Spontaneous redifferentiation of dedifferentiated human articular chondrocytes on hydrogel surfaces

Tissue Engineering - Part A

Volumn 16, Issue 8, 2010, Pages 2529-2540

  Yang, Jing Jing ^a   Chen, Yong Mei ^a,b   Liu, Jian Fang ^a   Kurokawa, Takayuki ^a   Gong, Jian Ping ^a  

^a HOKKAIDO UNIVERSITY   (Japan)

^b XI'AN JIAOTONG UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

BODY FLUIDS; COPOLYMERIZATION; GELS; GENE EXPRESSION; MONOLAYERS; MONOMERS; ORGANIC POLYMERS; POLYSTYRENES;

CELL MORPHOLOGY; CHARGED MONOMERS; CHONDROCYTES; CLINICAL APPLICATION; COPOLYMER GELS; CROSSLINKED COPOLYMERS; EFFICIENT METHOD; FUNDAMENTAL RESEARCH; HUMAN ARTICULAR CHONDROCYTES; HYDROGEL SURFACES; MOLAR FRACTIONS; N ,N-DIMETHYLACRYLAMIDE; NEUTRAL MONOMERS; POLYSTYRENE SUBSTRATA; SODIUM SALT;

HYDROGELS;

BIOMATERIAL;

ARTICLE; ARTICULAR CARTILAGE; CARTILAGE CELL; CELL DIFFERENTIATION; CELL LINE; CHEMISTRY; CRYSTALLIZATION; CULTURE TECHNIQUE; CYTOLOGY; HUMAN; HYDROGEL; MATERIALS TESTING; METHODOLOGY; PHYSIOLOGY; SURFACE PROPERTY; TISSUE ENGINEERING;

BIOCOMPATIBLE MATERIALS; CARTILAGE, ARTICULAR; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELL LINE; CHONDROCYTES; CRYSTALLIZATION; HUMANS; HYDROGELS; MATERIALS TESTING; SURFACE PROPERTIES; TISSUE ENGINEERING;

EID: 77956067960     PISSN: 19373341     EISSN: 1937335X     Source Type: Journal    
DOI: 10.1089/ten.tea.2009.0647     Document Type: Article

References (47)

