Monitoring clinical outcomes, pathological changes and shedding of Chlamydophila abortus following experimental challenge of periparturient ewes utilizing the natural route of infection

Veterinary Microbiology

Volumn 147, Issue 1-2, 2011, Pages 119-126

  Gutierrez, Jorge ^a   Williams, Erin J ^a   O'Donovan, James ^b   Brady, Colm ^b   Proctor, Aisling F ^a   Marques, Patricia X ^a   Worrall, Sheila ^a   Nally, Jarlath E ^a   McElroy, M ^b   Bassett, Hugh F ^a   Sammin, Donal J ^b   Markey, Bryan K ^a  

^a UNIVERSITY COLLEGE DUBLIN   (Ireland)

^b CENTRAL VETERINARY RESEARCH LABORATORY   (Ireland)

Author keywords

Breeding; Chlamydophila abortus; Enzootic abortion; Placenta; Real time PCR; Sheep

Indexed keywords

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BACTERIAL SHEDDING; BACTERIUM DETECTION; BIRTH; BREEDING; CHLAMYDOPHILA; CHLAMYDOPHILA ABORTUS; CONJUNCTIVA; CONTROLLED STUDY; ENZOOTIC ABORTION OF EWES; ESTRUS; EWE; FEMALE; FETUS MEMBRANE; GENE DOSAGE; HISTOPATHOLOGY; LAMB; MOUTH; NONHUMAN; PHAGE DISPLAY; PREGNANCY; PREGNANCY OUTCOME; REAL TIME POLYMERASE CHAIN REACTION; VAGINA SMEAR;

ABORTION, VETERINARY; ANIMALS; ANTIBODIES, BACTERIAL; CHLAMYDOPHILA; CHLAMYDOPHILA INFECTIONS; EXTRAEMBRYONIC MEMBRANES; FEMALE; PREGNANCY; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SHEEP; SHEEP DISEASES;

ANIMALIA; BACTERIA (MICROORGANISMS); CHLAMYDOPHILA ABORTUS; OVIS ARIES;

EID: 77955297222     PISSN: 03781135     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.vetmic.2010.06.015     Document Type: Article

References (35)

