Investigation of bacterial chemotaxis in flow-based microfluidic devices

Nature Protocols

Volumn 5, Issue 5, 2010, Pages 864-872

  Englert, Derek L ^a   Manson, Michael D ^b   Jayaraman, Arul ^a,c  

^a Texas AandM University   (United States)

^b TEXAS A AND M UNIVERSITY   (United States)

^c Texas A and M University   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHEMOTACTIC FACTOR;

ARTICLE; CHEMOTAXIS; ESCHERICHIA COLI; MICROFLUIDIC ANALYSIS; PHYSIOLOGY;

CHEMOTACTIC FACTORS; CHEMOTAXIS; ESCHERICHIA COLI; MICROFLUIDIC ANALYTICAL TECHNIQUES;

EID: 77955474884     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2010.18     Document Type: Article

References (17)

1. Hazelbauer, G.L., Mesibov, R.E., Adler, J. Escherichia coli mutants defective in chemotaxis towards specific chemicals. Proc. Natl. Acad. Sci. USA 64, 1300-1307 (1969).
2. Wadhams, G.H., Armitage, J.P. Making sense of it all: bacterial chemotaxis. Nat. Rev. Mol. Cell. Biol. 5, 1024-1037 (2004).
3. Brown, D.A., Berg, H.C. Temporal stimulation of chemotaxis in Escherichia coli. Proc. Natl. Acad. Sci. USA 71, 1388-1392 (1974).
4. Macnab, R.M., Koshland, D.E. The gradient-sensing mechanism in bacterial chemotaxis. Proc. Natl. Acad. Sci. USA 69, 2509-2512 (1972).
5. Adler, J., A method for measuring chemotaxis and use of the method to determine optimum conditions for chemotaxis by Escherichia coli. J. Gen. Microbiol. 74, 77-91 (1973).
6. Tso, W.-W., Adler, J. Negative chemotaxis in Escherichia coli. J. Bacteriol. 118, 560-576 (1974).
7. Cheng, S.Y. et al. A hydrogel-based microfluidic device for the studies of directed cell migration. Lab Chip 7, 763-769 (2007).
8. Diao, J. et al. A three-channel microfluidic device for generating static linear gradients and its application to the quantitative analysis of bacterial chemotaxis. Lab Chip 6, 381-388 (2006).
9. Mao, H., Cremer, P.S., Manson, M.D. A sensitive, versatile microfluidic assay for bacterial chemotaxis. Proc. Natl. Acad. Sci. USA 100, 5449-5454 (2003).
10. Englert, D.L., Manson, M.D., Jayaraman, A. A flow-based microfluidic device for quantifying bacterial chemotaxis in stable, competing gradients. Appl. Environ. Microbiol. 75, 4557-4564 (2009).
11. Englert, D., Jayaraman, A., Manson, M.D. (eds.) Microfluidic techniques for the analysis of bacterial chemotaxis. Methods Mol. Biol. 571, 1-23 (2009).
12. Jeon, N.L. et al. Neutrophil chemotaxis in linear and complex gradients of interleukin-8 formed in a microfabricated device. Nat. Biotechnol. 20, 826-830 (2002).
13. Thompson, D.M. et al. Dynamic gene expression profiling using a microfabricated living cell array. Anal. Chem. 76, 4098-4103 (2004).
14. Englert, D.L., Adase, C.A., Jayaraman, A., Manson, M.D. Repellent taxis to nickel ion requires neither Ni2+ transport nor the periplasmic NikA binding protein. J. Bacteriol. (in the press).
15. Hansen, M.C., Palmer, R.J. Jr., Udsen, C., White, D.C., Molin, S. Assessment of GFP fluorescence in cells of Streptococcus gordonii under conditions of low pH and low oxygen concentration Microbiology 147, 1383-1391 (2001).
16. Whitesides, G.M., Ostuni, E., Takayama, S., Jiang, X., Ingber, D.E. Soft lithography in biology and biochemistry. Annu. Rev. Biomed. Eng. 3, 335-373 (2001).
17. Walker, G.M. et al. Effects of flow and diffusion on chemotaxis studies in a microfabricated gradient generator. Lab Chip 5, 611-618 (2005).