Quantification of fungal colonization in modified wood: Quantitative real-time PCR as a tool for studies on Trametes versicolor

Holzforschung

Volumn 64, Issue 5, 2010, Pages 645-651

  Pilgård, Annica ^a,b   Alfredsen, Gry ^b   Hietala, Ari ^b  

^a SP TECHNICAL RESEARCH INSTITUTE OF SWEDEN   (Sweden)

^b NORWEGIAN INSTITUTE OF BIOECONOMY RESEARCH   (Norway)

Author keywords

acetylation; CCA; Cu HDO; furfurylation; Pinus sylvestris; qPCR; quantitative real time PCR; thermal modification

Indexed keywords

CCA; CU-HDO; FURFURYLATION; PINUS SYLVESTRIS; QPCR; QUANTITATIVE REAL TIME PCR; THERMAL MODIFICATION;

ACETYLATION; DNA; EXPERIMENTS; FUNGI; GENES; PROTECTIVE COATINGS; TOXICITY; WOOD PRESERVATION; WOOD PRODUCTS;

POLYMERASE CHAIN REACTION;

ACETYLATION; EXPERIMENTATION; FUNGI; GENES; NUCLEIC ACIDS; PINUS SYLVESTRIS; PROTECTIVE COATINGS; THERMAL ANALYSIS; TOXICITY; WOOD PRESERVATION; WOOD PRODUCTS;

FUNGI; PINUS SYLVESTRIS; TRAMETES VERSICOLOR;

EID: 77956205146     PISSN: 00183830     EISSN: 1437434X     Source Type: Journal    
DOI: 10.1515/HF.2010.074     Document Type: Article

References (32)

