Extracellular complex of chitinolytic enzymes of Clostridium paraputrificum strain J4 separated by membrane ultrafiltration

Folia Microbiologica

Volumn 55, Issue 4, 2010, Pages 386-389

  Tishchenko, G ^a   Koppova I ^b   Simunek J ^b   Dohnalek J ^a  

^a INSTITUTE OF MACROMOLECULAR CHEMISTRY   (Czech Republic)

^b INSTITUTE OF ANIMAL PHYSIOLOGY AND GENETICS   (Czech Republic)

Author keywords

[No Author keywords available]

Indexed keywords

ACETYLGLUCOSAMINIDASE; BACTERIAL PROTEIN; CHITIN; CHITINASE;

ARTICLE; CHEMISTRY; CLOSTRIDIUM; ENZYME STABILITY; ENZYMOLOGY; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MOLECULAR WEIGHT; PH; TEMPERATURE; ULTRAFILTRATION; PROCEDURES;

ACETYLGLUCOSAMINIDASE; BACTERIAL PROTEINS; CHITIN; CHITINASE; CLOSTRIDIUM; ENZYME STABILITY; HUMANS; HYDROGEN-ION CONCENTRATION; MOLECULAR WEIGHT; TEMPERATURE; ULTRAFILTRATION;

CLOSTRIDIUM; CLOSTRIDIUM PARAPUTRIFICUM;

EID: 77955169239     PISSN: 00155632     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12223-010-0065-y     Document Type: Article

References (17)

1. Bidochka M. J., Tong K. I., Khachatourians G. G.: Partial purification and characterization of two extracellular N-acetyl-d-glucosaminidases produced by the entomopathogenic fungus Beauveria bassiana. Can. J. Microbiol. 39, 40-45 (1992).
2. Blum H., Beier H., Gross H. J.: Improved silver staining of plant proteins, RNA and DNA in polyacrylamide gels. Electrophoresis8, 93-99 (1987).
3. Bradford M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analyt. Biochem. 72, 248-254 (1976).
4. Cherkasov A. N.: Selective ultrafiltration. J. Membr. Sci. 50, 109-130 (1990).
5. Choi B. K., Kim K. Y., Yoo Y. J., Oh S. J., Choi J. H., Kim C. Y.: In vitro antimicrobial activity of a chitooligosaccharide mixture against Actinobacillus actinomycetemcomitans and Streptococcus mutans. Internat. J. Antimicrob. Agents18, 553-557 (2001).
6. Flint H. J., Zhang J. X., Martin J.: Multiplicity and expression of xylanases in the rumen cellulolytic bacterium Ruminococcus flavefaciens. Curr. Microbiol. 29, 139-143 (1994).
7. Huang R., Mendis E., Rajapakse N., Kim S.-K.: Strong electronic charge as an important factor for anticancer activity of chitooligosaccharides (COS). Life Sci. 78, 2399-2408 (2006).
8. Inglis P. V., Peberdy J. K.: Production and purification of chitinase from Ewingella americana, recently described pathogen of the mushroom Agaricus bisporus. FEMS Microbiol. Lett. 157, 189-194 (1997).
9. Koga D., Mitsutomi M., Kono M., Matsumiya M.: Biochemistry of chitinases. Experientia87(Suppl.), 111-123 (1999).
10. Kopečný J., Hodrová B., Stewart C. S.: The isolation and characterization of a rumen chitinolytic bacterium. Lett. Appl. Microbiol. 23, 195-198 (1996).
11. Laemmli U. K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature227, 680-685 (1970).
12. Liu B., Liu W. S., Han B. Q., Sun Y. Y.: Antidiabetic effects of chitooligosaccharides on pancreatic islet cells in streptozotocin-induced diabetic rats. World J. Gastroenterol. 13, 725-731 (2007).
13. Šimůnek J., Kopečný J., Hodrová B., Bartoňová H.: Identification and characterization of Clostridium paraputrificum, a chitinolytic bacterium of human digestive tract. Folia Microbiol. 47, 559-564 (2002).
14. Šimůnek J., Tishchenko G., Hodrová B., Bartoňová H.: Effect of chitosan on the growth of human colonic bacteria. Folia Microbiol. 51, 306-308 (2006).
15. Šimůnek J., Tishchenko G., Koppová I., Bartoňová H., Novotná Z.: Chitinolytic activities of Clostridium sp. JM2 isolated from stool of human administered perorally by chitosan. Folia Microbiol. 53, 249-254 (2008).
16. Tronsmo A., Harman G. E.: Detection and quantification of N-acetyl-β-d-glucosaminidase, chitobiosidase, and endochitinase in solutions and on gels. Analyt. Biochem. 208, 74-79 (1993).
17. Wang S. L., Chang W. T.: Purification and characterization of two bifunctional chitinases/lysozymes extracellulary produced by Pseudomonas aeruginosa K-187 in a shrimp and crab shell powder medium. Appl. Environ. Microbiol. 63, 380-386 (1997).