIRUS RECOMBINANT;

SUID HERPESVIRUS 1;

EID: 77956276067     PISSN: None     EISSN: 15596095     Source Type: Journal    
DOI: 10.1101/pdb.prot5410     Document Type: Article

References (13)

1. Banfield BW, Kaufman JD, Randall JA, Pickard GE. 2003. Development of pseudorabies virus strains expressing red fluorescent proteins: New tools for multisynaptic labeling applications. J Virol 77: 10106-10112.
2. Boldogkoi Z, Balint K, Awatramani GB, Balya D, Busskamp V, Viney TJ, Lagali PS, Duebel J, Pásti E, Tombácz D, et al. 2009. Genetically timed, activity-sensor and rainbow transsynaptic viral tools. Nat Methods 6: 127-130.
3. Card JP. 1998. Practical considerations for the use of pseudorabies virus in transneuronal studies of neural circuitry. Neurosci Biobehav Rev 22: 685-694.
4. Granstedt AE, Szpara ML, Kuhn B, Wang SS-H, Enquist LW. 2009. Fluorescence-based monitoring of in vivo neural activity using a circuit-tracing pseudorabies virus. PLoS One 4: e6923. doi: 10.1371/journal.pone.0006923.
5. Hoogland TM, Kuhn B, Göbel W, Huang W, Nakai J, Helmchen F, Flint J, Wang SS-H. 2009. Radially expanding transglial calcium waves in the intact cerebellum. Proc Natl Acad Sci 106: 3496-3501.
6. 2+ probe composed of a single green fluorescent protein. Nat Biotechnol 19: 137-141.
7. 2+ imaging of dendritic spines. Anal Chem 77: 5861-5869.
8. Purves D, Lichtman JW. 1987. Synaptic sites on reinnervated nerve cells visualized at two different times in living mice. J Neurosci 7: 1492-1497.
9. Smith BN, Banfield BW, Smeraski CA, Wilcox CL, Dudek FE, Enquist LW, Pickard GE. 2000. Pseudorabies virus expressing enhanced green fluorescent protein: A tool for in vitro electrophysiological analysis of transsynaptically labeled neurons in identified central nervous system circuits. Proc Natl Acad Sci 97: 9264-9269.
10. Smith GA, Gross SP, Enquist LW. 2001. Herpesviruses use bidirectional fast-axonal transport to spread in sensory neurons. Proc Natl Acad Sci 98: 3466-3470.
11. Song CK, Enquist LW, Bartness TJ. 2005. New developments in tracing neural circuits with herpesviruses. Virus Res 111: 235-249.
12. 2+ indicator GCaMP2. Proc Natl Acad Sci 103: 4753-4758.
13. Thorne RG, Nicholson C. 2006. In vivo diffusion analysis with quantum dots and dextrans predicts the width of brain extracellular space. Proc Natl Acad Sci 103: 5567-5572.