Polysaccharides PS-G and protein LZ-8 from Reishi (Ganoderma lucidum) exhibit diverse functions in regulating murine macrophages and T lymphocytes

Journal of Agricultural and Food Chemistry

Volumn 58, Issue 15, 2010, Pages 8535-8544

  Yeh, Chen Hao ^a   Chen, Hsiao Chin ^a   Yang, Jeng Je ^a   Chuang, Wen I ^a   Sheu, Fuu ^a  

^a NATIONAL TAIWAN UNIVERSITY   (Taiwan)

Author keywords

Ganoderma lucidum; immune modulation; Ling Zhi 8 (LZ 8); polysaccharide; PS G; TLR4

Indexed keywords

GANODERMA LUCIDUM; IMMUNE MODULATION; LING ZHI-8 (LZ-8); PS-G; TLR4;

CHEMICAL ACTIVATION; CHEMICAL DETECTION; MONOCLONAL ANTIBODIES; POLYSACCHARIDES; PROTEINS; YEAST;

MACROPHAGES;

CYTOKINE; FUNGAL PROTEIN; IMMUNOLOGIC FACTOR; LZ 8 PROTEIN, GANODERMA LUCIDUM; LZ-8 PROTEIN, GANODERMA LUCIDUM; POLYSACCHARIDE;

ANIMAL; ARTICLE; C57BL MOUSE; CELL CULTURE; CHEMISTRY; DRUG EFFECT; GANODERMA LUCIDUM; IMMUNOLOGY; LYMPHOCYTE ACTIVATION; MACROPHAGE; MACROPHAGE ACTIVATION; MOUSE; MOUSE MUTANT; T LYMPHOCYTE;

ANIMALS; CELLS, CULTURED; CYTOKINES; FUNGAL PROTEINS; IMMUNOLOGIC FACTORS; LYMPHOCYTE ACTIVATION; MACROPHAGE ACTIVATION; MACROPHAGES; MICE; MICE, INBRED C57BL; MICE, KNOCKOUT; POLYSACCHARIDES; REISHI; T-LYMPHOCYTES;

GANODERMA LUCIDUM; MURINAE; MUS; SACCHAROMYCES CEREVISIAE;

EID: 77955677588     PISSN: 00218561     EISSN: 15205118     Source Type: Journal    
DOI: 10.1021/jf100914m     Document Type: Article

References (36)

