In vitro growth-inhibitory effect of plant-derived extracts and compounds against Paenibacillus larvae and their acute oral toxicity to adult honey bees

Veterinary Microbiology

Volumn 145, Issue 1-2, 2010, Pages 129-133

  Flesar, Jaroslav ^a   Havlik, Jaroslav ^a   Kloucek, Pavel ^a   Rada, Vojtech ^a   Titera, Dalibor ^a,b   Bednar, Michal ^b   Stropnicky, Michal ^a,b   Kokoska, Ladislav ^a  

^a CZECH UNIVERSITY OF LIFE SCIENCES PRAGUE   (Czech Republic)

^b Bee Research Institute at Dol   (Czech Republic)

Author keywords

Antimicrobial activity; Apis mellifera; Honey bee; Humulus lupulus; Myrtus communis; Paenibacillus larvae; Thymoquinone; Toxicity

Indexed keywords

CAPSAICIN; DICHLOROMETHANE; HEXOBARBITAL; METHANOL; NATURAL PRODUCT; NORDIHYDROGUAIARETIC ACID; PLANT EXTRACT; SANGUINARINE; THYMOQUINONE;

ANIMAL EXPERIMENT; ANTIMICROBIAL ACTIVITY; ARTICLE; BACTERIAL GROWTH; BACTERIAL STRAIN; BROTH DILUTION; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; GROWTH INHIBITION; HONEYBEE; HUMULUS LUPULUS; IN VITRO STUDY; LARVA; MINIMUM INHIBITORY CONCENTRATION; MYRTLE; NONHUMAN; PAENIBACILLUS;

ALDEHYDES; ALKALOIDS; ANIMALS; ANTI-BACTERIAL AGENTS; BEES; BENZOPHENANTHRIDINES; BENZOQUINONES; CAPSAICIN; DOSE-RESPONSE RELATIONSHIP, DRUG; FLAVONOIDS; ISOQUINOLINES; MICROBIAL SENSITIVITY TESTS; NORDIHYDROGUAIARETIC ACID; PAENIBACILLUS; PLANT EXTRACTS; TERPENES;

APIS MELLIFERA; APOIDEA; HUMULUS LUPULUS; MYRTUS COMMUNIS; PAENIBACILLUS LARVAE;

EID: 77956227622     PISSN: 03781135     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.vetmic.2010.03.018     Document Type: Article

References (25)