1. Lin, Z., Fitzgerald, J.B., Xu, J., Willers, C., Wood, D., Grodzinsky, A.J., and Zheng, M.H. Gene expression profiles of human chondrocytes during passaged monolayer cultivation. J Orthop Res 26, 1230, 2008.
2. Benya, P.D., and Shaffer, J.D. Dedifferentiated chondrocytes reexpress the differentiated collagen phenotype when cultured in agarose gels. Cell 30, 215, 1982.
3. Benya, P.D., Padilla, S.R., and Nimni, M.E. Independent regulation of collagen types by chondrocytes during the loss of differentiated function in culture. Cell 15, 1313, 1978.
4. Huch, K., Stove, J., Puhl, W., and Gunther, K.P. Review and comparison of culture-techniques for articular chondrocytes. Z Orthop Ihre Grenzgeb 140, 145, 2002.
5. Stokes, D.G., Liu, G., Dharmavaram, R., Hawkins, D., Piera-Velazquez, S., and Jimenez, S.A. Regulation of type-II collagen gene expression during human chondrocyte de-differentiation and recovery of chondrocyte-specific pheno-type in culture involves Sry-type high-mobility-group box (SOX) transcription factors. Biochem J 360, 461, 2001.
6. Lin, Z., Willers, C., Xu, J., and Zheng, M.H. The chondrocyte: biology and clinical application. Tissue Eng 12, 1971, 2006.
7. Ballock, R.T., and Reddi, A.H. Thyroxine is the serum factor that regulates morphogenesis of columnar cartilage from isolated chondrocytes in chemically defined medium. J Cell Biol 126, 1311, 1994.
8. Kuettner, K.E., Pauli, B.U., Gall, G., Memoli, V.A., and Schenk, R.K. Synthesis of cartilage matrix by mammalian chondrocytes in vitro. 1. Isolation, culture characteristics, and morphology. J Cell Biol 93, 743, 1982.
9. Lefebvre, V., Huang, W., Harley, V.R., Goodfellow, P.N., and de Crombrugghe, B. SOX9 is a potent activator of the chondrocyte-specific enhancer of the pro alpha1 (II) collagen gene. Mol Cell Biol 17, 2336, 1997.
10. Park, Y., Lutolf, M.P., Hubbell, J.A., Hunziker, E.B., and Wong, M. Bovine primary chondrocyte culture in syntheticmatrix metalloproteinase- sensitive poly(ethylene glycol)-based hydrogels as a scaffold for cartilage repair. Tissue Eng 10, 515, 2004.
11. Hu, J.C., and Athanasiou, K.A. Low-density cultures of bovine chondrocytes: effects of scaffold material and culture system. Biomaterials 26, 2001, 2005.
12. Freyria, A.M., Cortial, D., Ronziere, M.C., Guerret, S., and Herbage, D. Influence of medium composition, static and stirred conditions on the proliferation of and matrix protein expression of bovine articular chondrocytes cultured in a 3-D collagen scaffold. Biomaterials 25, 687, 2004.
13. Breinan, H.A., Minas, T., Barone, L., Tubo, R., Hsu, H.P., Shortkroff, S., Nehrer, S., Sledge, C.B., and Spector, M. Histological evaluation of the course of healing of canine articular cartilage defects treated with cultured autologous chondrocytes. Tissue Eng 4, 101, 1998.
14. Diao, H.J., Wang, J.L., Shen, C., Xia, S.H., Guo, T., Dong, L., and Zhang, C. Improved cartilage regeneration utilizing mesenchymal stem cells in TGF-b1 gene-activated scaffolds. Tissue Eng Part A 15, 2687, 2009.
15. Stevens, M.M., Qanadilo, H.F., Langer, R., and Shastri, V.P. A rapid-curing alginate gel system: utility in periosteum-derived cartilage tissue engineering. Biomaterials 25, 887, 2004.
16. Elisseeff, J., Anseth, K., Sims, D., McIntosh, M., Randolph, M., Yaremchuk, M., and Langer, R. Transdermal photo-polymerization of poly(ethylene oxide)-based injectable hy-drogels for tissue-engineered cartilage. Plast Reconstr Surg 104, 1014, 1999.
17. Mercier, N.R., Costantino, H.R., Tracy, M.A., and Bonassar, L.J. A novel injectable approach for cartilage formation in vivo using PLG microsphere. Ann Biomed Eng 32, 418, 2004.
18. Silverman, R.P., Passaretti, D., Huang, W., Randolph, M.A., and Yaremchuk, M.J. Injectable tissue-engineered cartilage using a fibrin glue polymer. Plast Reconstr Surg 103, 1809, 1999.
19. Kellner, K., Lang, K., Papadimitriou, A., Leser, U., Milz, S., Schulz, M.B., Blunk, T., and Göpferich, A. Effects of hedgehog proteins on tissue engineering of cartilage in vitro. Tissue Eng 8, 561, 2002.
20. Hauselmann, H.J., Fernandes, R.J., Mok, S.S., Schmid, T.M., Block, J.A., Aydelotte, M.B., Kuettner, K.E., and Thonar, E.J. Phenotypic stability of bovine articular chondrocytes after long-term culture in alginate beads. J Cell Sci 107, 17, 1994.
21. Martin, I., Vunjak-Novakonic, G., Yang, J., Langer, R., and Freed, L.E. Mammalian chondrocytes expanded in the presence of fibroblast growth factor 2 maintain the ability to differentiate and regenerate three-dimensional cartilaginous tissue. Exp Cell Res 253, 681, 1999.
22. Grigolo, B., Lisignoli, G., Piacentini, A., Fiorini, M., Gobbi, P., Mazzotti, G., Duca, M., Pavesio, A., and Facchini, A. Evidence for redifferentiation of human chondrocytes grown on a hyaluronan-based biomaterial (HYAff 11): molecular, immunohistochemical and ultrastructural analysis. Biomaterials 23, 1187, 2002.
23. Malda, J., van Blitterswijk, C.A., Grojec, M., Martens, D.E., Tramper, J., and Riesle, J. Expansion of bovine chondrocytes on microcarriers enhances redifferentiation. Tissue Eng 9, 939, 2003.