1. Lin, Z. B.; Zhang, H. N. Anti-tumor and immunoregulatory activities of Ganoderma lucidum and its possible mechanisms Acta Pharmacol. Sin. 2004, 25 (11) 1387-95
2. Wang, S. Y.; Hsu, M. L.; Hsu, H. C.; Tzeng, C. H.; Lee, S. S.; Shiao, M. S.; Ho, C. K. The anti-tumor effect of Ganoderma lucidum is mediated by cytokines released from activated macrophages and T lymphocytes Int. J. Cancer 1997, 70 (6) 699-705
3. Eo, S. K.; Kim, Y. S.; Lee, C. K.; Han, S. S. Possible mode of antiviral activity of acidic protein bound polysaccharide isolated from Ganoderma lucidum on herpes simplex viruses J. Ethnopharmacol. 2000, 72 (3) 475-81
4. Sun, J.; He, H.; Xie, B. J. Novel antioxidant peptides from fermented mushroom Ganoderma lucidum J. Agric. Food Chem. 2004, 52 (21) 6646-52
5. Chien, C. M.; Cheng, J. L.; Chang, W. T.; Tien, M. H.; Tsao, C. M.; Chang, Y. H.; Chang, H. Y.; Hsieh, J. F.; Wong, C. H.; Chen, S. T. Polysaccharides of Ganoderma lucidum alter cell immunophenotypic expression and enhance CD56+ NK-cell cytotoxicity in cord blood Bioorg. Med. Chem. 2004, 12 (21) 5603-9
6. Wang, Y. Y.; Khoo, K. H.; Chen, S. T.; Lin, C. C.; Wong, C. H.; Lin, C. H. Studies on the immuno-modulating and antitumor activities of Ganoderma lucidum (Reishi) polysaccharides: functional and proteomic analyses of a fucose-containing glycoprotein fraction responsible for the activities Bioorg. Med. Chem. 2002, 10 (4) 1057-62
7. Hsu, M. J.; Lee, S. S.; Lin, W. W. Polysaccharide purified from Ganoderma lucidum inhibits spontaneous and Fas-mediated apoptosis in human neutrophils through activation of the phosphatidylinositol 3 kinase/Akt signaling pathway J. Leukocyte Biol. 2002, 72 (1) 207-16
8. Lin, K. I.; Kao, Y. Y.; Kuo, H. K.; Yang, W. B.; Chou, A.; Lin, H. H.; Yu, A. L.; Wong, C. H. Reishi polysaccharides induce immunoglobulin production through the TLR4/TLR2-mediated induction of transcription factor Blimp-1 J. Biol. Chem. 2006, 281 (34) 24111-23
9. Lin, Y. L.; Liang, Y. C.; Lee, S. S.; Chiang, B. L. Polysaccharide purified from Ganoderma lucidum induced activation and maturation of human monocyte-derived dendritic cells by the NF-kappaB and p38 mitogen-activated protein kinase pathways J. Leukocyte Biol. 2005, 78 (2) 533-43
10. Hsu, H. Y.; Hua, K. F.; Lin, C. C.; Lin, C. H.; Hsu, J.; Wong, C. H. Extract of Reishi polysaccharides induces cytokine expression via TLR4-modulated protein kinase signaling pathways J. Immunol. 2004, 173 (10) 5989-99
11. Kino, K.; Yamashita, A.; Yamaoka, K.; Watanabe, J.; Tanaka, S.; Ko, K.; Shimizu, K.; Tsunoo, H. Isolation and characterization of a new immunomodulatory protein, ling zhi-8 (LZ-8), from Ganoderma lucidium J. Biol. Chem. 1989, 264 (1) 472-8
12. Tanaka, S.; Ko, K.; Kino, K.; Tsuchiya, K.; Yamashita, A.; Murasugi, A.; Sakuma, S.; Tsunoo, H. Complete amino acid sequence of an immunomodulatory protein, ling zhi-8 (LZ-8). An immunomodulator from a fungus, Ganoderma lucidium, having similarity to immunoglobulin variable regions J. Biol. Chem. 1989, 264 (28) 16372-7
13. Tong, M. H.; Chien, P. J.; Chang, H. H.; Tsai, M. J.; Sheu, F. High processing tolerances of immunomodulatory proteins in Enoki and Reishi mushrooms J. Agric. Food Chem. 2008, 56 (9) 3160-6
14. Huang, L.; Sun, F.; Liang, C. Y.; He, Y. X.; Bao, R.; Liu, L. X.; Zhou, C. Z. Crystal structure of LZ-8 from the medicinal fungus Ganoderma lucidium Proteins: Struct., Funct., Bioinf. 2009, 75 (2) 524-527
15. Haak-Frendscho, M.; Kino, K.; Sone, T.; Jardieu, P. Ling Zhi-8: a novel T cell mitogen induces cytokine production and upregulation of ICAM-1 expression Cell Immunol. 1993, 150 (1) 101-13
16. van der Hem, L. G.; van der Vliet, J. A.; Bocken, C. F.; Kino, K.; Hoitsma, A. J.; Tax, W. J. Ling Zhi-8: studies of a new immunomodulating agent Transplantation 1995, 60 (5) 438-43
17. Hsu, H. Y.; Hua, K. F.; Wu, W. C.; Hsu, J.; Weng, S. T.; Lin, T. L.; Liu, C. Y.; Hseu, R. S.; Huang, C. T. Reishi immuno-modulation protein induces interleukin-2 expression via protein kinase-dependent signaling pathways within human T cells J. Cell Physiol. 2008, 215 (1) 15-26
