Real-time PCR assays for the detection and quantification of Streptococcus pneumoniae

FEMS Microbiology Letters

Volumn 310, Issue 1, 2010, Pages 48-53

  Park, Hee Kuk ^a   Lee, Hee Jung ^a   Kim, Wonyong ^a  

^a Chung Ang University College of Medicine   (South Korea)

Author keywords

cpsA; pneumonia; qPCR; Streptococcus; TaqMan

Indexed keywords

ARTICLE; BACTERIAL CELL; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM DETECTION; CAPSULAR POLYSACCHARIDE BIOSYNTHESIS GENE; CELL COUNT; DNA EXTRACTION; NONHUMAN; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; STREPTOCOCCUS MITIS; STREPTOCOCCUS ORALIS; STREPTOCOCCUS PNEUMONIAE;

BACTERIAL PROTEINS; COLONY COUNT, MICROBIAL; HUMANS; PNEUMOCOCCAL INFECTIONS; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; STREPTOCOCCUS PNEUMONIAE;

STREPTOCOCCUS; STREPTOCOCCUS MITIS; STREPTOCOCCUS ORALIS; STREPTOCOCCUS PNEUMONIAE; STREPTOCOCCUS PSEUDOPNEUMONIAE;

EID: 77955242490     PISSN: 03781097     EISSN: 15746968     Source Type: Journal    
DOI: 10.1111/j.1574-6968.2010.02044.x     Document Type: Article

References (28)

