Functional role of Thr-312 and Thr-315 in the proton-transfer pathway in ba 3 cytochrome c oxidase from thermus thermophilus

Biochemistry

Volumn 49, Issue 33, 2010, Pages 7033-7039

  Smirnova, Irina ^a,d   Reimann, Joachim ^a   Von Ballmoos, Christoph ^a   Chang, Hsin Yang ^b   Gennis, Robert B ^b   Fee, James A ^c   Brzezinski, Peter ^a   Ädelroth, Pia ^a  

^a STOCKHOLM UNIVERSITY   (Sweden)

^b UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

^c SCRIPPS RESEARCH INSTITUTE   (United States)

^d MOSCOW STATE UNIVERSITY   (Russian Federation)

Author keywords

[No Author keywords available]

Indexed keywords

ACTIVE SITE; CYTOCHROME C OXIDASE; HEME-COPPER OXIDASE; LOW DEGREE; PROTON UPTAKE; SEQUENCE HOMOLOGY; THERMUS THERMOPHILUS; TIME CONSTANTS; TIME-RESOLVED; WILD TYPES;

PORPHYRINS;

PROTONS;

CYTOCHROME C OXIDASE; THREONINE; CARBON MONOXIDE; OXYGEN; PROTON;

ABSORPTION; ARTICLE; ENZYME ACTIVE SITE; NONHUMAN; OXIDATION; PRIORITY JOURNAL; PROTON TRANSPORT; REDUCTION; THERMUS THERMOPHILUS; CHEMICAL STRUCTURE; ENZYMOLOGY; GENETICS; METABOLISM; OXIDATION REDUCTION REACTION; POINT MUTATION;

CARBON MONOXIDE; ELECTRON TRANSPORT COMPLEX IV; MODELS, MOLECULAR; OXIDATION-REDUCTION; OXYGEN; POINT MUTATION; PROTONS; THERMUS THERMOPHILUS; THREONINE;

THERMUS THERMOPHILUS;

EID: 77955692484     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi100749p     Document Type: Article

References (25)

1. 3-type cytochrome c oxidase from Thermus thermophilus FEBS Lett. 434, 17-22
2. Pereira, M. M., Santana, M., and Teixeira, M. (2001) A novel scenario for the evolution of haem-copper oxygen reductases Biochim. Biophys. Acta 1505, 185-208
3. 3-cytochrome c oxidase from Thermus thermophilus EMBO J. 19, 1766-1776
4. Wraight, C. A. (2006) Chance and design: Proton transfer in water, channels and bioenergetic proteins Biochim. Biophys. Acta 1757, 886-912
5. Brzezinski, P. and Ädelroth, P. (1998) Pathways of proton transfer in cytochrome c oxidase J. Bioenerg. Biomembr. 30, 99-107
6. Konstantinov, A. A., Siletsky, S., Mitchell, D., Kaulen, A., and Gennis, R. B. (1997) The roles of the two proton input channels in cytochrome c oxidase from Rhodobacter sphaeroides probed by the effects of site-directed mutations on time-resolved electrogenic intraprotein proton transfer Proc. Natl. Acad. Sci. U.S.A. 94, 9085-9090
7. 3 from Thermus thermophilus HB8 Protein Expression Purif. 40, 299-318
8. 3 oxygen reductase from Thermus thermophilus uses a single input channel for proton delivery to the active site and for proton pumping Proc. Natl. Acad. Sci. U.S.A. 106, 16169-16173
9. 3 oxidase from Thermus thermophilus Biochim. Biophys. Acta 1767, 1383-1392
10. 3 oxidase from Thermus thermophilus J. Bioenerg. Biomembr. 40, 281-287
11. Fee, J. A., Chen, Y., Todaro, T. R., Bren, K. L., Patel, K. M., Hill, M. G., Gomez-Moran, E., Loehr, T. M., Ai, J., Thöny-Meyer, L., Williams, P. A., Stura, E., Sridhar, V., and McRee, D. E. (2000) Integrity of Thermus thermophilus cytochrome c552 synthesized by Escherichia coli cells expressing the host-specific cytochrome c maturation genes, ccmABCDEFGH: Biochemical, spectral, and structural characterization of the recombinant protein Protein Sci. 9, 2074-2084
12. Brändén, M., Sigurdson, H., Namslauer, A., Gennis, R. B., Ädelroth, P., and Brzezinski, P. (2001) On the role of the K-proton transfer pathway in cytochrome c oxidase Proc. Natl. Acad. Sci. U.S.A. 98, 5013-5018
13. 3 oxidase from Thermus thermophilus: Effect of temperature Biochemistry 38, 1057-1065
14. 3: A second terminal oxidase from the extreme thermophile Thermus thermophilus Proc. Natl. Acad. Sci. U.S.A. 85, 5779-5783
15. Morgan, J. E., Verkhovsky, M. I., and Wikström, M. (1996) Observation and assignment of peroxy and ferryl intermediates in the reduction of dioxygen to water by cytochrome c oxidase Biochemistry 35, 12235-12240
16. 3 oxidase from Thermus thermophilus: Structure of the reduced form of the enzyme Biochemistry 48, 820-826
17. Wikström, M., Jasaitis, A., Backgren, C., Puustinen, A., and Verkhovsky, M. I. (2000) The role of the D-and K-pathways of proton transfer in the function of the haem-copper oxidases Biochim. Biophys. Acta 1459, 514-520
18. Ädelroth, P., Gennis, R. B., and Brzezinski, P. (1998) Role of the pathway through K(I-362) in proton transfer in cytochrome c oxidase from R. sphaeroides Biochemistry 37, 2470-2476
19. Hallén, S. and Nilsson, T. (1992) Proton transfer during the reaction between fully reduced cytochrome c oxidase and dioxygen: pH and deuterium isotope effects Biochemistry 31, 11853-11859
20. Karpefors, M., Ädelroth, P., Namslauer, A., Zhen, Y., and Brzezinski, P. (2000) Formation of the "peroxy" intermediate in cytochrome c oxidase is associated with internal proton/hydrogen transfer Biochemistry 39, 14664-14669
21. Proshlyakov, D. A., Pressler, M. A., and Babcock, G. T. (1998) Dioxygen activation and bond cleavage by mixed-valence cytochrome c oxidase Proc. Natl. Acad. Sci. U.S.A. 95, 8020-8025
22. Gorbikova, E. A., Belevich, I., Wikström, M., and Verkhovsky, M. I. (2008) The proton donor for O-O bond scission by cytochrome c oxidase Proc. Natl. Acad. Sci. U.S.A. 105, 10733-10737
23. Smirnova, I. A., Ädelroth, P., Gennis, R. B., and Brzezinski, P. (1999) Aspartate-132 in cytochrome c oxidase from Rhodobacter sphaeroides is involved in a two-step proton transfer during oxo-ferryl formation Biochemistry 38, 6826-6833
24. Faxén, K., Gilderson, G., Ädelroth, P., and Brzezinski, P. (2005) A mechanistic principle for proton pumping by cytochrome c oxidase Nature 437, 286-289
25. Verkhovsky, M. I., Morgan, J. E., and Wikström, M. (1995) Control of electron delivery to the oxygen reduction site of cytochrome c oxidase: A role for protons Biochemistry 34, 7483-7491