Microsatellite typing and avidity analysis suggest a common source of infection in herds with epidemic Neospora caninum-associated bovine abortion

Veterinary Parasitology

Volumn 173, Issue 1-2, 2010, Pages 24-31

  Basso, W ^a,b,c,d   Schares, S ^a   Minke, L ^a   Barwald A ^a   Maksimov, A ^a   Peters, M ^e   Schulze, C ^e   Muller M ^e   Conraths, F J ^a   Schares, G ^a  

^a FEDERAL RESEARCH INSTITUTE FOR ANIMAL HEALTH   (Germany)

^b FACULTAD DE CIENCIAS VETERINARIAS   (Argentina)

^c Facultad de Ciencias Naturales e Instituto M Lillo   (Argentina)

^d UNIVERSITY OF ZURICH   (Switzerland)

^e Staatliches Veterinäruntersuchungsamt Arnsberg ^*  (Germany)

Author keywords

Cattle; Epidemic abortion; Multilocus microsatellite typing; Neospora caninum; Nested PCR; P38 Avidity ELISA

Indexed keywords

DNA; IMMUNOGLOBULIN G ANTIBODY; P38 SURFACE ANTIGEN; PARASITE ANTIGEN; UNCLASSIFIED DRUG;

ALLELE; ANIMAL EXPERIMENT; ANIMAL TISSUE; ANTIBODY RESPONSE; ARTICLE; BRAIN TISSUE; CAPILLARY ELECTROPHORESIS; CONTROLLED STUDY; COW; DNA EXTRACTION; DOG; ENZYME LINKED IMMUNOSORBENT ASSAY; EPIDEMIC; FETUS; GENE AMPLIFICATION; GENE LOCUS; GENETIC VARIABILITY; GEOGRAPHY; GERMANY; GESTATIONAL AGE; HERD; MARE; MICROSATELLITE MARKER; NEOSPORA CANINUM; NEOSPOROSIS; NONHUMAN; NUCLEOTIDE REPEAT; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; SEROPREVALENCE; VETERINARY ABORTION;

ABORTION, VETERINARY; ANIMALS; ANTIBODY AFFINITY; CATTLE; CATTLE DISEASES; COCCIDIOSIS; DISEASE OUTBREAKS; FEMALE; GERMANY; IMMUNOGLOBULIN G; MICROSATELLITE REPEATS; MULTILOCUS SEQUENCE TYPING; NEOSPORA; PREGNANCY;

ANIMALIA; BOS; BOVINAE; NEOSPORA CANINUM;

EID: 77956266971     PISSN: 03044017     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.vetpar.2010.06.009     Document Type: Article

References (32)

