Development of an RNA extraction protocol for detection of waterborne viruses by reverse transcriptase quantitative PCR (RT-qPCR)

Journal of Virological Methods

Volumn 169, Issue 1, 2010, Pages 8-12

  Jothikumar, N ^a   Sobsey, M D ^b   Cromeans, T L ^a  

^a CENTERS FOR DISEASE CONTROL AND PREVENTION   (United States)

^b University of North Carolina at Chapel Hill   (United States)

Author keywords

Concentration; Enterovirus; Hepatitis A virus; Lysis buffer; RNA extraction; Water

Indexed keywords

BUFFER; LAKE WATER; MACROGOL; RIVER WATER; SILICON DIOXIDE; VIRUS RNA;

ARTICLE; CONTROLLED STUDY; COXSACKIE VIRUS B3; DILUTION; HEPATITIS A VIRUS; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA EXTRACTION; SENSITIVITY AND SPECIFICITY; VIRUS DETECTION; VIRUSES BY HABITAT; WATER SAMPLING; WATERBORNE VIRUS;

CHROMATOGRAPHY, LIQUID; ENTEROVIRUS B, HUMAN; FRESH WATER; HEPATITIS A VIRUS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; SENSITIVITY AND SPECIFICITY; SILICA GEL; VIROLOGY;

COXSACKIEVIRUS; ENTEROVIRUS; HEPATITIS A VIRUS;

EID: 77956187314     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2010.06.005     Document Type: Article

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