High-content screening of small compounds on human embryonic stem cells

Biochemical Society Transactions

Volumn 38, Issue 4, 2010, Pages 1046-1050

  Barbaric, Ivana ^a   Gokhale, Paul J ^a   Andrews, Peter W ^a  

^a UNIVERSITY OF SHEFFIELD   (United Kingdom)

Author keywords

Drug discovery; Embryonic stem cell; High content screening; High throughput screening; Induced pluripotent stem cell

Indexed keywords

AUTOANALYSIS; CELL DIFFERENTIATION; CELL FATE; CELL SURVIVAL; CONTENT ANALYSIS; CYTOLOGY; EMBRYONIC STEM CELL; GENETIC ANALYSIS; HUMAN; IMAGE ANALYSIS; NONHUMAN; PHENOTYPE; PLURIPOTENT STEM CELL; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REVIEW; SCREENING;

ALGORITHMS; CELL DIFFERENTIATION; CELL SURVIVAL; DRUG DISCOVERY; EMBRYONIC STEM CELLS; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; MODELS, BIOLOGICAL; SMALL MOLECULE LIBRARIES;

IPS;

EID: 77955958949     PISSN: 03005127     EISSN: 14708752     Source Type: Journal    
DOI: 10.1042/BST0381046     Document Type: Review

References (40)

