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Most ripening processes could be followed real-time under exposure of the electron beam in the TEM, whereby the configurations formed in the examined areas were the same as configurations present elsewhere on the microheater chip (because the electron beam is small, and the membranes far apart, “fresh� areas could be checked frequently). Only in the case of the separated Au dots and CdSe rods (Fig. 1 g,h), the electron beam irradiation apparently hampered the formation (nucleation) of new Au tips. So although this experiment is well reproducible both in the TEM and the SEM, no before/after images of the same area could be obtained. Possible explanations could be the irradiation-induced carburization of ligands that hampers changes (this seems unlikely because no such problems were encountered with the other nanostructures) or possibly the electron beam generates charging effects (electron-hole pairs in the CdSe rods or charge pile-up at the Au dots) which hampers the formation of new Au tips at the polar-CdSe/Au interface.
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A control experiment, in which a substrate with only Au spherical nanocrystals was heated, is shown in Figure S1 of the Supporting Information.
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