1. Abdeldaim GM, Stralin K, Olcen P, Blomberg J Herrmann B (2008) Toward a quantitative DNA-based definition of pneumococcal pneumonia: a comparison of Streptococcus pneumoniae target genes, with special reference to the Spn9802 fragment. Diagn Micr Infec Dis 60 : 143 150.
2. Arbique JC, Poyart C, Trieu-Cuot P, Quesne G, Carvalho Mda G, Steigerwalt AG, Morey RE, Jackson D, Davidson RJ Facklam RR (2004) Accuracy of phenotypic and genotypic testing for identification of Streptococcus pneumoniae and description of Streptococcus pseudopneumoniae sp. nov. J Clin Microbiol 42 : 4686 4696.
3. Austrian R (1981) Some observations on the Pneumococcus and on the current status of pneumococcal disease and its prevention. Rev Infect Dis 3 (suppl) : S1 S17.
4. Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG Struhl K (1993) Miniprep of Bacterial Genomic DNA. Current Protocols in Molecular Biology, Vol. 1 (Wilson K, ed.), pp. 2.4.1 2.4.2. John Wiley & Sons, New York.
5. Carvalho Mda G, Tondella ML, McCaustland K et al 2007) Evaluation and improvement of real-time PCR assays targeting lytA, ply, and psaA genes for detection of pneumococcal DNA. J Clin Microbiol 45 : 2460 2466.
6. Corless CE, Guiver M, Borrow R, Edwards-Jones V, Fox AJ Kaczmarski EB (2001) Simultaneous detection of Neisseria meningitidis, Haemophilus influenzae, and Streptococcus pneumoniae in suspected cases of Meningitis and Septicemia using real-time PCR. J Clin Microbiol 39 : 1553 1558.
7. Garcia E Lopez R (1997) Molecular biology of the capsular genes of Streptococcus pneumoniae. FEMS Microbiol Lett 149 : 1 10.
8. Greiner O, Day PJ, Bosshard PP, Imeri F, Altwegg M Nadal D (2001) Quantitative detection of Streptococcus pneumoniae in Nasopharyngeal secretions by real-time PCR. J Clin Microbiol 39 : 3129 3134.
9. Henrichsen J (1995) Six newly recognized types of Streptococcus pneumoniae. J Clin Microbiol 33 : 2759 2762.
10. Kawamura Y, Whiley RA, Shu SE, Ezaki T Hardie JM (1999) Genetic approaches to the identification of the Mitis group within the genus Streptococcus. Microbiology 145 : 2605 2613.
11. Lambertz ST, Nilsson C, Hallanvuo S Lindblad M (2008) Real-time PCR method for detection of pathogenic Yersinia enterocolitica in food. Appl Environ Micro 74 : 6060 6067.
12. Mager DL, Ximenez-Fyvie LA, Haffajee AD Socransky SS (2003) Distribution of selected bacterial species on intraoral surfaces. J Clin Periodontol 30 : 644 654.
13. McAvin JC, Reilly PA, Roudabush RM et al 2001) Sensitive and specific method for rapid identification of Streptococcus pneumoniae using real-time fluorescence PCR. J Clin Microbiol 39 : 3446 3451.
14. Morens DM, Taubenberger JK Fauci AS (2008) Predominant role of bacterial pneumonia as a cause of death in pandemic influenza: implications for pandemic influenza preparedness. J Infect Dis 198 : 962 970.
15. Morona JK, Morona R Paton JC (1997) Characterization of the locus encoding the Streptococcus pneumoniae type 19f capsular polysaccharide biosynthetic pathway. Mol Microbiol 23 : 751 763. (Pubitemid 27072936)
16. Morrison KE, Lake D, Crook J, Carlone GM, Ades E, Facklam R Sampson JS (2000) Confirmation of psaA in All 90 serotypes of Streptococcus pneumoniae by PCR and potential of this assay for identification and diagnosis. J Clin Microbiol 38 : 434 437.
17. Munoz R, Mollerach M, Lopez R Garcia E (1997) Molecular organization of the genes required for the synthesis of type 1 capsular polysaccharide of Streptococcus pneumoniae: formation of binary encapsulated pneumococci and identification of cryptic dTDP-rhamnose biosynthesis genes. Mol Microbiol 25 : 79 92.
18. O'Neill AM, Gillespie SH Whiting GC (1999) Detection of penicillin susceptibility in Streptococcus pneumoniae by pbp2b PCR-restriction fragment length polymorphism analysis. J Clin Microbiol 37 : 157 160.
19. Palacios G, Hornig M, Cisterna D et al 2009) Streptococcus pneumoniae coinfection is correlated with the severity of H1N1 pandemic influenza. PLoS One 4 : e8540.
20. Rodriguez-Lazaro D, Lewis DA, Ocampo-Sosa AA, Fogarty U, Makrai L, Navas J, Scortti M, Hernandez M Vazquez-Boland JA (2006) Internally controlled real-time PCR method for quantitative species-specific detection and vapA genotyping of Rhodococcus equi. Appl Environ Micro 72 : 4256 4263.
21. Sails AD, Fox AJ, Bolton FJ, Wareing DR Greenway DL (2003) A real-time PCR assay for the detection of Campylobacter jejuni in foods after enrichment culture. Appl Environ Microb 69 : 1383 1390.
22. Seki M, Yamashita Y, Torigoe H, Tsuda H, Sato S Maeno M (2005) Loop-mediated isothermal amplification method targeting the lytA gene for detection of Streptococcus pneumoniae. J Clin Microbiol 43 : 1581 1586.
23. Suzuki N, Seki M, Nakano Y, Kiyoura Y, Maeno M Yamashita Y (2005) Discrimination of Streptococcus pneumoniae from Viridans group streptococci by genomic subtractive hybridization. J Clin Microbiol 43 : 4528 4534.
24. Verhelst R, Kaijalainen T, De Baere T, Verschraegen G, Claeys G, Van Simaey L, De Ganck C Vaneechoutte M (2003) Comparison of five genotypic techniques for identification of optochin-resistant Pneumococcus-like isolates. J Clin Microbiol 41 : 3521 3525.
25. Walker NJ (2002) A technique whose time has come. Science 296 : 557 559.
26. Whatmore AM, Efstratiou A, Pickerill AP, Broughton K, Woodard G, Sturgeon D, George R Dowson CG (2000) Genetic relationships between clinical isolates of Streptococcus pneumoniae, Streptococcus oralis, and Streptococcus mitis: characterization of "Atypical" pneumococci and organisms allied to S. mitis harboring S. pneumoniae virulence factor-encoding genes. Infect Immun 68 : 1374 1382.
27. Yang C, Jiang Y, Huang K, Zhu C Yin Y (2003) Application of real-time PCR for quantitative detection of Campylobacter jejuni in poultry, milk and environmental water. FEMS Immunol Med Mic 38 : 265 271.
28. Yang S, Lin S, Khalil A, Gaydos C, Nuemberger E, Juan G, Hardick J, Bartlett JG, Auwaerter PG Rothman RE (2005) Quantitative PCR assay using sputum samples for rapid diagnosis of pneumococcal pneumonia in adult emergency department patients. J Clin Microbiol 43 : 3221 3226.