24. Smith, R.L., Carter, D.R., and Schurman, D.J. Pressure and shear differentially alter human articular chondrocyte metabolism: a review. Clin Orthop Relat Res 427 Suppl, S89, 2004.
25. Kawanishi, M., Oura, A., Furukawa, K., Fukubayashi, T., Nakamura, K., Tateishi, T., and Ushida, T. Redifferentiation of dedifferentiated bovine articular chondrocytes enhanced by cyclic hydrostatic pressure under a gas-controlled system. Tissue Eng 13, 957, 2007.
26. Ryan, P.L., Foty, R.A., Kohn, J., and Steinberg, M.S. Tissue spreading on implantable substrates is a competitive outcome of cell-cell vs. cell-substratum adhesivity. Proc Natl Acad Sci USA 98, 4323, 2001.
27. Massia, S.P., and Hubbell, J.A. Human endothelial cell interactions with surface-coupled adhesion peptides on a nonadhesive glass substrate and polymeric biomaterials. J Biomed Mater Res 25, 223, 2004.
28. Re, F., Zanetti, A., Sironi, M., Polentarutti, N., Lanfrancone, L., Dejana, E., and Colotta, F. Inhibition of anchorage-dependent cell spreading triggers apoptosis in cultured human endothelial cell. J Cell Biol 127, 537, 1994.
29. Liaw, L., Almeida, M., Hart, C.E., Schwartz, S.M., and Giachelli, C.M. Osteopontin promotes vascular cell adhesion and spreading and is chemotactic for smooth muscle cells in vitro. Circ Res 74, 214, 1994.
30. Lin, S.J., Jee, S.H., Hsaio, W.C., Lee, S.J., and Young, T.H. Formation of melanocyte spheroids on the chitosan-coated surface. Biomaterials 26, 1413, 2005.
31. Lin, S.J., Hsiao, W.C., Jee, S.H., Yu, H.S., Tsai, T. F., Lai, J.Y., and Young, T.H. Study on the effects of nylon-chitosan-blended membranes on the spheroid-forming activity of human melanocytes. Biomaterials 27, 5079, 2006.
32. Hu, J.C., and Athansiou, K.A. A self-assembling process in articular cartilage tissue engineering. Tissue Eng 12, 969, 2006.
33. Yasuda, K., Kitamura, N., Gong, J.P., Arakaki, K., Kown, H.J., Onodera, S., Chen, Y.M., Kurokawa, T., Kanaya, F., Ohmiya, Y., and Osada, Y. A novel double-network hydrogel induces spontaneous articular cartilage regeneration in vivo in a large osteochondral defect. Macromol Biosci 9, 309, 2009.
34. Chen, Y.M., Yang, J.J., and Gong, J.P. Adhesion, spreading, and proliferation of endothelial cells on charged hydrogels. J Adhes 85, 839, 2009.
35. Chen, Y.M., Shiraishi, N., Satokawa, H., Kakugo, A., Narita, T., Gong, J.P., Osada, Y., Yamamoto, K., and Ando, J. Cultivation of endothelial cells on adhesive protein-free synthetic polymer gels. Biomaterials 26, 4588, 2005.
36. Chen, Y.M., Gong, J.P., Tanaka, M., Yasuda, K., Yamamoto, S., Shimomura, M., and Osada, Y. Tuning of cell proliferation on tough gels by critical charge effect. J Biomed Mater Res A 88, 74, 2009.
37. Chen, Y.M., Ogawa, R., Kakugo, A., Osada, Y., and Gong, J.P. Dynamic cell behavior on synthetic hydrogels with different charge densities. Soft Mater 5, 1804, 2009.
38. Schutyser, E., Struyf, S., Wuyts, A., Put, W., Geboes, K., Grillet, B., Opdenakker, G., and Damme, J.V. Selective induction of CCL18/PARC by staphylococcal enterotoxins in mononuclear cells and enhanced levels in septic and rheumatoid arthritis. Eur J Immunol 31, 3755, 2001.
39. Matthews, J.A., Boland, E.D., Wnek, G.E., Simpson, D.G., and Bowlin, G.L. Electrospinning of collagen type II: a feasibility study. J Bioact Compat Polym 18, 125, 2003.
40. Schnalbel, M., Marlovits, S., Eckhoff, G., Fichtel, I., Gotzen, L., Vecsei, V., and Schlegel, J. Dedifferentiation-associated changes in morphology and gene expression in primary human articular chondrocytes in cell culture. Osteoarthritis Cartilage 10, 62, 2002.
41. Horwitz, A.L., and Dorfman, A. The growth of cartilage cells in soft agar and liquid suspension. J Cell Biol 45, 434, 1970.
42. Xu, Y., Malladi, P., Chiou, M., Bekerman, E., Giaccia, A.J., and Longaker, M.T. In vitro expansion of adipose-derived adult stromal cells in hypoxia enhances early chondrogen-esis. Tissue Eng 13, 2981, 2007.
43. Chen, L., Fink, T., Ebbesen, P., and Zachar, V. Optimized chondrogenesis of ATDC5 cells through sequential regulation of oxygen conditions. Tissue Eng 12, 559, 2006.
44. Steele, J.G., Johnson, G., Mclean, K.M., Beumer, G.J., and Griesser, H.J. Effect of porosity and surface hydrophilicity on migration of epithelial tissue over synthetic polymer. J Biomed Mater Res A 50, 475, 2000.
45. Horbett, T.A., Waldburger, J.J., Ratner, B.D., and Hoffman, A.S. Cell adhesion to a series of hydrophilic-hydrophobic copolymers studied with a spinning disc apparatus. J Biomed Mater Res 22, 383, 1988.
46. Kown, H.J., Yasuda, K., Ohmiya, Y., Honma, K., Chen, Y.M., and Gong, J.P. In vitro differentiation of chondrogenic ATDC5 cells is enhanced by culturing on synthetic hydrogels with various charge densities. Acta Biomater 6, 494, 2010.
47. De Gennes, P.G., Brochard-Wyart, F., and Quéré, D. Capillarity and Wetting Phenomena: Drops, Bubbles, Pearls, Waves. Berlin, Germany: Springer, 2004.