1. Aitken I.D. Chlamydial abortion. Diseases of Sheep 2000, 81-86. Blackwell Publishing, Oxford. I.D. Aitken (Ed.).
2. Aitken I.D., Longbottom D. Chlamydial abortion. Diseases of Sheep 2007, 105-111. Blackwell Publishing, Oxford. I.D. Aitken (Ed.).
3. Bovarnick M.R., Miller J.C., Snyder J.C. The influence of certain salts, amino acids, sugars, and proteins on the stability of rickettsiae. J. Bacteriol. 1950, 59:509-522.
4. Buxton D., Barlow R.M., Finlayson J., Anderson I.E., Mackellar A. Observations on the pathogenesis of Chlamydia psittaci infection of pregnant sheep. J. Comp. Pathol. 1990, 102(2):221-237.
5. Buxton D., Anderson I.E., Longbottom D., Livingstone M., Wattegedera S., Entrican G. Ovine chlamydial abortion: characterization of the inflammatory immune response in placental tissues. J. Comp. Pathol. 2002, 127:133-141.
6. Clarkson M.J., Philips H.L. Isolation of faecal chlamydia from sheep in Britain and their characterization by cultural properties. Vet. J. 1997, 153(3):249-251.
7. Guseva N.V., Knight S.T., Whittimore J.D., Wyrick P.B. Primary cultures of female swine genital epithelial cells in vitro: a new approach for the study of hormonal modulation of Chlamydia infection. Infect. Immun. 2003, 71(8):4700-4710.
8. Hobson D., Johnson F.W., Byng R.E. The growth of the ewe abortion chlamydial agent in McCoy cell cultures. J. Comp. Pathol. 1977, 87(1):155-159.
9. Hoffmann B., Depner K., Schirrmeier H., Beer M. A universal heterologous internal control system for duplex real-time RT-PCR assays used in a detection system for pestiviruses. J. Virol. Methods 2006, 136:200-209.
10. Jones G.E., Anderson I.E. Chlamydia psittaci: is tonsillar tissue the portal of entry in ovine enzootic abortion?. Res. Vet. Sci. 1988, 44(2):260-261.
11. Kerr K., Entrican G., McKeever D., Longbottom D. Immunopathology of Chlamydophila abortus infection in sheep and mice. Res. Vet. Sci. 2005, 78:1-7.
12. Kramer P., Lubkin V., Potter W., Jacobs M., Labay G., Silverman P. Cyclic changes in conjunctival smears from menstruating females. Ophthalmology 1990, 97(3):303-337.
13. Livingstone M., Wheelhouse N., Maley S.W., Longbottom D. Molecular detection of Chlamydophila abortus in post-abortion sheep at oestrus and subsequent lambing. Vet. Microbiol. 2009, 135:134-141.
14. Longbottom D., Coulter L.J. Animal chlamydioses and zoonotic implications. J. Comp. Pathol. 2003, 128:217-244.
15. Longbottom D., Livingstone M. Vaccination against chlamydial infections of man and animals. Vet. J. 2006, 171:263-275.
16. Maley S.W., Livingstone M., Rodger S.M., Longbottom D., Buxton D. Identification of Chlamydophila abortus and the development of lesions in placental tissues of experimentally infected sheep. Vet. Microbiol. 2009, 135:122-127.
17. Maslow A.S., Davis C.H., Choong J., Wyrick P.B. Estrogen enhances attachment of Chlamydia trachomatis to human endometrial epithelial cells in vitro. Am. J. Obstet. Gynecol. 1988, 159(4):1006-1014.
18. McClenaghan M., Herring A.J., Aitken I.D. Comparison of Chlamydia psittaci isolates by DNA restriction endonuclease analysis. Infect. Immun. 1984, 45(2):384-389.
19. McEwen A.D., Stamp J.T., Littlejohn A.I. Enzootic abortion in ewes II. Immunization and infection experiments. Vet. Rec. 1951, 63(11):197-201.
20. Milne C.E., Gunn G.J., Entrican G., Longbottom D. Epidemiological modeling of chlamydial abortion in sheep flocks. Vet. Microbiol. 2009, 135:128-133.
21. Navarro J.A., Garcia de la Fuente J.N., Sanchez J., Martinez C.M., Buendia A.J., Gutierrez-Martin C.B., Rodriguez-Ferri E.F., Ortega N., Salinas J. Kinetics of infection and effects on the placenta of Chlamydophila abortus in experimentally infected pregnant ewes. Vet. Pathol. 2004, 41:498-505.
22. Nietfeld J.C. Chlamydial infections in small ruminants. Vet. Clin. N. Am. Food Anim. Pract. 2001, 17:301-314.
23. O'Shea J.D., Wright P.J. Involution and regeneration of the endometrium following parturition in the ewe. Cell Tissue Res. 1984, 236(2):477-485.
24. Pantchev A., Sting R., Bauerfeind R., Tyczka J., Sachse K. New real-time PCR tests for species-specific detection of Chlamydophila psittaci and Chlamydophila abortus from tissue samples. Vet. J. 2009, 181(2):145-150.
25. Papp J.R., Shewen P.E. Pregnancy failure following vaginal infection of sheep with Chlamydia psittaci prior to breeding. Infect. Immun. 1996, 64:1116-1125.
26. Papp J.R., Shewen P.E., Gartley C.J. Abortion and subsequent excretion of chlamydiae from the reproductive tract of sheep during oestrus. Infect. Immun. 1994, 62:3786-3792.
27. Polkinghorne A., Borel N., Becker A., Lu Z.H., Zimmermann D.R., Brugnera E., Pospischil A., Vaughan L. Molecular evidence for chlamydial infections in the eyes of sheep. Vet. Microbiol. 2009, 135:142-146.
28. Ripa K.T., Mårdh P.A. Cultivation of Chlamydia trachomatis in cycloheximide-treated McCoy cells. J. Clin. Microbiol. 1977, 6(4):328-331.
29. Rocchi M.S., Wattegedera S., Meridiani I., Entrican G. Protective adaptive immunity to Chlamydophila abortus infection and control of ovine enzootic abortion (OEA). Vet. Microbiol. 2009, 35(1-2):112-121.
30. Sachse K., Hotzel H. Detection and differentiation of Chlamydiae by nested PCR. Methods Mol. Biol. 2003, 216:123-136.
31. Sammin D.J., Markey B.K., Bassett H.F., McElroy M.C. Rechallenge of previously-infected ewes with Chlamydophila abortus. Vet. Res. Commun. 2005, 29:81-98.
32. Sammin D.J., Markey B.K., Quinn P.J., McElroy M.C., Bassett H.F. Comparison of foetal and maternal inflammatory responses in the ovine placenta after experimental infection with Chlamydophila abortus. J. Comp. Pathol. 2006, 135(2-3):83-92.
33. Vavilis D., Maloutas S., Nasioutziki M., Boni E., Bontis J. Conjunctiva is an estrogen-sensitive epithelium. Acta Obstet. Gynecol. Scand. 1995, 74(10):799-802.
34. Wehrl W., Brinkmann V., Jungblut P.R., Meyer T.F., Szczepek A.J. From the inside out-processing of the Chlamydial autotransporter PmpD and its role in bacterial adhesion and activation of human host cells. Mol. Microbiol. 2004, 51(2):319-334.
35. Wilsmore A.J., Parsons V., Dawson M. Experiments to demonstrate routes of transmission of ovine enzootic abortion. Br. Vet. J. 1984, 140:380-391.