Purification and kinetic characterization of the liverwort Pallavicinia lyelli (Hook.) S. Gray. cytosolic ascorbate peroxidase

Plant Physiology and Biochemistry

Volumn 48, Issue 9, 2010, Pages 758-763

  Sajitha Rajan, S ^a   Murugan, K ^a  

^a DEPARTMENT OF CHEMISTRY   (India)

Author keywords

Ascorbate peroxidase; Characterization; Hydrogen peroxide; Kinetics; Pallavicinia lyelli; Purification

Indexed keywords

PALLAVICINIA;

EID: 77955660230     PISSN: 09819428     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.plaphy.2010.06.004     Document Type: Article

References (22)

1. Arnon D.I. Copper enzymes in the isolated chloroplasts, polyphenol oxidase in Beta vulgaris. Plant Physiol. 1949, 24:1-15.
2. Caldwell C.R., Turano F.J., McMahon M.B. Identification of two cytosolic ascorbate peroxidase cDNAs from soybean leaves and characterization of their products by functional expression in E. coli. Planta 1998, 204:120-126.
3. Chen G.X., Asada K. Ascorbate peroxidase in tea leaves: occurrence of two isozymes and the differences in their enzymatic and molecular properties. Plant Cell Physiol. 1989, 30:987-998.
4. Dalton D.A., Hanus F.J., Russell S.A., Evans H.J. Purification, properties, and distribution of ascorbate peroxidase in legume root nodules. Plant Physiol. 1987, 83:789-794.
5. Gillham D.J., Dodge A.D. Chloroplast superoxide and hydrogen peroxide scavenging systems from pea leaves: seasonal variation. Plant Sci. 1987, 50:105-109.
6. Hossain M.A., Nakano Y., Asada K. Monodehydroascorbate reductase in spinach chloroplasts and its participation in regeneration of ascorbate for scavenging hydrogen peroxide. Plant Cell Physiol. 1984, 25:385-395.
7. Ishikawa T., Takeda T., Kohno H., Shigeoka S. Molecular characterization of Euglena ascorbate peroxidase using monoclonal antibody. Biochim. Biophys. Acta 1996, 1290:69-75.
8. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227:680-685.
9. Lee D.H., Lee C.B. Chilling stress-induced changes of antioxidant enzymes in the leaves of cucumber: in gel enzyme activity assays. Plant Sci. 2000, 159:75-85.
10. Lineweaver H., Burk D. The determination of enzyme dissociation constant. J. Am. Chem. Soc. 1934, 56:658-661.
11. Mittler R., Tel-Or E. Oxidative stress responses and shock proteins in the unicellular cyanobacterium Synechococcus R2 (PCC-7942). Arch. Microbiol. 1991, 155:125-131.
12. Mittler R., Zilinskas B.A. Purification and characterization of pea cytosolic ascorbate peroxidase. Plant Physiol. 1991, 97:962-968.
13. Mittler R., Zilinskas B.A. Regulation of pea cytosolic ascorbate peroxidase and other anioxidant enzymes during the progression of drought stress and following recovery from drought. Plant Physiol. 1991, 97:962-968.
14. Miyake C., Cao W.H., Asada K. Purification and molecular properties of the thylakoid-bound ascorbate peroxidase in spinach chloroplasts. Plant Cell Physiol. 1993, 34:881-889.
15. Nakano Y., Asada K. Purification of ascorbate peroxidase in spinach chloroplasts; its inactivation in ascorbate-depleted medium and reactivation by monodehydroascorbate radical. Plant Cell Physiol. 1987, 28:131-140.
16. Nishikawa F., Kato M., Wang R., Hyodo H., Ikoma Y., Sugiura M., Yano M. Two APX from broccoli: identification, expression and characterization of their recombinant proteins. Postharvest Biol. Technol. 2003, 27:147-156.
17. Orozco E.M., Mullet J.E., Hundley-Bodoim L., Chua N.H. In vitro transcription of chloroplast protein genes. Meth. Enzymol. 1986, 118:232-253.
18. Sano S., Ueda M., Kitajima S., Takeda T., Shigeoka S., Kurano N., Miyachi S., Miyake C., Yokota A. Characterization of ascorbate peroxidsae from unicellular red alga, Galdieria partita. Plant Cell Physiol. 2001, 42(4):433-440.
19. Shigeoka S., Nakano Y., Kitaoka S. Purification and some properties of L-ascorbic acid-specific peroxidase in Euglena gracilis. Arch. Biochem. Biophys. 1980, 201:121-127.
20. Shigeoka S., Yasumoto R., Onishi T., Nakano Y., Kitaoka S. Properties of monodchydroascorbate rcductase and dehydroascorbate reductase and their participation in the regeneration of ascorbate in Euglena gracilis. J. Gen. Microbiol. 1987, 133:227-232.
21. Takeda T., Shigeoka S., Hirayama O., Mitsnnaga T. The presence of enzymes related to glutathione metabolism and oxygen metabolism in Chlamydomonas reinhardtii. Biosci. Biotechnol. Biochem. 1992, 56:1662-1663.
22. Welinder K.G. Superfamily of plant, fungal and bacterial peroxidases. Curr. Opin. Struct. Biol. 1992, 2:388-393.