Divergent metabolome and proteome suggest functional independence of dual phloem transport systems in cucurbits

Proceedings of the National Academy of Sciences of the United States of America

Volumn 107, Issue 30, 2010, Pages 13532-13537

  Zhang, Baichen ^a   Tolstikov, Vladimir ^b   Turnbull, Colin ^c   Hicks, Leslie M ^a   Fiehn, Oliver ^b  

^a DONALD DANFORTH PLANT SCIENCE CENTER   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c IMPERIAL COLLEGE LONDON   (United Kingdom)

Author keywords

Cucurbitaceae; Extrafascicular; Exudate; P protein; Symplastic loading

Indexed keywords

PROTEIN; PROTEOME; RNA; SUGAR; CARBOHYDRATE; PLANT EXUDATE; VEGETABLE PROTEIN;

ARTICLE; CELL LABELING; CUCUMBER; METABOLITE; METABOLOME; MICROSCOPY; NONHUMAN; PHLOEM; PHOTOSYNTHATE; PRIORITY JOURNAL; SAP FLOW; SHOOT; AMINO ACID SEQUENCE; ANATOMY AND HISTOLOGY; AUDIOVISUAL EQUIPMENT; BIOLOGICAL MODEL; CITRULLUS; CLASSIFICATION; CUCURBITACEAE; FLUORESCENCE MICROSCOPY; GENETICS; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; METABOLISM; MOLECULAR GENETICS; PLANT EXUDATE; PLANT STEM; PROCEDURES; SEQUENCE HOMOLOGY; SPECIES DIFFERENCE; SQUASH; TRANSPORT AT THE CELLULAR LEVEL;

AMINO ACID SEQUENCE; BIOLOGICAL TRANSPORT; CARBOHYDRATES; CHROMATOGRAPHY, LIQUID; CITRULLUS; CUCUMIS SATIVUS; CUCURBITA; CUCURBITACEAE; MASS SPECTROMETRY; METABOLOME; MICROSCOPY, FLUORESCENCE; MODELS, ANATOMIC; MODELS, BIOLOGICAL; MOLECULAR SEQUENCE DATA; PHLOEM; PLANT EXUDATES; PLANT PROTEINS; PLANT STEMS; PROTEOME; SEQUENCE HOMOLOGY, AMINO ACID; SPECIES SPECIFICITY;

EID: 77955827413     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.0910558107     Document Type: Article

References (43)