18. Lin, Y. L.; Liang, Y. C.; Tseng, Y. S.; Huang, H. Y.; Chou, S. Y.; Hseu, R. S.; Huang, C. T.; Chiang, B. L. An immunomodulatory protein, Ling Zhi-8, induced activation and maturation of human monocyte-derived dendritic cells by the NF-kappaB and MAPK pathways J. Leukocyte Biol. 2009, 86 (4) 877-89
19. Cao, L. Z.; Lin, Z. B. Regulation on maturation and function of dendritic cells by Ganoderma lucidum polysaccharides Immunol. Lett. 2002, 83 (3) 163-9
20. Li, Z.; Liu, J.; Zhao, Y. Possible mechanism underlying the antiherpetic activity of a proteoglycan isolated from the mycelia of Ganoderma lucidum in vitro J. Biochem. Mol. Biol. 2005, 38 (1) 34-40
21. Ou, K.; Liu, Y.; Zhang, L.; Yang, X.; Huang, Z.; Nout, M. J.; Liang, J. Effect of Neutrase, alcalase, and papain hydrolysis of whey protein concentrates on iron uptake by Caco-2 cells J. Agric. Food Chem. 2010, 58 (8) 4894-900
22. Chang, H. H.; Yeh, C. H.; Sheu, F. A novel immunomodulatory protein from Poria cocos induces Toll-like receptor 4-dependent activation within mouse peritoneal macrophages J. Agric. Food Chem. 2009, 57 (14) 6129-39
23. Mantovani, A.; Sica, A.; Sozzani, S.; Allavena, P.; Vecchi, A.; Locati, M. The chemokine system in diverse forms of macrophage activation and polarization Trends Immunol. 2004, 25 (12) 677-86
24. Xue, Q.; Ding, Y.; Shang, C.; Jiang, C.; Zhao, M. Functional expression of LZ-8, a fungal immunomodulatory protein from Ganoderma lucidium in Pichia pastoris J. Gen. Appl. Microbiol. 2008, 54 (6) 393-8
25. Ko, J. L.; Lin, S. J.; Hsu, C. I.; Kao, C. L.; Lin, J. Y. Molecular cloning and expression of a fungal immunomodulatory protein, FIP-fve, from Flammulina velutipes J. Formosan Med. Assoc. 1997, 96 (7) 517-24
26. Lin, W. H.; Hung, C. H.; Hsu, C. I.; Lin, J. Y. Dimerization of the N-terminal amphipathic alpha-helix domain of the fungal immunomodulatory protein from Ganoderma tsugae (Fip-gts) defined by a yeast two-hybrid system and site-directed mutagenesis J. Biol. Chem. 1997, 272 (32) 20044-8
27. Yeh, C. M.; Yeh, C. K.; Hsu, X. Y.; Luo, Q. M.; Lin, M. Y. Extracellular expression of a functional recombinant Ganoderma lucidium immunomodulatory protein by Bacillus subtilis and Lactococcus lactis Appl. Environ. Microbiol. 2008, 74 (4) 1039-49
28. Miyasaka, N.; Inoue, H.; Totsuka, T.; Koike, R.; Kino, K.; Tsunoo, H. An immunomodulatory protein, Ling Zhi-8, facilitates cellular interaction through modulation of adhesion molecules Biochem. Biophys. Res. Commun. 1992, 186 (1) 385-90
29. Doco, T.; Carcano, D.; Ramos, P.; Loones, A.; Fournet, B. Rapid isolation and estimation of polysaccharide from fermented skim milk with Streptococcus salivarius subsp. thermophilus by coupled anion exchange and gel-permeation high-performance liquid chromatography J. Dairy Res. 1991, 5, 147-50
30. Beri, R. G.; Rollings, J. E. A technique for the rapid removal of proteins from cell extracts containing polysaccharides Biotechnol. Tech. 1995, 9, 783-786
31. Wang, X.; Yuan, Y.; Wang, K.; Zhang, D.; Yang, Z.; Xu, P. Deproteinization of gellan gum produced by Sphingomonas paucimobilis ATCC 31461 J. Biotechnol. 2007, 128 (2) 403-7
32. Oliveira, R.; Marques, F.; Azeredo, J. Purification of polysaccharides from a biofilm matrix by selective precipitation of proteins Biotechnol. Tech. 1999, 13, 391-393
33. Taylor, P. R.; Martinez-Pomares, L.; Stacey, M.; Lin, H. H.; Brown, G. D.; Gordon, S. Macrophage receptors and immune recognition Annu. Rev. Immunol. 2005, 23, 901-44
34. Grewal, I. S.; Flavell, R. A. CD40 and CD154 in cell-mediated immunity Annu. Rev. Immunol. 1998, 16, 111-35
35. Graham, V. A.; Marzo, A. L.; Tough, D. F. A role for CD44 in T cell development and function during direct competition between CD44+ and CD44-cells Eur. J. Immunol. 2007, 37 (4) 925-34
36. Kappes, D. J.; He, X. Role of the transcription factor Th-POK in CD4:CD8 lineage commitment Immunol. Rev. 2006, 209, 237-52