1. Chou, C.K., Preston, R.D., Levi, M.P. (1974) Fungitoxic action of a copper chromium arsenate wood preservative. Phytopathology 64:335-341.
2. Coyne, K.J., Handy, S.M., Demir, E., Whereat, E.B., Hutchins, D.A., Portune, K.J., Doblin, M.A., Cary, S.C. (2005) Improved quantitative real-time PCR assays for enumeration of harmful algal species in field samples using an exogenous DNA reference standard. Limnol. Oceanogr. Methods 3:381-391.
3. Eikenes, M., Hietala, A., Alfredsen, G., Fossdal, C.G., Solheim, H. (2005) Comparison of chitin, ergosterol and real-time PCR based assays for monitoring colonization of Trametes versicolor in birch wood. Holzforschung 59:568-573.
4. EN 84 (1996) Wood preservatives - Accelerated ageing of treated wood prior to biological testing. Leaching procedure. European Committee for Standardization (CEN), Brussels, Belgium. 7 pp.
5. EN 113, 1996, Wood preservatives - Test method for determining the protective effectiveness against wood destroying basidio-mycetes: Determination of toxic values, European Committee for Standardization (CEN), Brussels, Belgium, 31
6. EPA United States Environmental Protection office of prevention, Pesticides and Toxic substances Agency EPA. 2005. Pesticide fact sheet on Bis-(N-cyclohexyldiazeniumdioxy)-copper-hdo-fs.pdf.
7. Habicht, J., Hantzschel, D., Wittenzellner, J. (2003) Influence of different fixation and ageing procedures on the leaching behaviour of copper from selected wood preservatives in laboratory trials. IRG/WP 03-20264. In: Proceedings, International Research Group on Wood Protection. 18-23 May, Brisbane, Queensland, Australia.
8. Hietala, A.M., Eikenes, M., Kvaalen, H., Solheim, H., Fossdal, C.G. (2003) Multiplex real-time PCR for monitoring Heterobasidion annosum colonization in Norway spruce clones that differ in disease resistance. Appl. Environ. Microbiol. 69:4413-4420.
9. Hietala, A.M., Nagy, N.E., Steffenrem, A., Fossdal, C.G., Kvaalen, H., Solheim, H. (2009) Spatial patterns in hyphal growth and wood degradation within Norway spruce stems colonized by the pathogenic white-rot fungus Heterobasidion parviporum. Appl. Environ. Microbiol. 75:4069-4078.
10. Hill, C. (2006) Wood Modification: Chemical, Thermal and Other Processes, John Wiley and Sons, Chichester, England.
11. Horisawa, S., Sakuma, Y., Doi, S. (2009) Qualitative and quantitative PCR methods using species-specific primers for detection and identification of wood rot fungi. J. Wood Sci. 55:133-138.
12. Jasalavich, C., Otsrofsky, A., Jellison, J. (2000). Detection and identification of decay fungi in spruce wood by restriction fragment length polymorphism analysis of amplified genes encoding rRNA. Appl. Environ. Microbiol. 66:4725-4734.
13. Junga, U., Militz, H. (2005). Particularities in agar block tests of some modified woods caused by different protection and decay principles. In: Proceedings of the 2nd European Conference on Wood Modification, October 6-7th, 2005, Gottingen, Germany. Eds. Militz H., Hill, C. pp. 354-362.
14. Kirker, G.T., Diehl, S.V., Prewitt, M.L., Diehl, W.J. (2008) Microbial community analysis using thermal restricted fragment length polymorphism (T-RFLP) analysis: field study results. IRG/WP 08-20377. In: Proceedings, International Research Group on Wood Protection, 25-29 May, Istanbul, Turkey.
15. Maitre, M., Kutnik, M., Le Bayon, I., Harvengt, L. (2008) Molecular methods: a reliable tool for the identification of wood decay fungi in construction timber. IRG/WP 08-20386. In: Proceedings, International Research Group on Wood Protection, 25-29 May, Istanbul, Turkey.
16. Mumford, R.A., Walsh, K., Barker, I., Boonham, N. (2000) Detection of potato mop top virus and tobacco rattle virus using a multiplex real-time fluorescent reverse-transcription polymerase chain reaction assay. Phytopathology 90:448-453.
17. Olsson, S. (2001) Colonial growth of fungi. In: The Mycota VIII. Biology of the Fungal Cells. Eds. Howard, R., Gow, N. Springer, Heidelberg, Germany.
18. Pilgard, A. (2008) An overview of available DNA-based applications and results relevant for wood protection. In: Proceedings, Nordic Baltic Network in Wood Material Science and Engineering, 13-14 November, Riga, Latvia.
19. Prewitt, L.M., Diehl, S.V., McElroy, T.C., Diehl, W.J. (2008) Comparison of general fungal and basidiomycete-specific ITS primers for identification of wood decay fungi. Forest Prod. J. 58: 66-71.
20. Raberg, U., Hogberg, N., Land, C.J. (2005) Detection and species discrimination using rDNA T-RFLP for identification of wood decay fungi. Holzforschung 59:221-702.
21. Rowell, R.M., Tillman, A.-M., Simonsom, R. (1986a) A simplified procedure for the acetylation of hardwood and softwood flakes for flake board production. J. Wood Chem. Technol. 6:427-448.
22. Rowell, R.M., Simonson, R., Tillman, A.-M. (1986b) A simplified procedure for the acetylation of chips for dimensionally stabilized particleboard products. J. Wood Chem. Technol. 68:740744.
23. Schmidt, O. (2000) Molecular methods for the characterization and identification of the dry rot fungus Serpula lacrymans. Holz-forschung 54:221-228.
24. Schmidt, O., Moreth, U. (1998) Characterization of indoor rot fungi by RAPD analysis. Holzforschung 52:229-233.
25. Schmidt, O., Moreth, U. (1999) Identification of the dry rot fungus, Serpula lacrymans, and the wild merulius, S. himantioides, by amplified ribosomal DNA restriction analysis (ARDRA). Holzforschung 53:123-128.
26. Schmidt, O., Moreth, U. (2000) Species-specific PCA primers in the rDNA-ITS region as a diagnostic tool for Serpula lacrymans. Mycol. Res. 14:429-433.
27. Schmidt, O., Moreth, U. (2003a) Databank of rDNA-ITS sequences from building-rot fungi for their identification. Wood Sci. Tech-nol. 36:429-433.
28. Schmidt, O., Moreth, U. (2003b) Molecular identity of species and isolates of internal pore fungi Antrodia spp. and Oligoporus placenta. Holzforschung 57:120-126.
29. Shafizadeh, F., Chin, P.P.S. (1977) Thermal deterioration of wood. In: Wood Technology: Chemical Aspects, ACS Symposium Series, Vol.43. Ed. Goldstein, I.S. pp. 57-81.
30. Stahlhut, D., Farrell, R., Wakeling, R., Hedley, M. (2008) Wood decay fungi from New Zealand leaky buildings - PCR identification (Part 2) and aerial spore trapping. IRG/WP 08-10649. In: Proceedings, International Research Group on Wood Protection, 25-29 May, Istanbul, Turkey.
31. Venas, T.M. (2008) A study of mechanisms related to the fungal decay protection rendered by wood furfurylation. PhD Dissertation. University of Copenhagen Faculty of Life Science, Danish Technological Institute, Building and Construction. pp. 86.
32. Weller, S.A., Elphinstone, J.G., Smith, N.C., Boonham, N., Stead, D.E. (2000) Detection of Ralstonia solanacearum strains with a quantitative multiplex, real-time, fluorogenic PCR (TaqMan) assay. Appl. Environ. Microbiol. 66:2853-2858