Genetic variability and phospholipase production of Malassezia pachydermatis isolated from dogs with diverse grades of skin lesions

Medical Mycology

Volumn 48, Issue 6, 2010, Pages 889-892

  MacHado, Mauro L S ^a   Cafarchia, Claudia ^b   Otranto, Domenico ^b   Ferreira, Rafael R ^a   Bianchi, Simone P ^a   Latrofa, Maria S ^b   Parisi, Antonio ^c   Ferreiro, Laerte ^a  

^a FEDERAL UNIVERSITY OF RIO GRANDE DO SUL   (Brazil)

^b UNIVERSITY OF BARI   (Italy)

^c ISTITUTO ZOOPROFILATTICO SPERIMENTALE DELLA PUGLIA E DELLA BASILICATA   (Italy)

Author keywords

Dog; Malassezia pachydermatis; Molecular characterization; Phospholipase; Skin damage

Indexed keywords

CHITIN SYNTHASE; INTERNAL TRANSCRIBED SPACER 1; PHOSPHOLIPASE;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; DISEASE SEVERITY; DOG; ENZYME SYNTHESIS; FUNGAL GENETICS; FUNGUS ISOLATION; GENE LOCUS; GENE SEQUENCE; GENETIC VARIABILITY; MALASSEZIA PACHYDERMATIS; NONHUMAN; NUCLEOTIDE SEQUENCE; SKIN DEFECT;

ANIMALS; BRAZIL; CHITIN SYNTHASE; DERMATOMYCOSES; DNA, FUNGAL; DNA, RIBOSOMAL; DNA, RIBOSOMAL SPACER; DOG DISEASES; DOGS; FUNGAL PROTEINS; GENES, RRNA; GENETIC VARIATION; MALASSEZIA; PHOSPHOLIPASES; RNA, FUNGAL; RNA, RIBOSOMAL; SEQUENCE ANALYSIS, DNA; SKIN;

ANIMALIA; CANIS FAMILIARIS; MALASSEZIA PACHYDERMATIS;

EID: 77955916202     PISSN: 13693786     EISSN: 14602709     Source Type: Journal    
DOI: 10.3109/13693780903532080     Document Type: Article

References (18)

1. Guillot J, Breugnot C, De Barros M, Chermette R. Usefulness of Dixon's medium for quantitative culture of Malassezia species from canine skin. J Vet Diag Invest 1998; 10 : 382-384.
2. Coutinho SD, Paula CR. Proteinase, phospholipase, hyaluronidase and chondrointin-sulphatase production by Malassezia pachydermatis . Med Mycol 2000; 38 : 73-76.
3. Cafarchia C, Otranto D. Association between phospholipase production by Malassezia pachydermatis and skin lesions. J Clin Microbiol 2004; 42 : 4868-4869.
4. Guého E, Midgley G, Guillot J. The genus Malassezia with description of four new species. Antonie van Leeuwenhoek 1996; 69: 337-355.
5. Chen TA, Hill PB. The biology of Malassezia organisms and their ability to induce immune responses and skin disease. Vet Dermatol 2005; 16 : 4-26.
6. Ashbee HR. Update on the genus Malassezia . Med Mycol 2007; 45 : 287-303.
7. Guillot J, Guého E, Chevrier G, Chermette R. Epidemiological analysis of Malassezia pachydermatis isolates by partial sequencing of the large subunit ribosomal RNA. Res Vet Sci 1997; 62 : 22-25.
8. Aizawa T, Kano R, Nakamura Y, Watanabe S, Hasegawa A. The genetic diversity of clinical isolates of Malassezia pachydermatis from dogs and cats. Med Mycol 2001; 39 : 329-334.
9. Castellá G, Hérnandez JJ, Cabañes FJ. Genetic typing of M alassezia pachydermatis from different domestic animals. Vet Microbiol 2005; 108 : 291-296.
10. Cafarchia C, Latrofa MS, Testini G, et al . Molecular characterization of Malassezia isolates from dogs using three distinct genetic markers in nuclear DNA. Mol Cell Probes 2007; 21 : 229-238.
11. Cafarchia C, Otranto D, Campbell BE, e t al . Multilocus mutation scanning for the analysis of genetic variation within Malassezia (Basidiomycota: Malasseziales). Electrophoresis 2007; 28 : 1176-1180.
12. Cafarchia C, Gasser RB, Latrofa MS, et al . Genetic variants of Malassezia pachydermatis from canine skin: body distribution and phospholipase activity. FEMS Yeast Res 2008; 8 : 451-459.
13. Olivry T, Marsella R, Iwasaki T, e t al . Validation of CADESI-03, a severity scale for clinical trials enrolling dogs with atopic dermatitis. Vet Dermatol 2007; 18 : 78-86.
14. Xu J, Saunders CW, Hu P, et al . Dandruff-associated Malassezia genomes reveal convergent and divergent virulence traits shared with plant and human fungal pathogens. Proc Natl Acad Sci USA 2007; 104 : 18730-18735.
15. Scott DW, Miller WH, Griffi n CE. (eds). Muller and Kirk ' s Small Animal Dermatology , 5th ed. Philadelphia: WB Saunders 1995: 753-769.
16. Gaitanis G, Chasapi V, Velegraki A. Novel application of the Masson- Fontana stain for demonstrating Malassezia species melanin-like pigment production in vitro and in clinical specimens. J Clin Microbiol 2005; 43 : 4147-4151.
17. Cafarchia C, Dell'Aquila ME, Traversa D, et al . Expression of the micro-opioid receptor on Malassezia pachydermatis and its effect in modulating phospholipase production. Med Mycol 2009; 18 : 1-6.
18. Cafarchia C, Gallo S, Danesi P, et al . Assessing the relationship between Malassezia and leishmaniasis in dogs with or without skin lesions. Acta Trop 2008; 107 : 25-29.