1. Alippi A.M., Ringuelet J.A., Cerimele E.L., Re M.S., Henning C.P. Antimicrobial activity of some essential oils against Paenibacillus larvae, the causal agent of American foulbrood disease. J. Herbs Spices Med. Plants 1996, 4:9-16.
2. Antunez K., Harriet J., Gende L., Maggi M., Eguaras M., Zunino P. Efficacy of natural propolis extract in the control of American Foulbrood. Vet. Microbiol. 2008, 131:324-331.
3. Aronstein K.A., Hayes G.W. Antimicrobial activity of allicin against honey bee pathogens. J. Apicult. Res. 2004, 43:57-59.
4. Dingman D.W., Stahly D.P. Medium promoting sporulation of Bacillus larvae and metabolism of medium components. Appl. Environ. Microb. 1983, 46:860-869.
5. Eguaras M.J., Fuselli S., Gende L., Fritz R., Ruffinengo S.R., Clement G., Gonzalez A., Bailac P.N., Ponzi M.I. An in vitro evaluation of Tagetes minuta essential oil for the control of the honeybee pathogens Paenibacillus larvae and Ascosphaera apis, and the parasitic mite Varroa destructor. J. Essent. Oil Res. 2005, 17:336-340.
6. European Commission, 2009a. Report of the Meeting of the Terrestrial Animal Health Standards Commission Paris, 2-6 March 2009. Available at (accessed 10.3.2010). http://ec.europa.eu/food/international/organisations/oie_en.htm.
7. European Commission, 2009b. Community Register of Feed Additives pursuant to Regulation (EC) No. 1831/2003. Available at (accessed 10.12.2009). http://ec.europa.eu/food/food/animalnutrition/feedadditives/comm_register_feed_additives_1831-03.pdf.
8. Feldlaufer M.F., Knox D.A., Lusby W.R., Shimanuki H. Antimicrobial activity of fatty acids against Bacillus larvae, the causative agent of American foulbrood disease. Apidologie 1993, 24:95-99.
9. Floris I., Carta C. In vivo activity of Cinnamomum zeylanicum Nees essential oil against Bacillus larvae White. Apicoltura 1990, 6:57-61.
10. Fuselli S.R., de la Rosa S.B.G., Gende L.B., Eguaras M.J., Fritz R. Antimicrobial activity of some Argentinean wild plant essential oils against Paenibacillus larvae larvae, causal agent of American foulbrood (AFB). J. Apicult. Res. 2006, 45:2-7.
11. Fuselli S.R., de la Rosa S.B.G., Gende L.B., Eguaras M.J., Fritz R. Inhibition of Paenibacillus larvae employing a mixture of essential oils and thymol. Rev. Argent. Microbiol. 2006, 38:89-92.
12. Gende L.B., Floris I., Fritz R., Eguaras M.J. Antimicrobial activity of cinnamon (Cinnamomum zeylanicum) essential oil and its main components against Paenibacillus larvae from Argentine. Bull. Insectol. 2008, 61:1-4.
13. Gochnauer T.A., Boch R., Margetts V.J. Inhibition of Ascosphaera apis by citral and geraniol. J. Invertebr. Pathol. 1979, 34:57-61.
14. Gough H.J., McIndoe E.C., Lewis G.B. The use of dimethoate as a reference compound in laboratory acute toxicity tests on honey bees (Apis mellifera L.) 1981-1992. J. Apicult. Res. 1994, 33:119-125.
15. International Commission for Plant-Bee Relationships (ICPBR), 1993. Hazard of Pesticides to Bees. Guideline on test methods for evaluating the side-effects of plant protection products on honeybees. Wageningen, The Netherlands, 1993 ver. 24/10/93 Appendix 22: EPPO G 170. European and Mediterranean Plant Protection Organization.
16. Jorgensen J.H., Turnidge J.D., Washington J.A. Antibacterial susceptibility tests: dilution and disk diffusion methods. Manual of Clinical Microbiology 1999, 1526-1543. ASM Press, Washington, DC, USA. 7th ed. P.R. Murray, E.J. Baron, M.A. Pfaller, F.C. Tenover, R.H. Yolken (Eds.).
17. Lewis K., Ausubel F.M. Prospects for plant-derived antibacterials. Nat. Biotechnol. 2006, 24:1504-1507.
18. Miyagi T., Peng Y.S., Chuang R.Y., Mussen E.C., Spivak M.S., Doi R.H. Verification of oxytetracycline-resistant American foulbrood pathogen Paenibacillus larvae in the United States. J. Invertebr. Pathol. 2000, 75:95-96.
19. Mutinelli F. European legislation governing the authorisation of veterinary medical products with particular reference to the use of drugs for the control of honey bee diseases. Apiacta 2003, 38:156-168.
20. Organisation for Economic Co-operation and Development (OECD), 1998. OECD Guidelines for the testing of chemicals. Guideline 213: Honeybees, acute oral toxicity test. Available at (accessed 21.9.1998). http://www.oecdbookshop.org/oecd/display.asp%3Flang=fr%26sf1=DI%26st1=5LMQCR2K7R34.
21. Pohorecka K. Effect of standardized plant herb extracts on general condition of the honeybee (Apis mellifera L.). Bull. Vet. Inst. Pulawy 2004, 48:415-419.
22. Shaheen F., Ahmad M., Khan S.N., Hussain S.S., Anjum S., Tashkhodjaev B., Turgunov K., Sultankhodzhaev M.N., Choudhary M.I., Atta-Ur-Rahman New alpha-glucosidase inhibitors and antibacterial compounds from Myrtus communis L. Eur. J. Org. Chem. 2006, 10:2371-2377.
23. Schmalreck A.F., Teuber M., Reininger W., Hartl A. Structural features determining antibiotic potencies of natural and synthetic hop bitter resins, their precursors and derivatives. Can. J. Microbiol. 1975, 21:205-212.
24. The World Organisation for Animal Health (OIE), 2008. Manual of diagnostic tests and vaccines for terrestrial animals: American Foulbrood of Honey bees. Available at Accessed 17.7.2008. http://www.oie.int/eng/normes/mmanual/A_summry.htm.
25. Zhang F., Chen B., Xiao S., Yao S. Optimization and comparison of different extraction techniques for sanguinarine and chelerythrine in fruits of Macleaya cordata (Willd). R. Br. Sep. Purif. Technol. 2005, 42:283-290.