1. Thomson, J.A., Itskovitz-Eldor, J., Shapiro, S.S., Waknitz, M.A., Swiergiel, J.J., Marshall, V.S. and Jones, J.M. (1998) Embryonic stem cell lines derived from human blastocysts. Science 282, 1145-1147
2. Takahashi, K. and Yamanaka, S. (2006) Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126, 663-676 (Pubitemid 44233629)
3. Takahashi, K., Tanabe, K., Ohnuki, M., Narita, M., Ichisaka, T., Tomoda, K. and Yamanaka, S. (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131, 861-872 (Pubitemid 350138099)
4. Murry, C.E. and Keller, G. (2008) Differentiation of embryonic stem cells to clinically relevant populations: lessons from embryonic development. Cell 132, 661-680
5. Niwa, H., Miyazaki, J. and Smith, A.G. (2000) Quantitative expression of Oct-3/4 defines differentiation, dedifferentiation or self-renewal of ES cells. Nat. Genet. 24, 372-376
6. Zwaka, T. and Thomson, J.A. (2003) Homologous recombination in human embryonic stem cells. Nat. Biotechnol. 21, 319-321
7. Matin, M.M., Walsh, J.R., Gokhale, J., Draper, J.S., Bahrami, A.R., Morton, I., Moore, H.D. and Andrews, W. (2004) Specific knockdown of Oct4 and β2-microglobulin expression by RNA interference in human embryonic stem cells and embryonic carcinoma cells. Stem Cells 22, 659-668
8. Mitchison, T.J. (1994) Towards a pharmacological genetics. Chem. Biol. 1, 3-6
9. Schreiber, S.L. (1998) Chemical genetics resulting from a passion for synthetic organic chemistry. Bioorg. Med. Chem. 6, 1127-1152
10. Davies, S., Reddy, H., Caivano, M. and Cohen, P. (2000) Specificity and mechanism of action of some commonly used protein kinase inhibitors. Biochem. J. 351, 95-105
11. Bain, J., Plater, L., Elliott, M., Shpiro, N., Hastie, C.J., McLauchlan, H., Klevernic, I., Arthur, J.S., Alessi, D.R. and Cohen, P. (2007) The selectivity of protein kinase inhibitors: a further update. Biochem. J. 408, 297-315
12. Stelling, J., Sauer, U., Szallasi, Z., Doyle, III, F.J. and Doyle, J. (2004) Robustness of cellular functions. Cell 118, 675-685
13. Barbaric, I., Miller, G. and Dear, T.N. (2007) Appearances can be deceiving: phenotypes of knockout mice. Brief. Funct. Genomics Proteomics 6, 91-103
14. Strickland, S. and Mahdavi, V. (1978) The induction of differentiation in teratocarcinoma stem cells by retinoic acid. Cell 15, 393-403 (Pubitemid 9052408)
15. Andrews, W. (1984) Retinoic acid induces neuronal differentiation of a cloned human embryonal carcinoma cell line in vitro. Dev. Biol. 103, 285-293
16. Jakob, H., Dubois, P., Eisen, H. and Jacob, F. (1978) Effects of hexamethylenebisacetamide on the differentiation of embryonal carcinoma cells. C. R. Acad. Sci. Hebd. Seances Acad. Sci. D. 286, 109-111
17. Friend, C., Scher, W., Holland, J. and Sato, T. (1971) Hemoglobin synthesis in murine virus-induced leukemic cells in vitro: stimulation of erythroid differentiation by dimethyl sulfoxide. Proc. Natl. Acad. Sci. U.S.A. 68, 378-382
18. Watanabe, K., Ueno, M., Kamiya, D., Nishiyama, A., Matsumura, M., Wataya, T., Takahashi, J.B., Nishikawa, S., Nishikawa, S., Muguruma, K. and Sasai, Y. (2007) A ROCK inhibitor permits survival of dissociated human embryonic stem cells. Nat. Biotechnol. 25, 681-686
19. Li, X., Krawetz, R., Liu, S., Meng, G. and Rancourt, D.E. (2009) ROCK inhibitor improves survival of cryopreserved serum/feeder-free single human embryonic stem cells. Hum. Reprod. 24, 580-589
20. Placantonakis, D.G., Tomishima, M.J., Lafaille, F., Desbordes, S.C., Jia, F., Socci, N.D., Viale, A., Lee, H., Harrison, N., Tabar, V. and Studer, L. (2009) BAC transgenesis in human embryonic stem cells as a novel tool to define the human neural lineage. Stem Cells 27, 521-532
21. Desbordes, S.C., Placantonakis, D.G., Ciro, A., Socci, N.D., Lee, G., Djaballah, H. and Studer, L. (2008) High-throughput screening assay for the identification of compounds regulating self-renewal and differentiation in human embryonic stem cells. Cell Stem Cell 2, 602-612 (Pubitemid 351729221)
22. Barbaric, I., Gokhale, P.J., Jones, M., Glen, A. and Andrews, P.W. (2010) Novel regulators of stem cell fates identified by a multivariate phenotype screen of small compounds on human embryonic stem cell colonies. Stem Cell Res., doi:10.1016/j.scr.2010.04.006
23. Chen, S., Do, J.T., Zhang, Q., Yao, S., Yan, F., Peters, E.C., Scholer, H.R., Schultz, G. and Ding, S. (2006) Self-renewal of embryonic stem cells by a small molecule. Proc. Natl. Acad. Sci. U.S.A. 103, 17266-17271
24. Borowiak, M., Maehr, R., Chen, S., Chen, A.E., Tang, W., Fox, J.L., Schreiber, S.L. and Melton, D.A. (2009) Small molecules efficiently direct endodermal differentiation of mouse and human embryonic stem cells. Cell Stem Cell 4, 348-358
25. Zhu, S., Wurdak, H., Wang, J., Lyssiotis, C.A., Peters, E.C., Cho, C.Y., Wu, X. and Schultz, G. (2009) A small molecule primes embryonic stem cells for differentiation. Cell Stem Cell 4, 416-426
26. Shi, Y., Desponts, C., Do, J.T., Hahm, H.S., Scholer, H.R. and Ding, S. (2008) Induction of pluripotent stem cells from mouse embryonic fibroblasts by Oct4 and Klf4 with small-molecule compounds. Cell Stem Cell 3, 568-574
27. Huangfu, D., Osafune, K., Maehr, R., Guo, W., Eijkelenboom, A., Chen, S., Muhlestein, W. and Melton, D.A. (2008) Induction of pluripotent stem cells from primary human fibroblasts with only Oct4 and Sox2. Nat. Biotechnol. 26, 1269-1275
28. Li, W., Wei, W., Zhu, S., Zhu, J., Shi, Y., Lin, T., Hao, E., Hayek, A., Deng, H. and Ding, S. (2009) Generation of rat and human induced pluripotent stem cells by combining genetic reprogramming and chemical inhibitors. Cell Stem Cell 4, 16-19
29. Lyssiotis, C.A., Foreman, R.K., Staerk, J., Garcia, M., Mathur, D., Markoulaki, S., Hanna, J., Lairson, L.L., Charette, B.D., Bouchez, L.C. et al. (2009) Reprogramming of murine fibroblasts to induced pluripotent stem cells with chemical complementation of Klf4. Proc. Natl. Acad. Sci. U.S.A. 106, 8912-8917
30. Zhang, J.H., Chung, T.D. and Oldenburg, K.R. (1999) A simple statistical parameter for use in evaluation and validation of high throughput screening assays. J. Biomol. Screen. 4, 67-73
31. Malo, N., Hanley, J.A., Cerquozzi, S., Pelletier, J. and Nadon, R. (2006) Statistical practice in high-throughput screening data analysis. Nat. Biotechnol. 24, 167-175
32. Ding, S., Wu, T.Y., Brinker, A., Peters, E.C., Hur, W., Gray, N.S. and Schultz, G. (2003) Synthetic small molecules that control stem cell fate. Proc. Natl. Acad. Sci. U.S.A. 100, 7632-7637
33. Wu, X., Ding, S., Ding, Q., Gray, N.S. and Schultz, G. (2004) Small molecules that induce cardiomyogenesis in embryonic stem cells. J. Am. Chem. Soc. 126, 1590-1591
34. Enver, T., Pera, M., Peterson, C. and Andrews, W. (2009) Stem cell states, fates, and the rules of attraction. Cell Stem Cell 4, 387-397
35. Giuliano, K.A., DeBiasio, R.L., Dunlay, R.T., Gough, A., Volosky, J.M., Zock, J., Pavlakis, G.N. and Taylor, D.L. (1997) High-content screening: a new approach to easing key bottlenecks in the drug discovery process. J. Biomol. Screen. 3, 217-225
36. Zhang, S.C., Wernig, M., Duncan, I.D., Brustle, O. and Thomson, J.A. (2001) In vitro differentiation of transplantable neural precursors from human embryonic stem cells. Nat. Biotechnol. 19, 1129-1133
37. Loo, L.H., Wu, L.F. and Altschuler, S.J. (2007) Image-based multivariate profiling of drug responses from single cells. Nat. Methods 4, 445-453
38. Dimos, J.T., Rodolfa, K.T., Niakan, K.K., Weisenthal, L.M., Mitsumoto, H., Chung, W., Croft, G.F., Saphier, G., Leibel, R., Goland, R. et al. (2008) Induced pluripotent stem cells generated from patients with ALS can be differentiated into motor neurons. Science 321, 1218-1221
39. Park, I.H., Arora, N., Huo, H., Maherali, N., Ahfeldt, T., Shimamura, A., Lensch, M.W., Cowan, C., Hochedlinger, K. and Daley, G.Q. (2008) Disease-specific induced pluripotent stem cells. Cell 134, 877-886
40. Lee, G., Papapetrou, E., Kim, H., Chambers, S.M., Tomishima, M.J., Fasano, C.A., Ganat, Y.M., Menon, J., Shimizu, F., Viale, A. et al. (2009) Modelling pathogenesis and treatment of familial dysautonomia using patient-specific iPSCs. Nature 461, 402-406