Polymerase chain reaction in the diagnosis of onychomycosis: A large, single-institute study

British Journal of Dermatology

Volumn 163, Issue 3, 2010, Pages 511-514

  Litz, C E ^a   Cavagnolo, R Z ^a  

^a ProPath   (United States)

Author keywords

culture; onychomycosis; periodic acid Schiff stain; polymerase chain reaction; potassium hydroxide

Indexed keywords

POTASSIUM HYDROXIDE;

ANIMAL CELL; ARTICLE; CELL ISOLATION; COMPARATIVE STUDY; DERMATOPHYTE; DIAGNOSTIC TEST; FUNGUS CULTURE; HUMAN; HUMAN CELL; HUMAN EXPERIMENT; MAJOR CLINICAL STUDY; NAIL; NONHUMAN; ONYCHOMYCOSIS; PERIODIC ACID SCHIFF STAIN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL;

CULTURE MEDIA; FOOT DERMATOSES; FUNGI; HAND DERMATOSES; HUMANS; HYDROXIDES; ONYCHOMYCOSIS; PERIODIC ACID-SCHIFF REACTION; POLYMERASE CHAIN REACTION; POTASSIUM COMPOUNDS;

EID: 77955914620     PISSN: 00070963     EISSN: 13652133     Source Type: Journal    
DOI: 10.1111/j.1365-2133.2010.09852.x     Document Type: Article

References (22)

1. Ghannoum MA, Hajjeh RA, Scher R et al. A large-scale North American study of fungal isolates from nails: the frequency of onychomycosis, fungal distribution, and antifungal susceptibility patterns. J Am Acad Dermatol 2000 43:641 648.
2. Nunley KS, Cornelius L. Current management of onychomycosis. J Hand Surg Am 2008 33:1211 1214.
3. Finch JJ, Warshaw EM. Toenail onychomycosis: current and future treatment options. Dermatol Ther 2007 20:31 46.
4. Debruyne D, Coquerel A. Pharmacokinetics of antifungal agents in onychomycoses. Clin Pharmacokinet 2001 40:441 472.
5. Roberts DT, Taylor WD, Boyle J. Guidelines for treatment of onychomycosis. Br J Dermatol 2003 148:402 410.
6. Barak O, Loo DS. AN-2690, a novel antifungal for the topical treatment of onychomycosis. Curr Opin Investig Drugs 2007 8:662 668.
7. Lawry MA, Haneke E, Strobeck K et al. Methods for diagnosing onychomycosis: a comparative study and review of the literature. Arch Dermatol 2000 136:1112 1116.
8. Daniel CR 3rd., Elewski BE. The diagnosis of nail fungus infection revisited. Arch Dermatol 2000 136:1162 1164.
9. Weinberg JM, Koestenblatt EK, Tutrone WD et al. Comparison of diagnostic methods in the evaluation of onychomycosis. J Am Acad Dermatol 2003 49:193 197.
10. Reisberger EM, Abels C, Landthaler M, Szeimies RM. Histopathological diagnosis of onychomycosis by periodic acid-Schiff-stained nail clippings. Br J Dermatol 2003 148:749 754.
11. Gupta AK, Ryder JE, Summerbell RC. Onychomycosis: classification and diagnosis. J Drugs Dermatol 2004 3:51 6.
12. Panasiti V, Borroni RG, Devirgiliis V et al. Comparison of diagnostic methods in the diagnosis of dermatomycoses and onychomycoses. Mycoses 2006 49:26 9.
13. Feuilhade de Chauvin M. New diagnostic techniques. J Eur Acad Dermatol Venereol 2005 19 (Suppl. 1 20 4.
14. Cabral A, Berger TH, Middag-Broekman JH, Boon ME. Unequivocal morphological diagnosis of fungi in morphologically abnormal nails. Histopathology 2006 48:862 867.
15. Lilly KK, Koshnick RL, Grill JP et al. Cost-effectiveness of diagnostic tests for toenail onychomycosis: a repeated-measure, single-blinded, cross-sectional evaluation of 7 diagnostic tests. J Am Acad Dermatol 2006 55:620 626.
16. Litz CE, Cavagnolo RZ. Polymerase chain reaction (PCR) in the diagnosis of onychomycosis. Paper presented at the 108th American Society of Microbiology Meeting, Boston, MA, U.S.A., 2008.
17. Kano R, Nakamura Y, Watari T et al. Molecular analysis of chitin synthase 1 (CHS1) gene sequences of Trichophyton mentagrophytes complex and T. rubrum. Curr Microbiol 1998 37:236 239.
18. Kano R, Hirai A, Hasegawa A. Chitin synthase 1 gene of Arthroderma benhamiae isolates in Japan. Mycoses 2002 45:277 281.
19. Hirai A, Kano R, Nakamura Y et al. Molecular taxonomy of dermatophytes and related fungi by chitin synthase 1 (CHS1) gene sequences. Antonie Van Leeuwenhoek 2003 83:11 20.
20. Faergemann J, Baran R. Epidemiology, clinical presentation and diagnosis of onychomycosis. Br J Dermatol 2003 149 (Suppl. 65 1 4.
21. Gupta AK, Ricci MJ. Diagnosing onychomycosis. Dermatol Clin 2006 24:365 369.
22. American Medical Association. RBRVS Data Manager. Chicago, IL:AMA, 2009.