Efficient cell-seeding into scaffolds improves bone formation

Journal of Dental Research

Volumn 89, Issue 8, 2010, Pages 854-859

  Hasegawa, T ^a   Miwa, M ^a   Sakai, Y ^a   Niikura, T ^a   Lee, S Y ^a   Oe, K ^a   Iwakura, T ^a   Kurosaka, M ^a   Komori, T ^a  

^a KOBE UNIVERSITY GRADUATE SCHOOL OF MEDICINE   (Japan)

Author keywords

beta TCP; bone marrow stromal cells; bone tissue engineering; cell seeding; osteogenesis

Indexed keywords

ALKALINE PHOSPHATASE; BETA-TRICALCIUM PHOSPHATE; CALCIUM PHOSPHATE; OSTEOCALCIN; TISSUE SCAFFOLD;

ANIMAL; ARTICLE; BIOSYNTHESIS; BONE MARROW CELL; BONE REGENERATION; CELL DIFFERENTIATION; COMPARATIVE STUDY; CULTURE TECHNIQUE; CYTOLOGY; IMMERSION; INSTRUMENTATION; MESENCHYMAL STEM CELL TRANSPLANTATION; METHODOLOGY; PRESSURE; RAT; SPRAGUE DAWLEY RAT; STROMA CELL; SUBCUTANEOUS TISSUE; SYRINGE; TISSUE ENGINEERING; TRANSPLANTATION; VACUUM;

ALKALINE PHOSPHATASE; ANIMALS; BONE MARROW CELLS; BONE REGENERATION; CALCIUM PHOSPHATES; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; IMMERSION; MESENCHYMAL STEM CELL TRANSPLANTATION; OSTEOCALCIN; PRESSURE; RATS; RATS, SPRAGUE-DAWLEY; STROMAL CELLS; SUBCUTANEOUS TISSUE; SYRINGES; TISSUE ENGINEERING; TISSUE SCAFFOLDS; VACUUM;

EID: 77955484211     PISSN: 00220345     EISSN: 15440591     Source Type: Journal    
DOI: 10.1177/0022034510370022     Document Type: Article

References (20)

1. Arrington ED, Smith WJ, Chambers HG, Chambers HG, Bucknell AL, Davino NA (1996). Complications of iliac crest bone graft harvesting. Clin Orthop Relat Res 329: 300-309.
2. Dong J., Uemura T., Kojima H., Kikuchi M., Tanaka J., Tateishi T. (2001). Application of low-pressure system to sustain in vivo bone formation in osteoblast/porous hydroxyapatite composite. Mater Sci Eng 17 (C). 37-43.
3. Dong J., Uemura T., Kikuchi M., Tanaka J., Tateishi T. (2002). Long-term durability of porous hydroxyapatite with low-pressure system to support osteogenesis of mesenchymal stem cells. Biomed Mater Eng 12: 203-209.
4. Heymann D., Delécrin J., Deschamps C., Gouin F., Padrines M., Passuti N. (2001). In vitro assessment of combining osteogenic cells with macroporous calcium-phosphate ceramics. Rev Clin Orthop Reparatrice Appar Mot 87: 8-17.
5. Iida J., Yoshikawa T., Akahane M., Ohgushi H., Dohi Y., Takakura Y., et al. (2004). Osteogenic potential of cultured bone/ceramic construct: comparison with marrow mesenchymal cell/ceramic composite. Cell Transplant 13: 357-365.
6. Kasten P., Luginbühl R., van Griensven M., Barkhausen T., Krettek C., Bohner M., et al. (2003). Comparison of human bone marrow stromal cells seeded on calcium-deficient hydroxyapatite, beta-tricalcium phosphate and demineralized bone matrix. Biomaterials 24: 2593-2603.
7. Kitamura S., Ohgushi H., Hirose M., Funaoka H., Takakura Y., Ito H. (2004). Osteogenic differentiation of human bone marrow-derived mesenchymal cells cultured on alumina ceramics. Artif Organs 28: 72-82.
8. Kon E., Muraglia A., Corsi A., Bianco P., Marcacci M., Martin I., et al. (2000). Autologous bone marrow stromal cells loaded onto porous hydroxyapatite ceramic accelerate bone repair in critical-size defect of sheep long bones. J Biomed Mater Res 49: 328-337.
9. Lee SY, Miwa M., Sakai Y., Kuroda R., Niikura T., Kurosaka M. (2006). Osteogenic potential of cells in vitro derived from haemarthrosis of the knee induced by injury to the anterior cruciate ligament. J Bone Joint Surg Br 88: 129-133.
10. Lode A., Bernhardt A., Gelinsky M. (2008). Cultivation of human bone marrow stromal cells on three-dimensional scaffolds of mineralized collagen: influence of seeding density on colonization, proliferation and osteogenic differentiation. J Tissue Eng Regen Med 2: 400-407.
11. Mankani MH, Kuznetsov SA, Fowler B., Kingman A., Robey PG (2001). In vivo bone formation by human bone marrow stromal cells: effect of carrier particle size and shape. Biotechnol Bioeng 72: 96-107.
12. Niikura T., Miwa M., Sakai Y., Lee SY, Kuroda R., Fujishiro T., et al. (2005). Human hemarthrosis-derived progenitor cells can differentiate into osteoblast-like cells in vitro. Biochem Biophys Res Commun 336: 1234-1240.
13. Ohgushi H., Caplan AI (1999). Stem cell technology and bioceramics: from cell to gene engineering. J Biomed Mater Res 48: 913-927.
14. Toquet J., Rohanizadeh R., Guicheux J., Couillaud S., Passuti N., Daculsi G., et al. (1999). Osteogenic potential in vitro of human bone marrow cells cultured on macroporous biphasic calcium phosphate ceramic. J Biomed Mater Res 44: 98-108.
15. Torigoe I., Sotome S., Tsuchiya A., Yoshii T., Takahashi M., Kawabata S., et al. (2007). Novel cell seeding system into a porous scaffold using a modified low-pressure method to enhance cell seeding efficiency and bone formation. Cell Transplant 16: 729-739.
16. Wang Y., Uemura T., Dong J., Kojima H., Tanaka J., Tateishi T. (2003). Application of perfusion culture system improves in vitro and in vivo osteogenesis of bone marrow-derived osteoblastic cells in porous ceramic materials. Tissue Eng 9: 1205-1214.
17. Yoshii T., Sotome S., Torigoe I., Tsuchiya A., Maehara H., Ichinose S., et al. (2009). Fresh bone marrow introduction into porous scaffolds using a simple low-pressure loading method for effective osteogenesis in a rabbit model. J Orthop Res 27: 1-7.
18. Yoshikawa T., Ohgushi H., Uemura T., Nakajima H., Ichijima K., Tamai S., et al. (1998). Human marrow cells-derived cultured bone in porous ceramics. Biomed Mater Eng 8: 311-320.
19. Younger EM, Chapman MW (1989). Morbidity at bone graft donor site. J Orthop Trauma 3: 192-195.
20. Zhou YF, Sae-Lim V., Chou AM, Hutmacher DW, Lim TM (2006). Does seeding density affect in vitro mineral nodules formation in novel composite scaffolds? J Biomed Mater Res A 78: 183-193.