1. Al-Qassab S., Reichel M.P., Ellis J. A second generation multiplex PCR for typing strains of Neospora caninum using six DNA targets. Mol. Cell. Probes 2010, 24:20-26.
2. Al-Qassab S., Reichel M.P., Ivens A., Ellis J.T. Genetic diversity amongst isolates of Neospora caninum, and the development of a multiplex assay for the detection of distinct strains. Mol. Cell. Probes 2009, 23:132-139.
3. Basso W., Herrmann D.C., Conraths F.J., Pantchev N., Vrhovec M.G., Schares G. First isolation of Neospora caninum from the faeces of a dog from Portugal. Vet. Parasitol. 2009, 159:162-166.
4. Basso W., Schares S., Bärwald A., Herrmann D.C., Conraths F.J., Pantchev N., Vrhovec M.G., Schares G. Molecular comparison of Neospora caninum oocyst isolates from naturally infected dogs with cell culture-derived tachyzoites of the same isolates using nested polymerase chain reaction to amplify microsatellite markers. Vet. Parasitol. 2009, 160:43-50.
5. Beck H.P., Blake D., Darde M.L., Felger I., Pedraza-Diaz S., Regidor-Cerrillo J., Gomez-Bautista M., Ortega-Mora L.M., Putignani L., Shiels B., Tait A., Weir W. Molecular approaches to diversity of populations of apicomplexan parasites. Int. J. Parasitol. 2009, 39:175-189.
6. Björkman C., Alvarez-Garcia G., Conraths F.J., Mattsson J.G., Ortega-Mora L.M., Sager H., Schares G. Neospora caninum IgG avidity tests: an interlaboratory comparison. Vet. Parasitol. 2006, 140:273-280.
7. Björkman C., McAllister M.M., Frössling J., Naslund K., Leung F., Uggla A. Application of the Neospora caninum IgG avidity ELISA in assessment of chronic reproductive losses after an outbreak of neosporosis in a herd of beef cattle. J. Vet. Diagn. Invest. 2003, 15:3-7.
8. Björkman C., Naslund K., Stenlund S., Maley S.W., Buxton D., Uggla A. An IgG avidity ELISA to discriminate between recent and chronic Neospora caninum infection. J. Vet. Diagn. Invest. 1999, 11:41-44.
9. De Marez T., Liddell S., Dubey J.P., Jenkins M.C., Gasbarre L. Oral infection of calves with Neospora caninum oocysts from dogs: humoral and cellular immune responses. Int. J. Parasitol. 1999, 29:1647-1657.
10. Dubey J.P., Buxton D., Wouda W. Pathogenesis of bovine neosporosis. J. Comp. Pathol. 2006, 134:267-289.
11. Dubey J.P., Hattel A.L., Lindsay D.S., Topper M.J. Neonatal Neospora caninum infection in dogs: isolation of the causative agent and experimental transmission. J. Am. Vet. Med. Assoc. 1988, 193:1259-1263.
12. Dubey J.P., Schares G., Ortega-Mora L.M. Epidemiology and control of neosporosis and Neospora caninum. Clin. Microbiol. Rev. 2007, 20:323-367.
13. Gondim L.F., McAllister M.M., Anderson-Sprecher R.C., Björkman C., Lock T.F., Firkins L.D., Gao L., Fischer W.R. Transplacental transmission and abortion in cows administered Neospora caninum oocysts. J. Parasitol. 2004, 90:1394-1400.
14. Guy C.S., Williams D.J.L., Kelly D.F., McGarry J.W., Guy F., Björkman C., Smith R.F., Trees A.J. Neospora caninum in persistently infected, pregnant cows: spontaneous transplacental infection is associated with an acute increase in maternal antibody. Vet. Rec. 2001, 149:443-449.
15. McAllister M.M., Björkman C., Anderson-Sprecher R., Rogers D.G. Evidence of point-source exposure to Neospora caninum and protective immunity in a herd of beef cows. J. Am. Vet. Med. Assoc. 2000, 217:881-887.
16. Müller N., Zimmermann V., Hentrich B., Gottstein B. Diagnosis of Neospora caninum and Toxoplasma gondii infection by PCR and DNA hybridization immunoassay. J. Clin. Microbiol. 1996, 34:2850-2852.
17. Paré J., Thurmond M.C., Hietala S.K. Neospora caninum antibodies in cows during pregnancy as a predictor of congenital infection and abortion. J. Parasitol. 1997, 83:82-87.
18. Pedraza-Diaz S., Marugan-Hernandez V., Collantes-Fernandez E., Regidor-Cerrillo J., Rojo-Montejo S., Gomez-Bautista M., Ortega-Mora L.M. Microsatellite markers for the molecular characterization of Neospora caninum: application to clinical samples. Vet. Parasitol. 2009, 166:38-46.
19. Peters M., Wagner F., Schares G. Canine neosporosis: clinical and pathological findings and first isolation of Neospora caninum in Germany. Parasitol. Res. 2000, 86:1-7.
20. Regidor-Cerrillo J., Gomez-Bautista M., Pereira-Bueno J., Aduriz G., Navarro-Lozano V., Risco-Castillo V., Fernandez-Garcia A., Pedraza-Diaz S., Ortega-Mora L.M. Isolation and genetic characterization of Neospora caninum from asymptomatic calves in Spain. Parasitology 2008, 135:1651-1659.
21. Regidor-Cerrillo J., Pedraza-Diaz S., Gomez-Bautista M., Ortega-Mora L.M. Multilocus microsatellite analysis reveals extensive genetic diversity in Neospora caninum. J. Parasitol. 2006, 92:517-524.
22. Rojo-Montejo S., Collantes-Fernandez E., Regidor-Cerrillo J., Alvarez-Garcia G., Marugan-Hernandez V., Pedraza-Diaz S., Blanco-Murcia J., Prenafeta A., Ortega-Mora L.M. Isolation and characterization of a bovine isolate of Neospora caninum with low virulence. Vet. Parasitol. 2009, 159:7-16.
23. Schares G., Bärwald A., Staubach C., Söndgen P., Rauser M., Schröder R., Peters M., Wurm R., Selhorst T., Conraths F.J. p38-avidity-ELISA: examination of herds experiencing epidemic or endemic Neospora caninum-associated bovine abortion. Vet. Parasitol. 2002, 106:293-305.
24. Schares G., Dubremetz J.F., Dubey J.P., Bärwald A., Loyens A., Conraths F.J. Neospora caninum: identification of 19-, 38-, and 40-kDa surface antigens and a 33-kDa dense granule antigen using monoclonal antibodies. Exp. Parasitol. 1999, 92:109-119.
25. Schares G., Heydorn A.O., Cuppers A., Mehlhorn H., Geue L., Peters M., Conraths F.J. In contrast to dogs, red foxes (Vulpes vulpes) did not shed Neospora caninum upon feeding of intermediate host tissues. Parasitol. Res. 2002, 88:44-52.
26. Schares G., Losson B. Diagnostic applications and recommended diagnostic schemes, Dog. Protozoal Abortion in Farm Ruminants. Guidelines for Diagnosis and Control 2007, 98-103. CABI, UK. L.M. Ortega-Mora, B. Gottstein, F.J. Conraths, D. Buxton (Eds.).
27. Schares G., Rauser M., Söndgen P., Rehberg P., Bärwald A., Dubey J.P., Edelhofer R., Conraths F.J. Use of purified tachyzoite surface antigen p38 in an ELISA to diagnose bovine neosporosis. Int. J. Parasitol. 2000, 30:1123-1130.
28. Stenlund S., Kindahl H., Magnusson U., Uggla A., Björkman C. Serum antibody profile and reproductive performance during two consecutive pregnancies of cows naturally infected with Neospora caninum. Vet. Parasitol. 1999, 85:227-234.
29. Trees A.J., McAllister M.M., Guy C.S., McGarry J.W., Smith R.F., Williams D.J. Neospora caninum: oocyst challenge of pregnant cows. Vet. Parasitol. 2002, 109:147-154.
30. Trees A.J., Williams D.J. Endogenous and exogenous transplacental infection in Neospora caninum and Toxoplasma gondii. Trends Parasitol. 2005, 21:558-561.
31. von Blumröder D., Schares G., Norton R., Williams D.J., Esteban-Redondo I., Wright S., Bjorkman C., Frossling J., Risco-Castillo V., Fernandez-Garcia A., Ortega-Mora L.M., Sager H., Hemphill A., van Maanen C., Wouda W., Conraths F.J. Comparison and standardisation of serological methods for the diagnosis of Neospora caninum infection in bovines. Vet. Parasitol. 2004, 120:11-22.
32. Weston J.F., Williamson N.B., Pomroy W.E. Associations between pregnancy outcome and serological response to Neospora caninum among a group of dairy heifers. New Zealand Vet. J. 2005, 53:142-148.