Comparison of nested PCR and real-time PCR for the detection of Toxoplasma gondii in biological samples from naturally infected cats

Research in Veterinary Science

Volumn 89, Issue 2, 2010, Pages 212-213

  Montoya, Ana ^a   Miró, Guadalupe ^a   Blanco, María Alejandra ^b   Fuentes, Isabel ^b  

^a UNIVERSIDAD COMPLUTENSE DE MADRID   (Spain)

^b INSTITUTO DE SALUD CARLOS III   (Spain)

Author keywords

Cat; Diagnosis; Nested PCR; Real time PCR; Toxoplasma gondii

Indexed keywords

GUANIDINE THIOCYANATE; PROTEINASE K; PROTOZOAL DNA; THIOCYANIC ACID DERIVATIVE; UNCLASSIFIED DRUG;

ANIMAL TISSUE; ARTICLE; BIOASSAY; CAT; CONTROLLED STUDY; DIAGNOSTIC VALUE; DNA EXTRACTION; INTERMETHOD COMPARISON; NESTED POLYMERASE CHAIN REACTION; NONHUMAN; PARASITE IDENTIFICATION; POLYMERASE CHAIN REACTION; REAL TIME POLYMERASE CHAIN REACTION; SENSITIVITY ANALYSIS; TOXOPLASMA GONDII; TOXOPLASMOSIS;

ANIMALS; CAT DISEASES; CATS; POLYMERASE CHAIN REACTION; TOXOPLASMA; TOXOPLASMOSIS, ANIMAL;

TOXOPLASMA; TOXOPLASMA GONDII;

EID: 77955896410     PISSN: 00345288     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.rvsc.2010.02.020     Document Type: Article

References (12)

1. Bastien P. Molecular diagnosis of toxoplasmosis. Transactions of the Royal Society of Tropical Medicine and Hygiene 2002, 96:205-215.
2. Burg J.L., Grober C.M., Pouletty P., Boothroyd J. Direct and sensitive detection of a pathogenic protozoan, Toxoplasma gondii, by polymerase chain reaction. Journal of Clinical Microbiology 1989, 27:1787-1792.
3. Casas I., Powell L., Klapper P.E., Cleator G.M. New method for the extraction of viral RNA and DNA from cerebrospinal fluid for use in the polymerase chain reaction assay. Journal of Virology Methods 1995, 53:25-36.
4. Costa J.M., Pautas C., Ernault P., Foulet F., Cordonnier C., Bretagne S. Real-time PCR for diagnosis and follow-up of Toxoplasma reactivation after allogeneic stem cell transplantation using fluorescence resonance energy transfer hybridation probes. Journal of Clinical Microbiology 2000, 38:2929-2932.
5. Dubey J.P., Beattie C.P. Toxoplasmosis of Animals and Man 1988, CRC Press, Boca Raton, FL, p. 140.
6. Fuentes I., Rodriguez M., Domingo C.J., del Castillo F., Juncosa T., Alvar J. Urine simple used for congenital toxoplasmosis diagnosis by PCR. Journal Clinical of Microbiology 1996, 34:2368-2371.
7. Grob U., Roggenkamp A., Janitschke K., Heesemann J. Improved sensitivity of the polymerase chain reaction for detection of Toxoplasma gondii in biological and human clinical specimens. European Journal of Clinical Microbiology and Infectious Diseases 1992, 11:33-39.
8. Lin M.H., Chen T.C., Kuo T.T., Tseng C.C., Tseng C.P. Real-time PCR for quantitative detection of Toxoplasma gondii. Journal Clinical of Microbiology 2000, 38:4121-4125.
9. Montoya A., Miró G., Mateo M., Ramírez C., Fuentes I. Detection of Toxoplasma gondii in cats by comparing bioassay in mice and polymerase chain reaction (PCR). Veterinary Parasitology 2009, 160:159-162.
10. Schatzberg S.J., Haley N.J., Barr S.C., de Lahunta A., Olby N., Munana K., Sharp N.J.H. Use of a multiplex polymerase chain reaction assay in the antemortem diagnosis of toxoplasmosis and neosporosis in the central nervous system of cats and dogs. American Journal Veterinary Research 2003, 64:1507-1513.
11. Sharma S.P., Dubey J.P. Quantitative survival of Toxoplasma gondii tachyzoites and bradyzoites in pepsin and in trypsin solutions. American Journal Veterinary Research 1981, 42:128-130.
12. Sreekumar C., Rao J.R., Mishra A.K., Ray D., Joshi P., Singh R.K. Detection of toxoplasmosis in experimentally infected goats by PCR. Veterinary Record 2004, 154:632-635.