Assay of dense-core vesicle exocytosis using permeabilized PC12 cells

Advances in Enzyme Regulation

Volumn 50, Issue 1, 2010, Pages 237-246

  Gao, Jing ^a   Takeuchi, Hiroshi ^a   Umebayashi, Hisanori ^a   Zhang, Zhao ^a   Matsuda, Miho ^a   Hirata, Masato ^a  

^a KYUSHU UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATE; CALCIUM; TISSUE EXTRACT;

ANIMAL; ARTICLE; BIOASSAY; BRAIN; CELL MEMBRANE PERMEABILITY; CELL STRAIN; CHEMISTRY; EXOCYTOSIS; METABOLISM; METHODOLOGY; PHYSIOLOGY; RAT; SECRETORY VESICLE; ULTRASTRUCTURE;

ADENOSINE TRIPHOSPHATE; ANIMALS; BIOLOGICAL ASSAY; BRAIN; CALCIUM; CELL MEMBRANE PERMEABILITY; EXOCYTOSIS; PC12 CELLS; RATS; SECRETORY VESICLES; TISSUE EXTRACTS;

EID: 77955297977     PISSN: 00652571     EISSN: None     Source Type: Book Series    
DOI: 10.1016/j.advenzreg.2009.10.011     Document Type: Article

References (42)

