Distance between RBS and AUG plays an important role in overexpression of recombinant proteins

Analytical Biochemistry

Volumn 405, Issue 2, 2010, Pages 275-277

  Berwal, Sunil K ^a   Sreejith, R K ^a   Pal, Jayanta K ^a  

^a UNIVERSITY OF PUNE   (India)

Author keywords

AUG; Overexpression; Prokaryotic expression vectors; Ribosome binding site; Shine Dalgarno sequence; Spacer

Indexed keywords

BIOSYNTHESIS; CLONING; ELECTROPHORESIS; ESCHERICHIA COLI; GENES; MACROMOLECULES;

OVER-EXPRESSION; PROKARYOTIC EXPRESSION; RIBOSOME BINDING SITES; SHINE-DALGARNO SEQUENCE; SPACER;

RECOMBINANT PROTEINS;

NUCLEOTIDE; RECOMBINANT PROTEIN;

ARTICLE; BINDING SITE; CONTROLLED STUDY; GENE CONSTRUCT; GENE OVEREXPRESSION; GENE VECTOR; MOLECULAR CLONING; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROKARYOTE; PROTEIN EXPRESSION; RIBOSOME BINDING SITE; START CODON; SYNTHESIS; WESTERN BLOTTING;

ESCHERICHIA COLI; PROKARYOTA;

EID: 77955588048     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2010.06.003     Document Type: Article

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