Phylogenetic analysis and Shiga toxin production profiling of Shiga toxin-producing/enterohemorrhagic Escherichia coli clinical isolates

Microbial Pathogenesis

Volumn 49, Issue 5, 2010, Pages 246-251

  Li, Rui ^a,b   Harada, Takaaki ^a   Honjoh, Ken Ichi ^a   Miyamoto, Takahisa ^a  

^a KYUSHU UNIVERSITY   (Japan)

^b WUHAN POLYTECHNIC UNIVERSITY   (China)

Author keywords

EHEC; Gene expression; Phylogenesis; Shiga toxin; STEC

Indexed keywords

SHIGA TOXIN; VEROTOXIN 1; VEROTOXIN 2;

ANIMAL CELL; ARTICLE; BACTERIUM ISOLATE; BACTERIUM ISOLATION; CONTROLLED STUDY; CYTOTOXICITY TEST; ENTEROHEMORRHAGIC ESCHERICHIA COLI; GENE EXPRESSION; GENETIC TRANSCRIPTION; GENOTYPE; JAPAN; LATEX AGGLUTINATION TEST; NONHUMAN; NUCLEOTIDE SEQUENCE; PHENOTYPE; PHYLOGENY; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SHIGA TOXIN PRODUCING ESCHERICHIA COLI; STRAIN DIFFERENCE; TOXIN SYNTHESIS; VERO CELL;

ANIMALS; CERCOPITHECUS AETHIOPS; DNA, BACTERIAL; ENTEROHEMORRHAGIC ESCHERICHIA COLI; ESCHERICHIA COLI INFECTIONS; GENE EXPRESSION PROFILING; GENOTYPE; HUMANS; JAPAN; LATEX FIXATION TESTS; MOLECULAR SEQUENCE DATA; PHYLOGENY; POLYMORPHISM, GENETIC; SEQUENCE ANALYSIS, DNA; SHIGA TOXIN; VERO CELLS; VIRULENCE;

ESCHERICHIA COLI;

EID: 77955424661     PISSN: 08824010     EISSN: 10961208     Source Type: Journal    
DOI: 10.1016/j.micpath.2010.06.005     Document Type: Article

References (27)