1. Ahnert-Hilger G., Bhakdi S., Gratzl M. Minimal requirements for exocytosis. A study using PC12 cells permeabilized with staphylococcal alpha-toxin. J Biol Chem 1985, 260:12730-12734.
2. Brooks J.C., Treml S. Catecholamine secretion by chemically skinned cultured chromaffin cells. J Neurochem 1983, 40:468-473.
3. 2+-dependent manner. In vitro characteristics and possible significance. J Biol Chem 1998, 273:10240-10248.
4. Dunn L.A., Holz R.W. Catecholamine secretion from digitonin-treated adrenal medullary chromaffin cells. J Biol Chem 1983, 258:4989-4993.
5. Eberhard D.A., Cooper C.L., Low M.G., Holz R.W. Evidence that the inositol phospholipids are necessary for exocytosis. Loss of inositol phospholipids and inhibition of secretion in permeabilized cells caused by a bacterial phospholipase C and removal of ATP. Biochem J 1990, 268:15-25.
6. Fei H., Grygoruk A., Brooks E.S., Chen A., Krantz D.E. Trafficking of vesicular neurotransmitter transporters. Traffic 2008, 9:1425-1436.
7. Fujita Y., Sasaki T., Fukui K., Kotani H., Kimura T., Hata Y., et al. Phosphorylation of Munc-18/n-Sec1/rbSec1 by protein kinase C: its implication in regulating the interaction of Munc-18/n-Sec1/rbSec1 with syntaxin. J Biol Chem 1996, 271:7265-7268.
8. Gao J., Takeuchi H., Zhang Z., Fujii M., Kanematsu T., Hirata M. Binding of phospholipase C-related but catalytically inactive protein to phosphatidylinositol 4,5-bisphosphate via the PH domain. Cell Signal 2009, 21:1180-1186.
9. Gillis K.D., Mossner R., Neher E. Protein kinase C enhances exocytosis from chromaffin cells by increasing the size of the readily releasable pool of secretory granules. Neuron 1996, 16:1209-1220.
10. Greene L.A., Tischler A.S. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc Natl Acad Sci U S A 1976, 73:2424-2428.
11. Greene L.A., Rein G. Synthesis, storage and release of acetylcholine by a noradrenergic pheochromocytoma cell line. Nature 1977, 268:349-351.
12. Grishanin R.N., Kowalchyk J.A., Klenchin V.A., Ann K., Earles C.A., Chapman E.R., et al. CAPS acts at a prefusion step in dense-core vesicle exocytosis as a PIP2 binding protein. Neuron 2004, 43:551-562.
13. Hashimoto T., Hirata M., Ito Y. A role for inositol 1,4,5-trisphosphate in the initiation of agonist-induced contractions of dog tracheal smooth muscle. Br J Pharmacol 1985, 86:191-199.
14. Hay J.C., Martin T.F. Resolution of regulated secretion into sequential MgATP-dependent and calcium-dependent stages mediated by distinct cytosolic proteins. J Cell Biol 1992, 119:139-151.
15. Hay J.C., Martin T.F. Phosphatidylinositol transfer protein required for ATP-dependent priming of Ca(2+)-activated secretion. Nature 1993, 366:572-575.
16. Hay J.C., Fisette P.L., Jenkins G.H., Fukami K., Takenawa T., Anderson R.A., et al. ATP-dependent inositide phosphorylation required for Ca(2+)-activated secretion. Nature 1995, 374:173-177.
17. 2+ accumulation in intracellular membranes of guinea pig macrophages after saponin treatment. Biochem Biophys Res Commun 1982, 104:1544-1549.
18. 2+ release in saponin-treated macrophages by the photoaffinity derivative of inositol-1, 4, 5-trisphosphate. Nature 1985, 317:723-725.
19. 2+ at concentrations buffered in the micromolar range. Biochim Biophys Acta 1987, 927:177-183.
20. 2+ and GTP-gamma-S for exocytosis from permeabilised mast cells. Cell Signal 1989, 1:157-163.
21. Jahn R., Scheller R.H. SNAREs-engines for membrane fusion. Nat Rev Mol Cell Biol 2006, 7:631-643.
22. James D.J., Khodthong C., Kowalchyk J.A., Martin T.F. Phosphoatidylinositol 4,5-bisphosphate regulates SNARE-dependent membarne fusion. J Cell Biol 2008, 182:355-366.
23. Jockusch W.J., Speidel D., Sigler A., Sorensen J.B., Varoqueaux F., Rhee J.-S., et al. CAPS-1 and CAPS-2 are essential synaptic vesicle priming proteins. Cell 2007, 131:796-808.
24. Kanematsu T., Takeya H., Watanabe Y., Ozaki S., Yoshida M., Koga T., et al. Putative inositol 1,4,5-triphosphate binding proteins in rat brain cytosol. J Biol Chem 1992, 267:6518-6525.
25. Klenchin V.A., Kowalchyk J.A., Martin T.F. Large dense-core vesicle exocytosis in PC12 cells. Methods 1998, 16:204-208.
26. Klenchin V.A., Martin T.F. Priming in exocytosis: attaining fusion-competence after vesicle docking. Biochimie 2000, 82:399-407.
27. Knight D.E., Scrutton M.C. Gaining access to the cytosol: the technique and some applications of electropermeabilization. Biochem J 1986, 234:497-506.
28. Loyet K.M., Kowalchyk J.A., Chaudhary A., Chen J., Prestwich G.D., Martin T.F. Specific binding of phosphatidylinositol 4,5-bisphosphate to calcium-dependent activator protein for secretion (CAPS), a potential phosphoinositide effector protein for regulated exocytosis. J Biol Chem 1998, 273:8337-8343.
29. Mandela P., Ordway G.A. The norepinephrine transporter and its regulation. J Neurochem 2006, 97:310-333.
30. 2+-activated secretion in GH3 pituitary cells. J Biol Chem 1989, 264:10299-10308.
31. Matsuda M., Tsutsumi K., Kanematsu T., Fukami K., Terada Y., Takenawa T., et al. Involvement of phospholipase C-related inactive protein in the mouse reproductive system through the regulation of gonadotropin levels. Biol Reprod 2009, 81:681-689.
32. Milosevic I., Sorensen J.B., Lang T., Krauss M., Nagy G., Haucke V., et al. Plasmalemmal phosphatidylinositol-4,5-bisphosphate level regulates the releasable vesicle pool size in chromaffin cells. J Neurosci 2005, 25:2557-2565.
33. Nojiri M., Loyet K.M., Klenchin V.A., Kabachinski G., Martin T.F. CAPS activity in priming vesicle exocytosis requires CK2 phosphorylation. J Biol Chem 2009, 284:18707-18714.
34. Rizo J., Chen X., Arac D. Unraveling the mechanisms of synaptotagmin and SNARE function in neurotransmitter release. Trends Cell Biol 2006, 16:339-350.
35. Sadakata T., Kakegawa W., Mizoguchi A., Washida M., Katih-SEmba R., Shutoh F., et al. Impaired cerebellar development and function in mice lacking CAPS2, a protein involved in neurotrophine release. J Neurosci 2007, 27:2472-2482.
36. Schiavo G., Gu Q.M., Prestwich G.D., Sollner T.H., Rothman J.E. Calcium-dependent switching of the specificity of phosphoinositide binding to synaptotagmin. Proc Natl Acad Sci U S A 1996, 93:13327-13332.
37. Sollner T.H. Regulated exocytosis and SNARE function. Mol Membr Biol 2003, 20:209-220.
38. Südhof T.C., Rothman J.E. Membrane fusion: grappling with SNARE and SM proteins. Science 2009, 323:474-477.
39. 2+ from intracellular store sites in skinned single cells of porcine coronary artery. Biochem Biophys Res Commun 1984, 120:481-485.
40. Sugita S. Mechanisms of exocytosis. Acta Physiol (Oxf) 2008, 192:185-193.
41. Wagner P.D., Vu N.D. Regulation of norepinephrine secretion in permeabilized PC12 cells by Ca2(+)-stimulated phosphorylation. Effects of protein phosphatases and phosphatase inhibitors. J Biol Chem 1990, 265:10352-10357.
42. Walent J.H., Porter B.W., Martin T.F. A novel 145 kd brain cytosolic protein reconstitutes Ca(2+)-regulated secretion in permeable neuroendocrine cells. Cell 1992, 70:765-775.