Molecular identification of helicobacter DNA in human gastric adenocarcinoma tissues using Helicobacter species-specific 16S rRNA PCR amplification and pyrosequencing analysis

Oncology Letters

Volumn 1, Issue 3, 2010, Pages 555-558

  Han, Hye Seung ^a,b   Lee, Kyung Yung ^a   Lim, So Dug ^a   Kim, Wan Seop ^a   Hwang, Tae Sook ^a  

^a Konkuk University Hospital   (South Korea)

^b Konkuk University School of Medicine   (South Korea)

Author keywords

16S rRNA; Gastric adenocarcinoma; Helicobacter; Pyrosequencing

Indexed keywords

BACTERIAL DNA; DNA; RNA 16S;

ARTICLE; BACTERIAL GENE; BACTERIUM IDENTIFICATION; CAMPYLOBACTER HYOINTESTINALIS; DNA EXTRACTION; GENE AMPLIFICATION; HELICOBACTER CINAEDI; HELICOBACTER MUSTELAE; HUMAN; HUMAN TISSUE; NONHUMAN; POLYMERASE CHAIN REACTION; PYROSEQUENCING; STOMACH ADENOCARCINOMA;

EID: 77955759885     PISSN: 17921074     EISSN: 17921082     Source Type: Journal    
DOI: 10.3892/ol_00000098     Document Type: Article

References (27)

1. Blaser MJ and Parsonnet J: Parasitism by the 'slow' bacterium Helicobacter pylori leads to altered gastric homeostasis and neoplasia. J Clin Invest 94: 4-8, 1994.
2. Weiss J, Mecca J, da Silva E and Gassner D: Comparison of PCR and other diagnostic techniques for detection of Helicobacter pylori infection in dyspeptic patients. J Clin Microbiol 32: 1663-1668, 1994.
3. Clayton CL, Kleanthous H, Coates PJ, Morgan DD and Tabaqchali S: Sensitive detection of Helicobacter pylori by using polymerase chain reaction. J Clin Microbiol 30: 192-200, 1992.
4. Engstrand L, Nguyen AM, Graham DY and el-Zaatari FA: Reverse transcription and polymerase chain reaction amplification of rRNA for detection of Helicobacter species. J Clin Microbiol 30: 2295-2301, 1992.
5. Hammar M, Tyszkiewicz T, Wadstrom T and O'Toole PW: Rapid detection of Helicobacter pylori in gastric biopsy material by polymerase chain reaction. J Clin Microbiol 30: 54-58, 1992.
6. Hjalmarsson S, Alderborn A, Fock C, Muldin I, Kling H, Uhlen M and Engstrand L: Rapid combined characterization of microorganism and host genotypes using a single technology. Helicobacter 9: 138-145, 2004.
7. Hoshina S, Kahn SM, Jiang W, et al: Direct detection and amplification of Helicobacter pylori ribosomal 16S gene segments from gastric endoscopic biopsies. Diagn Microbiol Infect Dis 13: 473-479, 1990.
8. Valentine JL, Arthur RR, Mobley HL and Dick JD: Detection of Helicobacter pylori by using the polymerase chain reaction. J Clin Microbiol 29: 689-695, 1991.
9. Van Zwet AA, Thijs JC, Kooistra-Smid AM, Schirm J and Snijder JA: Sensitivity of culture compared with that of polymerase chain reaction for detection of Helicobacter pylori from antral biopsy samples. J Clin Microbiol 31: 1918-1920, 1993.
10. Wang JT, Lin JT, Sheu JC, Yang JC, Chen DS and Wang TH: Detection of Helicobacter pylori in gastric biopsy tissue by polymerase chain reaction. Eur J Clin Microbiol Infect Dis 12: 367-371, 1993.
11. Eckloff BW, Podzorski RP, Kline BC and Cockerill FR III: A comparison of 16S ribosomal DNA sequences from five isolates of Helicobacter pylori. Int J Syst Bacteriol 44: 320-323, 1994.
12. Ho SA, Hoyle JA, Lewis FA, et al: Direct polymerase chain reaction test for detection of Helicobacter pylori in humans and animals. J Clin Microbiol 29: 2543-2549, 1991.
13. El-Omar EM, Oien K, Murray LS, et al: Increased prevalence of precancerous changes in relatives of gastric cancer patients: critical role of H. pylori. Gastroenterology 118: 22-30, 2000.
14. Eslick GD, Lim LL, Byles JE, Xia HH and Talley NJ: Association of Helicobacter pylori infection with gastric carcinoma: a meta-analysis. Am J Gastroenterol 94: 2373-2379, 1999.
15. Limburg P, Qiao Y, Mark S, et al: Helicobacter pylori seropositivity and subsite-specific gastric cancer risks in Linxian, China. J Natl Cancer Inst 93: 226-233, 2001.
16. Uemura N, Okamoto S, Yamamoto S, et al: Helicobacter pylori infection and the development of gastric cancer. N Engl J Med 345: 784-789, 2001.
17. Kim N, Park RY, Cho SI, et al: Helicobacter pylori infection and development of gastric cancer in Korea: long-term follow-up. J Clin Gastroenterol 42: 448-454, 2008.
18. Dicksved J, Lindberg M, Rosenquist M, Enroth H, Jansson JK and Engstrand L: Molecular characterization of the stomach microbiota in patients with gastric cancer and in controls. J Med Microbiol 58: 509-516, 2009.
19. Pena JA, McNeil K, Fox JG and Versalovic J: Molecular evidence of Helicobacter cinaedi organisms in human gastric biopsy specimens. J Clin Microbiol 40: 1511-1513, 2002.
20. Quinn TC, Goodell SE, Fennell C, Wang SP, Schuffler MD, Holmes KK and Stamm WE: Infections with Campylobacter jejuni and Campylobacter-like organisms in homosexual men. Ann Intern Med 101: 187-192, 1984.
21. Burman WJ, Cohn DL, Reves RR and Wilson ML: Multifocal cellulitis and monoarticular arthritis as manifestations of Helicobacter cinaedi bacteremia. Clin Infect Dis 20: 564-570, 1995.
22. Gebhart CJ, Ward GE, Chang K and Kurtz HJ: Campylobacter hyointestinalis (new species) isolated from swine with lesions of proliferative enteritis. Am J Vet Res 44: 361-367, 1983.
23. Lastovica AJ: Campylobacter/Helicobacter bacteremia in Cape Town, South Africa, 1977-1995. In: Campylobacters, Helicobacters and Related Organisms. Newell DG, Ketley JM and Feldman RA (eds). Plenum Press, New York, pp475-479, 1996.
24. Fox JG, Edrise BM, Cabot EB, Beaucage C, Murphy JC and Prostak KS: Campylobacter-like organisms isolated from gastric mucosa of ferrets. Am J Vet Res 47: 236-239, 1986.
25. Yu J, Russell RM, Salomon RN, Murphy JC, Palley LS and Fox JG: Effect of Helicobacter mustelae infection on ferret gastric epithelial cell proliferation. Carcinogenesis 16: 1927-1931, 1995.
26. Fox JG, Correa P, Taylor NS, Lee A, Otto G, Murphy JC and Rose R: Helicobacter mustelae associated gastritis in ferrets: an animal model of Helicobacter pylori gastritis in humans. Gastroenterology 99: 352-361, 1990.
27. Fox JG, Dangler CA, Sager W, Borkowski R and Gliatto JM: Helicobacter mustelae-associated gastric adenocarcinoma in ferrets (Mustela putorius furo). Vet Pathol 34: 225-229, 1997.