Multiplexed combinatorial tetramer staining in a mouse model of virus infection

Journal of Immunological Methods

Volumn 360, Issue 1-2, 2010, Pages 157-161

  Cukalac, Tania ^a   Valkenburg, Sophie A ^a   La Gruta, Nicole L ^a   Turner, Stephen J ^a   Doherty, Peter C ^a,b   Kedzierska, Katherine ^a  

^a UNIVERSITY OF MELBOURNE   (Australia)

^b ST JUDE CHILDREN S RESEARCH HOSPITAL   (United States)

Author keywords

Cytotoxic T cells; Mouse B6 model; PMHC tetramers; T cells; Viral infection

Indexed keywords

MAJOR HISTOCOMPATIBILITY ANTIGEN CLASS 1; TETRAMER; EPITOPE; FLUORESCENT DYE; HLA ANTIGEN CLASS 1; PEPTIDE FRAGMENT; VIRUS PROTEIN;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CD8+ T LYMPHOCYTE; CELL SPECIFICITY; FEMALE; FLOW CYTOMETRY; INFLUENZA; LEUKOCYTE; MOUSE; NONHUMAN; PRIORITY JOURNAL; SPLEEN CELL; ANIMAL; C57BL MOUSE; CELL CULTURE; CELL SEPARATION; COMBINATORIAL CHEMISTRY; EPITOPE MAPPING; IMMUNOLOGY; INFLUENZA VIRUS A; METABOLISM; ORTHOMYXOVIRIDAE INFECTIONS; PATHOGENICITY; PATHOLOGY; PROTEIN MULTIMERIZATION;

ANIMALS; CD8-POSITIVE T-LYMPHOCYTES; CELL SEPARATION; CELLS, CULTURED; COMBINATORIAL CHEMISTRY TECHNIQUES; EPITOPE MAPPING; EPITOPES, T-LYMPHOCYTE; FEMALE; FLOW CYTOMETRY; FLUORESCENT DYES; HISTOCOMPATIBILITY ANTIGENS CLASS I; INFLUENZA A VIRUS; MICE; MICE, INBRED C57BL; ORTHOMYXOVIRIDAE INFECTIONS; PEPTIDE FRAGMENTS; PROTEIN MULTIMERIZATION; VIRAL PROTEINS;

ANIMALIA; MUS; ORTHOMYXOVIRIDAE;

EID: 77955657270     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2010.06.003     Document Type: Article

References (9)

1. Altman J.D., Moss P.A., Goulder P.J., Barouch D.H., McHeyzer-Williams M.G., Bell J.I., McMichael A.J., Davis M.M. Phenotypic analysis of antigen-specific T lymphocytes. Science 1996, 274:94.
2. Doherty P.C., Christensen J.P. Accessing complexity: the dynamics of virus-specific T cell responses. Annu. Rev. Immunol. 2000, 18:561.
3. Doherty P.C., Turner S.J., Webby R.G., Thomas P.G. Influenza and the challenge for immunology. Nat. Immunol. 2006, 7:449.
4. Hadrup S.R., Bakker A.H., Shu C.J., Andersen R.S., van Veluw J., Hombrink P., Castermans E., Thor Straten P., Blank C., Haanen J.B., Heemskerk M.H., Schumacher T.N. Parallel detection of antigen-specific T-cell responses by multidimensional encoding of MHC multimers. Nat. Meth. 2009, 6:520.
5. + memory T cells. Proc. Natl. Acad. Sci. U. S. A. 2006, 103:9184.
6. Kedzierska K., Stambas J., Doherty P.C. Finding multiple needles in one immune haystack. Nat. Meth. 2009, 6:489.
7. La Gruta N., Rothwell W., Cukalac T., Swan N., Valkenburg S., Kedzierska K., Thomas P., Doherty P., Turner S. Primary CTL response magnitude in mice is determined by the extent of naïve T cell recruitment and subsequent clonal expansion. J. Clin. Invest. 2010, 120:1885.
8. Newell E.W., Klein L.O., Yu W., Davis M.M. Simultaneous detection of many T-cell specificities using combinatorial tetramer staining. Nat. Meth. 2009, 6:497.
9. Yewdell J.W., Bennink J.R. Immunodominance in major histocompatibility complex class I-restricted T lymphocyte responses. Annu. Rev. Immunol. 1999, 17:51.