Detection of botulinum neurotoxin serotype b at sub mouse LD50 levels by a sandwich immunoassay and its application to toxin detection in milk

PLoS ONE

Volumn 5, Issue 6, 2010, Pages

  Scotcher, Miles C ^a   Cheng, Luisa W ^a   Stanker, Larry H ^a  

^a WESTERN REGIONAL RESEARCH CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BOTULINUM TOXIN; HYBRID PROTEIN; MONOCLONAL ANTIBODY; POLYCLONAL ANTIBODY; RECOMBINANT PROTEIN; BOTULINUM TOXIN B; PRIMER DNA;

ANIMAL EXPERIMENT; ANTIGEN BINDING; ARTICLE; BAGG ALBINO MOUSE; BIOASSAY; CHEMICAL ANALYSIS; CLOSTRIDIUM BOTULINUM; CONTROLLED STUDY; ENZYME IMMUNOASSAY; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; FEMALE; FOOD ANALYSIS; HYBRIDOMA; IMMUNIZATION; LD 50; MILK; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN PURIFICATION; SENSITIVITY AND SPECIFICITY; SEROTYPE; ANIMAL; CHEMISTRY; IMMUNOLOGY; METHODOLOGY;

BACTERIA (MICROORGANISMS); CLOSTRIDIUM BOTULINUM; ESCHERICHIA COLI; MUS;

ANIMALS; ANTIBODIES, MONOCLONAL; BASE SEQUENCE; BOTULINUM TOXINS; DNA PRIMERS; ENZYME-LINKED IMMUNOSORBENT ASSAY; LETHAL DOSE 50; MICE; MILK; RECOMBINANT FUSION PROTEINS;

EID: 77956214420     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0011047     Document Type: Article

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