1. Fisher DB (2002) Long-distance transport. Biochemistry & Molecular Biology of Plants, ed Clarke AE (Wiley, Hoboken, NJ), pp 730-784.
2. Ruiz-Medrano R, Xoconostle-Cázares B, Lucas WJ (2001) The phloem as a conduit for inter-organ communication. Curr Opin Plant Biol 4:202-209.
3. van Bel AJE (2003) The phloem, a miracle of ingenuity. Plant Cell Environ 26:125-149.
4. Fisher A (1883) Das Sieboehren system von Cucurbita. The phloem system of Cucurbita. Ber Dtsch Bot Ges 1:276-279.
5. Crafts AS (1932) Phloem anatomy, exudation and transport of organic nutrients in cucurbits. Plant Physiol 7:i4-i225.
6. Crafts AS (1936) Further studies on exudation in cucurbits. Plant Physiol 11:63-79.
7. Eschrich W, Evert RF, Heyser W (1971) Proteins of the sieve tube exudate of Cucurbita maxima. Planta 100:208-221.
8. Smith LM, Sabnis DD, Johnson RPC (1987) Immunocytochemical localization of phloem lectin from Cucurbita maxima using peroxidase and colloidal-gold labels. Planta 170:461-470.
9. Clark AM, et al. (1997) Molecular characterization of a phloem-specific gene encoding the filament protein, phloem protein 1 (PP1), from Cucurbita maxima. Plant J 12: 49-61.
10. Kempers R, Prior DAM, van Bel AJE, Oparka KJ (1993) Plasmodesmata between sieve element and companion cell of extrafascicular stem phloem of Cucurbita maxima permit passage of 3-Kda fluorescent-probes. Plant J 4:567-575.
11. Lalonde S, Tegeder M, Throne-Holst M, Frommer WB, Patrick JW (2003) Phloem loading and unloading of sugars and amino acids. Plant Cell Environ 26:37-56.
12. Turgeon R, Medville R (1998) The absence of phloem loading in willow leaves. Proc Natl Acad Sci USA 95:12055-12060.
13. Turgeon R (1996) Phloem loading and plasmodesmata. Trends Plant Sci 1:418-423.
14. Schaffer AA, Pharr DM, Madore MA (1996) Cucurbits. Photoassimilate Distribution in Plants and Crops: Source-sink Relationships, ed Schaffer AA (Dekker, New York), pp 729-753.
15. Richardson PT, Baker DA, Ho LC (1982) The chemical composition of cucurbit vascular exudates. J Exp Bot 33:1239-1247.
16. Thompson GA (1999) P-Protein trafficking through plasmodesmata. Plasmodesmata: Structure, Function, Role in Cell Communication, eds van Bel AJE, van Kesteren WJP (Springer, Berlin), pp 295-314.
17. Haritatos E, Keller F, Turgeon R (1996) Raffinose oligosaccharide concentrations measured in individual cell and tissue types in Cucumis melo L leaves: Implications for phloem loading. Planta 198:614-622.
18. Fiehn O (2003) Metabolic networks of Cucurbita maxima phloem. Phytochemistry 62: 875-886.
19. Turgeon R, Wolf S (2009) Phloem transport: cellular pathways and molecular trafficking. Annu Rev Plant Biol 60:207-221.
20. Oparka KJ, Cruz SS (2000) The great escape: Phloem transport and unloading of macromolecules. Annu Rev Plant Physiol 51:323-347.
21. Thompson GA, Schulz A (1999) Macromolecular trafficking in the phloem. Trends Plant Sci 4:354-360.
22. Evert RF, Eschrich, Eichhorn SE (1973) P-protein distribution in mature sieve elements of Cucurbita maxima. Planta 109:193-210.
23. la Cour Petersen M, Hejgaard J, Thompson GA, Schulz A (2005) Cucurbit phloem serpins are graft-transmissible and appear to be resistant to turnover in the sieve element-companion cell complex. J Exp Bot 56:3111-3120.
24. Golecki B, Schulz A, Thompson GA (1999) Translocation of structural P proteins in the phloem. Plant Cell 11:127-140.
25. Crafts AS, Crisp CE (1971) Phloem Transport in Plants (Freeman, San Francisco).
26. Esau K (1965) Plant Anatomy (Wiley, New York), 2nd Ed.
27. Grignon N, Touraine B, Durand M (1989) 6(5)Carboxyfluorescein as a tracer of phloem sap translocation. Am J Bot 76:871-877.
28. Knoblauch M, van Bel AJE (1998) Sieve tubes in action. Plant Cell 10:35-50.
29. Tolstikov VV, Fiehn O (2002) Analysis of highly polar compounds of plant origin: Combination of hydrophilic interaction chromatography and electrospray ion trap mass spectrometry. Anal Biochem 301:298-307.
30. Huang S, et al. (2009) The genome of the cucumber, Cucumis sativus L. Nat Genet 41: 1275-1281.
31. Lin MK, Lee YJ, Lough TJ, Phinney BS, Lucas WJ (2009) Analysis of the pumpkin phloem proteome provides insights into angiosperm sieve tube function. Mol Cell Proteomics 8:343-356.
32. Yoo BC, Lee JY, Lucas WJ (2002) Analysis of the complexity of protein kinases within the phloem sieve tube system. Characterization of Cucurbita maxima calmodulin-like domain protein kinase 1. J Biol Chem 277:15325-15332.
33. Walz C, Juenger M, Schad M, Kehr J (2002) Evidence for the presence and activity of a complete antioxidant defence system in mature sieve tubes. Plant J 31:189-197.
34. Xoconostle-Cázares B, et al. (1999) Plant paralog to viral movement protein that potentiates transport of mRNA into the phloem. Science 283:94-98.
35. Lough TJ, Lucas WJ (2006) Integrative plant biology: Role of phloem long-distance macromolecular trafficking. Annu Rev Plant Biol 57:203-232.
36. Webb JA, Gorham PR (1964) Translocation of photosynthetically assimilated C14 in straight-necked squash. Plant Physiol 39:663-672.
37. Mitchell DE, Gadus MV, Madore MA (1992) Patterns of assimilate production and translocation in muskmelon Cucumis Melo L. I. diurnal patterns. Plant Physiol 99: 959-965.
38. Tolstikov V, Fiehn O, Tanaka N (2007) Application of liquid chromatography-mass spectrometry analysis in metabolomics. Metabolomics: Methods and Protocols, Methods in Molecular Biology (Humana, Totowa, NJ), Vol 358, pp 141-155.
39. van Bel AJE (1993) The transport phloem: Specifics of its functioning. Prog Bot 54: 134-150.
40. Turgeon R, Webb JA, Evert RF (1975) Ultrastructure of minor veins in Cucurbita pepo leaves. Protoplasma 83:217-232.
41. Chen ZH, et al. (2000) Are isocitrate lyase and phosphoenolpyruvate carboxykinase involved in gluconeogenesis during senescence of barley leaves and cucumber cotyledons? Plant Cell Physiol 41:960-967.
42. Omid A, Keilin T, Glass A, Leshkowitz D, Wolf S (2007) Characterization of phloem-sap transcription profile in melon plants. J Exp Bot 58:3645-3656.
43. Hurkman WJ, Tanaka CK (1986) Solubilization of plant membrane proteins for analysis by two-dimensional gel electrophoresis. Plant Physiol 81:802-806.