Modified sequential multiplex PCR for determining capsular serotypes of invasive pneumococci recovered from Seville

Clinical Microbiology and Infection

Volumn 16, Issue 9, 2010, Pages 1504-1507

  Iraurgui, P ^a   Torres, M J ^a   Gandia, A ^a   Vazquez, I ^a   Cabrera, E G ^a   Obando, I ^a   Garnacho, J ^a   Aznar, J ^a  

^a HOSPITAL UNIVERSITARIO VIRGEN DEL ROCÍO   (Spain)

Author keywords

Capsular polysaccharide; Invasive infection; Multiplex PCR; Serotype; Streptococcus pneumoniae

Indexed keywords

ADULT; ARTICLE; BACTERIAL MEMBRANE; BACTERIAL STRAIN; BACTERIUM IDENTIFICATION; BACTERIUM ISOLATE; BLOOD CULTURE; CEREBROSPINAL FLUID CULTURE; CHILD; CONTROLLED STUDY; CULTURE MEDIUM; HUMAN; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; PLEURA FLUID; PRIORITY JOURNAL; SEROTYPE; SPAIN; STREPTOCOCCUS PNEUMONIAE; SYNOVIAL FLUID;

STREPTOCOCCUS PNEUMONIAE;

EID: 77956107510     PISSN: 1198743X     EISSN: 14690691     Source Type: Journal    
DOI: 10.1111/j.1469-0691.2009.03129.x     Document Type: Article

References (21)

1. Park IH, Pritchard DG, Cartee R, Brandao A, Brandileone MC, Nahm MH. Discovery of a new capsular serotype (6C) within serogroup 6 of Streptococcus pneumoniae. J Clin Microbiol 2007, 45:1225-1233.
2. Robinson KA, Baughman W, Rothrock G. Epidemiology of invasive Streptococcus pneumoniae infections in the United States 1995-1998. JAMA 2001, 285:1729-1735.
3. Hausdorff WP, Bryant J, Paradiso PR, Siber GR. Which pneumococcal serogroups cause the most invasive disease: implications for conjugate vaccine formulation and use, part I. Clin Infect Dis 2000, 30:100-121.
4. Ray GT, Whitney CG, Fireman BH, Ciuryla V, Black SB. Cost-effectiveness of pneumococcal conjugate vaccine: evidence from the first 5 years of use in the United States incorporating herd effects. Pediatr Infect Dis J 2006, 25:494-501.
5. Whitney CG, Pilishivili T, Farley MM. Effectiveness of seven-valent pneumococcal conjugate vaccine against invasive pneumococcal disease: a matched case-control study. Lancet 2006, 368:1495-1502.
6. Lopalco PL, Team E. Use of 7-valent penumococcal conjugate vaccine in EU. Euro Surveill 2006, 11:E061207-E061203.
7. Aguiar SI, Serrano Y, Pinto FR, Melo-Cristino J, Ramírez M. Changes in Streptococcus pneumoniae serotypes causing invasive disease with non-universal vaccination coverage of the seven-valent conjugate vaccine. Clin Microbiol Infect 2008, 14:835-843.
8. Kyaw MH, Lynfield R, Schaffner W. Effect of introduction of the pneumococcal conjugate vaccine on drug-resistant Streptococcus pneumoniae. N Engl J Med 2006, 354:1455-1463.
9. Brito DA, Ramirez M, de Lencastre H. Serotyping Streptococcus pneumoniae by multiplex PCR. J Clin Microbiol 2003, 41:2378-2384.
10. Saha SK, Darmstadt GL, Baqui AH. Identification of serotype in culture negative pneumococcal meningitis using sequential multiplex PCR: implication for surveillance and vaccine design. PLoS ONE 2008, 3. doi 10.1371/journal.pone.0003576
11. Obando I, Arroyo LA, Sanchez-Tatay D. Molecular epidemiology of paediatric invasive pneumococcal disease in southern Spain after the introduction of heptavalent pneumococcal conjugate vaccine. Clin Microbiol Infect 2007, 13:347-348.
12. Rubin LG, Rizvi A. PCR-based assays for detection of Streptococcus pneumoniae serotypes 3, 14, 19F and 23F in respiratory specimens. J Med Microbiol 2004, 53:595-602.
13. Azzari C, Moriondo M, Indolfi G. Molecular detection methods and serotyping performed directly on clinical samples improve diagnostic sensitivity and reveal increased incidence of invasive disease by Streptococcus pneumoniae in Italian children. J Med Microbiol 2008, 57:1205-1212.
14. Antonio M, Hakeem I, Sakareh K, Cheung YB, Adegbola RA. Evaluation of sequential multiplex PCR for direct detection of multiple serotypes of Streptococcus pneumoniae from nasopharyngeal secretions. J Med Microbiol 2009, 58:296-302.
15. Pai R, Gertz RE, Beall B. Sequential multiplex PCR approach for determining capsular serotypes of Streptococcus pneumoniae isolates. J Clin Microbiol 2006, 44:124-131.
16. Morais L, Carvalho MG, Roca A. Sequential multiplex PCR for identifying pneumococcal capsular serotypes from south-Saharan African clinical isolates. J Med Microbiol 2007, 56:1181-1184.
17. Dias CA, Martins Teixeira L, Carvalho MG, Beall B. Sequential multiplex PCR for determining capsular serotypes of pneumococci recovered from Brazilian children. J Med Microbiol 2007, 56:1185-1188.
18. Campos LC, Carvalho MG, Beall BW. Prevalence of Streptococcus pneumoniae serotype 6C among invasive and carriage isolates in metropolitan Salvador, Brazil, from 1996 to 2007. Diagn Microbiol Infect Dis 2009, 65:112-115.
19. Fenoll A, Granizo JJ, Aguilar L. Temporal trends of invasive Streptococcus pneumoniae serotypes and antimicrobial resistance patterns in Spain from 1979 to 2007. J Clin Microbiol 2009, 47:1012-1020.
20. Pai R, Limor J, Beall B. Use of pyrosequencing to differentiate Streptococcus pneumoniae serotypes 6A and 6B. J Clin Microbiol 2005, 43:4820-4822.
21. Jin P, Xiao M, Kong F. Simple, accurate, serotype-specific PCR assay to differentiate Streptococcus pneumoniae serotypes 6A, 6B, and 6C. J Clin Microbiol 2009, 47:2470-2474.