A hybrid promoter-containing vector for direct cloning and enhanced expression of PCR-amplified ORFs in mammalian cells

Molecular Biology Reports

Volumn 37, Issue 6, 2010, Pages 2757-2765

  You, Lei Ming ^a,b   Luo, Jun ^a   Wang, Ai Ping ^b   Zhang, Gai Ping ^a   Weng, Hai Bo ^b   Guo, Ya Nan ^b   Liu, Yun Chao ^a   Li, Qiao Mu ^b   Teng, Man ^a  

^a INSTITUTE OF PLANT PROTECTION   (China)

^b ZHENGZHOU UNIVERSITY   (China)

Author keywords

Expression vector; Hybrid promoter; TA cloning

Indexed keywords

BETA ACTIN; BETA GALACTOSIDASE; BETA GLOBULIN; GREEN FLUORESCENT PROTEIN; VIRUS VECTOR; FC RECEPTOR;

ANIMAL CELL; ARTICLE; CYTOMEGALOVIRUS; DNA HYBRIDIZATION; DNA SEQUENCE; EXPRESSION VECTOR; FLOW CYTOMETRY; GENE AMPLIFICATION; GENE EXPRESSION; HUMAN; HUMAN CELL; MAMMAL CELL; MARKER GENE; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; POLYMERASE CHAIN REACTION; PROMOTER REGION; TRANSGENE; ANIMAL; CELL LINE; CYTOLOGY; ERYTHROCYTE; GENE VECTOR; GENETIC TRANSFECTION; GENETICS; INTRON; METABOLISM; MOLECULAR GENETICS; MOUSE; PLASMID; PROCEDURES;

ANIMALS; BASE SEQUENCE; CELL LINE; CLONING, MOLECULAR; ERYTHROCYTES; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; HUMANS; INTRONS; MICE; MOLECULAR SEQUENCE DATA; OPEN READING FRAMES; PLASMIDS; POLYMERASE CHAIN REACTION; PROMOTER REGIONS, GENETIC; RECEPTORS, IGG; TRANSFECTION;

MAMMALIA;

EID: 77955641226     PISSN: 03014851     EISSN: 15734978     Source Type: Journal    
DOI: 10.1007/s11033-009-9814-x     Document Type: Article

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