Heterologous expression and characterization of recombinant OsCDR1, a rice aspartic proteinase involved in disease resistance

Protein Expression and Purification

Volumn 72, Issue 2, 2010, Pages 169-174

  Prasad, Bishun Deo ^a   Creissen, Gary ^b   Lamb, Chris ^b   Chattoo, Bharat B ^a  

^a M S UNIVERSITY OF BARODA   (India)

^b JOHN INNES CENTRE   (United Kingdom)

Author keywords

Aspartic proteinase (AP); Glutathione S transferase (GST); Oryza sativa constitutive disease resistance 1 (OsCDR1); Pathogenesis related (PR) gene; Salicylic acid (SA)

Indexed keywords

ANTIFUNGAL AGENT; ASPARTIC PROTEINASE; GLUTATHIONE TRANSFERASE; HYBRID PROTEIN; VEGETABLE PROTEIN;

AMINO ACID SEQUENCE; ARABIDOPSIS; ARTICLE; BIOSYNTHESIS; CHEMISTRY; DRUG EFFECT; ENZYMOLOGY; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; GENETICS; INNATE IMMUNITY; METABOLISM; MOLECULAR GENETICS; RICE; SITE DIRECTED MUTAGENESIS; TEMPERATURE;

AMINO ACID SEQUENCE; ANTIFUNGAL AGENTS; ARABIDOPSIS; ASPARTIC ACID PROTEASES; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, PLANT; GLUTATHIONE TRANSFERASE; IMMUNITY, INNATE; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; ORYZA SATIVA; PLANT PROTEINS; RECOMBINANT FUSION PROTEINS; TEMPERATURE;

ARABIDOPSIS; ESCHERICHIA COLI; ORYZA SATIVA;

EID: 77955422096     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2010.03.018     Document Type: Article

References (20)

1. D.R. Davies The structure and function of the aspartic proteinases Annu. Rev. 19 1990 189 215
2. A.J. Barrett Cellular proteolysis: an overview Ann. NY Acad. Sci. 674 1992 1 15
3. N.D. Rawlings, and A.J. Barrett MEROPS: the peptidase database Nucleic Acids Res. 27 1999 325 331
4. K. John Aspartic proteinases and their inhibitors V. Kostka, FEBS Advanced Course 84/07 1985 Walter de Gruyter Berlin 1 17
5. B. Foltman Comments on the nomenclature of aspartic proteinases V. Kostka, Aspartic Proteinases and Their Inhibitors, FEBS Advanced Course 84/07 1985 Walter de Gruyter Berlin 19 26
6. C. Faro, and S. Gal Aspartic proteinase content of the Arabidopsis genome Curr. Protein Pept. Sci. 6 2005 493 500
7. A. Mutlu, and S. Gal Plant aspartic proteinases: enzymes on the way to a function Physiol. Plant 105 1999 569 576
8. F. Chen, and M.R. Foolad Molecular organization of a gene in barley which encodes a protein similar to aspartic protease and its specific expression in nucellar cells during degeneration Plant Mol. Biol. 35 1997 821 831
9. I. Simões, and C. Faro Structure and function of plant aspartic proteinases Eur. J. Biochem. 271 2004 2067 2075
10. B.D. Prasad, G. Creissen, C. Lamb, and B.B. Chattoo Overexpression of rice (Oryza sativa L.) OsCDR1 leads to constitutive activation of defence responses in rice and Arabidopsis Mol. Plant Microbe Interact. 22 2009 1635 1644
11. W.E. Durrant, and X. Dong Systemic acquired resistance Annu. Rev. Phytopathol. 42 2004 185 209
12. A.M. Maldonado, P. Doerner, R.A. Dixon, C.J. Lamb, and R.K. Cameron A putative lipid transfer protein involved in systemic resistance signalling in Arabidopsis Nature 419 2002 399 403
13. K.J. Livak, and T.D. Schmittgen Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCt method Methods 25 2001 402 408
14. L.F. Johnson, and E.A. Curl Methods for Research on the Ecology of Soil-Borne Plant Pathogens 1972 Burgess Publishing Co. Minneapolis, MN pp. 178
15. I. Simões, R. Faro, D. Bur, and C. Faro Characterization of recombinant CDR1, an Arabidopsis aspartic proteinase involved in disease resistance J. Biol. Chem. 282 2007 31358 31365
16. M.G. Guevara, C.R. Oliva, M. Huarte, and G.R. Daleo An aspartic protease with antimicrobial activity is induced after infection and wounding in intercellular fluids of potato tubers Eur. J. Plant Pathol. 108 2002 131 137
17. M.G. Guevara, P. Veríssimo, E. Pires, C. Faro, and G.R. Daleo Potato aspartic proteases: induction, antimicrobial activity and substrate specificity J. Plant Pathol. 86 2004 233 238
18. J.R. Mendieta, M.R. Pagano, F.F. Muñoz, G.R. Daleo, and M.G. Guevara Antimicrobial activity of potato aspartic proteases (StAPs) involves membrane permeabilization Microbiology 152 2006 2039 2047
19. Y. Xia, H. Suzuki, J. Borevitz, J. Blount, Z. Guo, K. Patel, R.A. Dixon, and C. Lamb An extracellular aspartic protease functions in Arabidopsis disease resistance signalling EMBO J. 23 2004 980 988
20. Ozaki Digestive Physiology of Fish 1983 Shanghai Science and Technology Press Shanghai, China