The pitfalls of protein quantification in wastewater treatment studies

Science of the Total Environment

Volumn 408, Issue 20, 2010, Pages 4906-4909

  Avella, A C ^a   Gorner T ^a   de Donato, Ph ^a  

^a Faculté des Sciences et Technologies   (France)

Author keywords

Humic acid interference; Lowry method; Protein quantification; UV Vis spectroscopy

Indexed keywords

COMMERCIAL ASSAYS; HUMIC ACID; HUMIC SUBSTANCES; LOWRY METHOD; ORGANIC MATTER; PRINCIPAL COMPONENTS; PROTEIN QUANTIFICATION; UV-VIS SPECTROSCOPY; WASTEWATER TREATMENT PROCESS;

BIOLOGICAL MATERIALS; CHEMICALS REMOVAL (WATER TREATMENT); ORGANIC ACIDS; ULTRAVIOLET SPECTROSCOPY; WASTEWATER; WASTEWATER TREATMENT; WATER TREATMENT PLANTS;

PROTEINS;

BOVINE SERUM ALBUMIN; COPPER SULFATE; HUMIC ACID; ORGANIC MATTER; PROTEIN; HUMIC SUBSTANCE; WATER POLLUTANT;

CONCENTRATION (COMPOSITION); HUMIC ACID; HUMIC SUBSTANCE; PROTEIN; SPECTRAL ANALYSIS; WASTE TREATMENT; WASTEWATER;

ARTICLE; CALIBRATION; CLASSIC LOWRY METHOD; CONCENTRATION (PARAMETERS); CORRECTED LOWRY METHOD; METHODOLOGY; MOLECULAR INTERACTION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN QUALITY; SPECTROPHOTOMETRY; WASTE WATER; WASTE WATER MANAGEMENT; ANALYSIS; ENVIRONMENTAL MONITORING; EVALUATION STUDY; HUMIC SUBSTANCE; PROCEDURES; SEWAGE; WATER POLLUTANT;

ENVIRONMENTAL MONITORING; HUMIC SUBSTANCES; PROTEINS; WASTE DISPOSAL, FLUID; WATER POLLUTANTS, CHEMICAL;

EID: 77955921662     PISSN: 00489697     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.scitotenv.2010.05.039     Document Type: Article

References (23)

1. AOAC (Association of Official Analytical Chemists) Official Method 991.20.
2. Box J.D. Investigation of the Folin-Ciocalteau phenol reagent for the determination of polyphenolic substances in natural waters. Water Res 1983, 17:511-525.
3. Bradford M. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of Protein-Dye Binding. Anal Biochem 1976, 72(1-2):248-254.
4. Davis E.M. Protein assay: a review of common techniques. Am Biotechnol Lab 1988, 6:28-37.
5. Fan F., Zhou H., Husain H. Identification of wastewater sludge characteristics to predict critical flux for membrane bioreactor process. Water Res 2006, 40(2):205-212.
6. Flemming H.C., Wingender J. Relevance of microbial extracellular polymeric substances (EPSs) - Part I: structural and ecological aspects. Water Sci Tech 2001, 43:1-8. (6). H.C. Flemming, A. Leis (Eds.).
7. Frolund B., Griebe T., Nielsen P.H. Enzymatic activity in the activated sludge floc matrix. Appl Microbiol Biotechnol 1995, 43(4):755-761.
8. Frolund B., Palmgren R., Keiding K., Nielsen P.H. Extraction of extracellular polymers from activated sludge using a cation exchange resin. Water Res 1996, 30(8):1749-1758.
9. Jahn A., Nielsen P.H. Extraction of extracellular polymeric substances (EPS) from biofilms using a cation exchange resin. Water Sci Tech 1995, 32(8):157-164.
10. Jahn A., Nielsen P.H. Cell biomass and exopolymer composition in sewer biofilms. Water Sci Tech 1998, 31(1):17-24.
11. Kang K.H., Shin H.S., Park H. Characterization of humic substances present in landfill leachates with different landfill ages and its implications. Water Res 2002, 36(16):4023-4032.
12. Laspidou C.S., Rittmann B.E. A unified theory for extracellular polymeric substances soluble microbial products, and active and inert biomass. Water Res 2002, 36(11):2711-2720.
13. Le-Clech P., Chen V., Fane T.A.G. Fouling in membrane bioreactors used in wastewater treatment. Review. J Membr Sci 2006, 284(1-2):17-53.
14. Leenheer J.A., Nanny M.A., McIntyre C. Terpenoids as major precursors of dissolved organic matter in landfill leachates, surface water and groundwater. Environ Sci Technol 2003, 37(11):2323-2331.
15. Liu Y., Fang H. Influences of extracellular polymeric substances (EPS) on flocculation, settling and dewatering of activated sludge. Crit Rev Environ Sci Technol 2003, 33(3):237-273.
16. Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J. Protein measurement with the Folin phenol reagent. J Biol Chem 1951, 193:265-275.
17. Lyko S., Al-halbouni D., Wintgens T., Janot A., Hollender J., Dott W., et al. Polymeric compounds in activated sludge supernatant - characterisation and retention mechanisms at a full-scale municipal membrane bioreactor. Water Res 2007, 41(17):3894-3902.
18. MacCarthy P., Clapp C.E., Malcom R.L., Bloom P.R. Humic substances in soil and crop sciences: selected readings. Madison, Wisconsin, Soil Sci. Society of America 1990.
19. Peterson G.L. A simplification of the protein assay method of Lowry et al. which is more generally applicable. Anal Chem 1977, 83:346-356.
20. Peterson G.L. Review of the folin phenol protein quantification method of Lowry, Rosebrough, Farr and Randall. Anal Biochem 1979, 100:201-219.
21. Raunkjaer K., Hvitved-Jacobsen T., Nielsen P.H. Measurement of pools of protein, carbohydrate and lipid in domestic wastewater. Water Res 1994, 28(2):251-262.
22. Sutherland I.W. The biofilm matrix - an immobilized but dynamic microbial environment. Trends Microbiol 2001, 9(5):222-227.
23. Urbain V., Block J.C., Manem J. Bioflocculation in activated sludge: an analytic approach. Water Res 1993, 27(5):829-838.