1. Girardeau J.P., Dalmasso A., Bertin Y., Ducrot C., Bord S., Livrelli V., et al. Association of virulence genotype with phylogenetic background in comparison to different seropathotypes of Shiga toxin-producing Escherichia coli isolates. J Clin Microbiol 2005, 43:6098-6107.
2. Mainil J.G., Daube G. Verotoxigenic Escherichia coli from animals, humans and foods: who's who?. J Appl Microbiol 2005, 98:1332-1344.
3. Eklund M., Scheutz F., Siitonen A. Clinical isolates of non-O157 Shiga toxin-producing Escherichia coli: serotypes, virulence characteristics, and molecular profiles of strains of the same serotype. J Clin Microbiol 2001, 39:2829-2834.
4. Kozub-Witkowski E., Krause G., Frankel G., Kramer D., Appel B., Beutin L. Serotypes and virutypes of enteropathogenic and enterohaemorrhagic Escherichia coli strains from stool samples of children with diarrhoea in Germany. J Appl Microbiol 2008, 104:403-410.
5. Law D. Virulence factors of Escherichia coli O157 and other Shiga toxin-producing E. coli. J Appl Microbiol 2000, 88:729-745.
6. Paton J.C., Paton A.W. Pathogenesis and diagnosis of Shiga toxin-producing Escherichia coli infections. Clin Microbiol Rev 1998, 11:450-479.
7. Ishii S., Meyer K.P., Sadowsky M.J. Relationship between phylogenetic groups, genotypic clusters, and virulence gene profiles of Escherichia coli strains from diverse human and animal sources. Appl Environ Microbiol 2007, 73:5703-5710.
8. Kawano K., Okada M., Haga T., Maeda K., Goto Y. Relationship between pathogenicity for humans and stx genotype in Shiga toxin-producing Escherichia coli serotype O157. Eur J Clin Microbiol Infect Dis 2008, 27:227-232.
9. Beutin L., Krause G., Zimmermann S., Kaulfuss S., Gleier K. Characterization of Shiga toxin-producing Escherichia coli strains isolated from human patients in Germany over a 3-year period. J Clin Microbiol 2004, 42:1099-1108.
10. Friedrich A.W., Bielaszewska M., Zhang W.L., Pulz M., Kuczius T., Ammon A., et al. Escherichia coli harboring Shiga toxin 2 gene variants: frequency and association with clinical symptoms. J Infect Dis 2002, 185:74-84.
11. Friedrich A.W., Borell J., Bielaszewska M., Fruth A., Tschape H., Karch H. Shiga toxin 1c-producing Escherichia coli strains: phenotypic and genetic characterization and association with human disease. J Clin Microbiol 2003, 41:2448-2453.
12. Friedrich A.W., Nierhoff K.V., Bielaszewska M., Mellmann A., Karch H. Phylogeny, clinical associations, and diagnostic utility of the pilin subunit gene (sfpA) of sorbitol-fermenting, enterohemorrhagic Escherichia coli O157:H. J Clin Microbiol 2004, 42:4697-4701.
13. Eklund M., Leino K., Siitonen A. Clinical Escherichia coli strains carrying stx genes: stx variants and stx-positive virulence profiles. J Clin Microbiol 2002, 40:4585-4593.
14. Beutin L., Miko A., Krause G., Pries K., Haby S., Steege K., et al. Identification of human-pathogenic strains of Shiga toxin-producing Escherichia coli from food by a combination of serotyping and molecular typing of Shiga toxin genes. Appl Environ Microbiol 2007, 73:4769-4775.
15. Herold S., Karch H., Schmidt H. Shiga toxin-encoding bacteriophages-genomes in motion. Int J Med Microbiol 2004, 294:115-121.
16. Karmali M.A., Petric M., Bielaszewska M. Evaluation of a microplate latex agglutination method (Verotox-F assay) for detecting and characterizing verotoxins (Shiga toxins) in Escherichia coli. J Clin Microbiol 1999, 37:396-399.
17. Hahm B.K., Maldonado Y., Schreiber E., Bhunia A.K., Nakatsu C.H. Subtyping of foodborne and environmental isolates of Escherichia coli by multiplex-PCR, rep-PCR, PFGE, ribotyping and AFLP. J Microbiol Methods 2003, 53:387-399.
18. Persson S., Olsen K.E., Ethelberg S., Scheutz F. Subtyping method for Escherichia coli shiga toxin (verocytotoxin) 2 variants and correlations to clinical manifestations. J Clin Microbiol 2007, 45:2020-2024.
19. Koitabashi T., Cui S., Kamruzzaman M., Nishibuchi M. Isolation and characterization of the Shiga toxin gene (stx)-bearing Escherichia coli O157 and non-O157 from retail meats in Shandong Province, China, and characterization of the O157-derived stx2 phages. J Food Prot 2008, 71:706-713.
20. Koitabashi T., Vuddhakul V., Radu S., Morigaki T., Asai N., Nakaguchi Y., et al. Genetic characterization of Escherichia coli O157: H7/- strains carrying the stx2 gene but not producing Shiga toxin 2. Microbiol Immunol 2006, 50:135-148.
21. Muniesa M., Jofre J. Abundance in sewage of bacteriophages infecting Escherichia coli O157:H7. Methods Mol Biol 2004, 268:79-88.
22. Nakao H., Kimura K., Murakami H., Maruyama T., Takeda T. Subtyping of Shiga toxin 2 variants in human-derived Shiga toxin-producing Escherichia coli strains isolated in Japan. FEMS Immunol Med Microbiol 2002, 34:289-297.
23. Schmidt H. Shiga-toxin-converting bacteriophages. Res Microbiol 2001, 152:687-695.
24. Wagner P.L., Neely M.N., Zhang X., Acheson D.W., Waldor M.K., Friedman D.I. Role for a phage promoter in Shiga toxin 2 expression from a pathogenic Escherichia coli strain. J Bacteriol 2001, 183:2081-2085.
25. Lejeune J.T., Abedon S.T., Takemura K., Christie N.P., Sreevatsan S. Human Escherichia coli O157:H7 genetic marker in isolates of bovine origin. Emerg Infect Dis 2004, 10:1482-1485.
26. Ahmad A., Zurek L. Evaluation of the anti-terminator Q933 gene as a marker for Escherichia coli O157:H7 with high Shiga toxin production. Curr Microbiol 2006, 53:324-328.
27. Matsumoto M., Suzuki M., Takahashi M., Hirose K., Minagawa H., Ohta M. Identification and epidemiological description of enterohemorrhagic Escherichia coli O157 strains producing low amounts of Shiga toxin 2 in Aichi Prefecture, Japan. Jpn J Infect Dis 2008, 61:442-445.