Enhanced immunoassay sensitivity using chemiluminescent acridinium esters with increased light output

Analytical Biochemistry

Volumn 406, Issue 2, 2010, Pages 204-213

  Natrajan, Anand ^a   Sharpe, David ^a   Costello, Jim ^a   Jiang, Qingping ^a  

^a Advanced Technology and Pre Development   (United States)

Author keywords

Acridinium ester; Chemiluminescence; Immunoassay; Improved sensitivity; Increased light output

Indexed keywords

BIOCHEMISTRY; CHEMILUMINESCENCE; ETHYLENE; IMMUNOLOGY;

ACRIDINIUM ESTERS; CLINICAL DIAGNOSTICS; EMPIRICAL STUDIES; ENHANCED IMMUNOASSAY; EXCITATION PROCESS; IMMUNOASSAY; IMPROVED SENSITIVITY; LIGHT OUTPUT;

ESTERS;

ACRIDINIUM ESTER; ANTIBODY CONJUGATE; ETHYLENE GLYCOL; LUMINESCENT AGENT; THEOPHYLLINE; THYROTROPIN; UNCLASSIFIED DRUG;

ARTICLE; CHEMOLUMINESCENCE; CONJUGATE; ELECTRON; EMPIRICISM; IMMUNOASSAY; KINETICS; LIGHT; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; PRIORITY JOURNAL; SPECTRAL SENSITIVITY;

EID: 77956203952     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2010.07.025     Document Type: Article

References (22)

1. Kricka L.J. Clinical applications of chemiluminescence. Anal. Chim. Acta 2003, 500:279-286.
2. Septak M. Acridinium ester-labeled DNA oligonucleotide probes. J. Biolumin. Chemilumin. 1989, 4:351-356.
3. Weeks I., McCapra F., Campbell A.K., Woodhead J.S. Acridinium esters as high specific-activity labels in immunoassays. Clin. Chem. 1983, 29(8):1474-1479.
4. McCapra F., Richardson D.G. The mechanism of chemiluminescence: a new chemiluminescent reaction. Tetrahedron Lett. 1964, 43:3167-3172.
5. Rahut M.M., Sheehan D., Clarke R.A., Roberts B.G., Semsel A.M. Chemiluminescence from the reaction of 9-chlorocarbonyl-10-methyl-acridinium chloride from aqueous hydrogen peroxide. J. Org. Chem. 1965, 30:3587-3592.
6. McCapra F. Chemical mechanisms in bioluminescence. Acc. Chem. Res. 1976, 9(6):201-208.
7. Rak J., Skurski P., Blazejowski J. Toward an understanding of the chemiluminescence accompanying the reaction of 9-carboxy-10-methylacridinium phenyl ester with hydrogen peroxide. J. Org. Chem. 1999, 64:3002-3008.
8. 1-fetoprotein. Clin. Chem. 1983, 29(8):1480-1483.
9. Arnold L.J., Hammond P.W., Wiese W.A., Nelson N.C. Assay formats involving acridinium-ester-labelled DNA probes. Clin. Chem. 1989, 35:1588-1594.
10. Nelson N.C., Cheikh A.B., Matsuda E., Becker M.M. Simultaneous detection of multiple nucleic acid targets in a homogeneous format. Biochemistry 1996, 35:8429-8438.
11. Hammond P.W., Wiese W.A., Waldrop A.A., Nelson N.C. Nucleophilic addition to the 9 position of 9-phenylcarboxylate-10-methylacridinium protects against hydrolysis of the ester. J. Biolumin. Chemilumin. 1991, 6:35-43.
12. Law S.-J., Miller T., Piran U., Klukas C., Chang S., Unger J. Novel poly-substituted aryl acridinium esters and their use in immunoassay. J. Biolumin. Chemilumin. 1989, 4:88-98.
13. Yin D., Cui D., Gao F., He R., He Y., Liu Y., Shen D., Wu M. A rapid and sensitive chemiluminescent immunoassay of total thyroxin with DMAE.NHS-labeled. J. Immunoassay Immunochem. 2008, 29:257-265.
14. Mattingly P.G. Chemiluminescent 10-methyl-acridinium-9-(N-sulphonylcarboxamide) salts. Synthesis and kinetics of light emission. J. Biolumin. Chemilumin. 1991, 6:107-114.
15. S.-J. Law, C.S. Leventis, A. Natrajan, Q. Jiang, P.B. Connolly, J.P. Kilroy, C.R. McCudden, S.M. Tirrell, Functionalized hydrophilic acridinium esters, US Patent No. 5,656,426 (1997).
16. A. Natrajan, D. Sharpe, Q. Jiang, Acridinium ester labels having hydrophilic modifiers, US Patent No. 6,664,043 B2 (2003).
17. A. Natrajan, Q. Jiang, D. Sharpe, J. Costello, High quantum yield acridinium compounds and their uses in improving assay sensitivity, US Patent No. 7,309,615 B2 (2007).
18. A. Natrajan, Q. Jiang, D. Sharpe, S.-J. Law, Near infrared chemiluminescent acridinium compounds and their uses thereof, US Patent No. 6,355,803 B1 (2002).
19. Q. Jiang, J. Xi, A. Natrajan, D. Sharpe, M. Baumann, R. Hilfiker, E. Schmidt, P. Senn, F. Thommen, A. Waldner, A. Alder, S.-J. Law, Chemiluminescent energy transfer conjugates and their uses as labels in binding assays, US Patent No. 6,165,800 (2000).
20. Siegmund M., Bendig J. Die Temperatur- und lo{double acute}sungsmittelabhängigkeit der fluoreszenzquantenausbeuten von N-substituierten acridonon. Ber. Bunsenge. Phys. Chem. 1978, 82:1061-1068.
21. Drukier A.K., Ossetrova N., Schors E., Krasik G., Grigoriev I., Koenig C., Sulkowski M., Holcman J., Brown L.R., Tomaszewski J.E., Schnall M.D., Sainsbury R., Lokshin A.E., Godavac-Zimmerman J. High-sensitivity blood-based detection of breast cancer by multi-photon detection diagnostic proteomics. J. Proteome Res. 2006, 5:1906-1915.
22. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976, 72:248-254.