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Volumn 21, Issue 2, 2010, Pages 97-102

Optimization of protocol for sequencing of difficult templates

Author keywords

Additives; Complex DNA regions; DGTP

Indexed keywords

BETAINE; DNA; DNA BASE; DYE; PRIMER DNA; REAGENT; REPETITIVE DNA;

EID: 77955595663     PISSN: 15240215     EISSN: 19434731     Source Type: Journal    
DOI: None     Document Type: Article
Times cited : (14)

References (11)
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    • Needleman D, Adam D, Detwiler M, et al. DNA Sequencing Research Group: 2006 General Survey of DNA Sequencing Facilities. J Biomol Tech 2007;18:113-119.
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    • Needleman, D.1    Adam, D.2    Detwiler, M.3
  • 3
    • 68249138823 scopus 로고    scopus 로고
    • Identification of optimal protocols for sequencing difficult templates: The results of the ABRF DSRG Difficult Template Study 2008
    • Kieleczawa J, Adam D, Bintzler D, et al. Identification of optimal protocols for sequencing difficult templates: the results of the ABRF DSRG Difficult Template Study 2008. J Biomol Tech 2009;20:116-127.
    • (2009) J Biomol Tech , vol.20 , pp. 116-127
    • Kieleczawa, J.1    Adam, D.2    Bintzler, D.3
  • 4
    • 77955650662 scopus 로고    scopus 로고
    • Sequencing of difficult DNA templates
    • Kieleczawa J (ed): Sudbury, MA, USA: Jones and Bartlett
    • Kieleczawa J. Sequencing of difficult DNA templates. In Kieleczawa J (ed): DNA Sequencing: Optimizing the Process and Analysis, Sudbury, MA, USA: Jones and Bartlett, 2005;27-34.
    • (2005) DNA Sequencing: Optimizing the Process and Analysis , pp. 27-34
    • Kieleczawa, J.1
  • 5
    • 33644866775 scopus 로고    scopus 로고
    • Simple modifications of the standard DNA sequencing protocol allow for sequencing through siRNA hairpins and other repeats
    • Kieleczawa J. Simple modifications of the standard DNA sequencing protocol allow for sequencing through siRNA hairpins and other repeats. J Biomol Tech 2005;16:220-223.
    • (2005) J Biomol Tech , vol.16 , pp. 220-223
    • Kieleczawa, J.1
  • 6
    • 77955595931 scopus 로고    scopus 로고
    • Controlled heat-denaturation of DNA plasmids
    • Kieleczawa J (ed): Sudbury, MA, USA: Jones and Bartlett
    • Kieleczawa J. Controlled heat-denaturation of DNA plasmids. In Kieleczawa J (ed): DNA Sequencing: Optimizing the Process and Analysis. Sudbury, MA, USA: Jones and Bartlett, 2005;1-10.
    • (2005) DNA Sequencing: Optimizing the Process and Analysis , pp. 1-10
    • Kieleczawa, J.1
  • 7
    • 0027319489 scopus 로고
    • A simplified and reliable protocol for plasmid DNA sequencing: Fast miniprep and denaturation
    • Yie Y, Wei Z, Tien P. A simplified and reliable protocol for plasmid DNA sequencing: Fast miniprep and denaturation. Nucleic Acids Res 1993;21:361.
    • (1993) Nucleic Acids Res , vol.21 , pp. 361
    • Yie, Y.1    Wei, Z.2    Tien, P.3
  • 8
    • 77955630248 scopus 로고    scopus 로고
    • Prolonged storage of plasmid DNAs under different conditions: Effects on plasmid integrity, spectra characteristics, and DNA sequence quality
    • Kieleczawa J (ed): Sudbury, MA, USA: Jones and Bartlett
    • Kieleczawa J, Wu P. Prolonged storage of plasmid DNAs under different conditions: effects on plasmid integrity, spectra characteristics, and DNA sequence quality. In Kieleczawa J (ed): DNA Sequencing II: Optimizing the Preparation and Clean Up, Sudbury, MA, USA: Jones and Bartlett, 2005;99-115.
    • (2005) DNA Sequencing II: Optimizing the Preparation and Clean Up , pp. 99-115
    • Kieleczawa, J.1    Wu, P.2
  • 9
    • 77955647220 scopus 로고    scopus 로고
    • Preparation of difficult DNA templates using seven different commercial methods
    • Kieleczawa J (ed): Sudbury, MA, USA: Jones and Bartlett
    • Kieleczawa J, Li T, Wu, P. Preparation of difficult DNA templates using seven different commercial methods. In Kieleczawa J (ed): DNA Sequencing II: Optimizing the Preparation and Clean Up, Sudbury, MA, USA: Jones and Bartlett, 2006;1-14.
    • (2006) DNA Sequencing II: Optimizing the Preparation and Clean Up , pp. 1-14
    • Kieleczawa, J.1    Li, T.2    Wu, P.3
  • 10
    • 0031955518 scopus 로고    scopus 로고
    • Base-calling of automated sequencer traces using phred. I. Accuracy assessment
    • Ewing B, Hillier L, Wendl MC, Green P. Base-calling of automated sequencer traces using phred. I. Accuracy assessment. Genome Res 1998;8:175-185. (Pubitemid 28177229)
    • (1998) Genome Research , vol.8 , Issue.3 , pp. 175-185
    • Ewing, B.1    Hillier, L.2    Wendl, M.C.3    Green, P.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.