Improved multiplex reverse transcription-polymerase chain reaction to detect and identify five tospovirus species simultaneously

Journal of General Plant Pathology

Volumn 76, Issue 4, 2010, Pages 273-277

  Kuwabara, Katsuya ^a   Yokoi, Naoto ^b   Ohki, Takehiro ^c   Tsuda, Shinya ^c  

^a Gunma Agricultural Technology Center   (Japan)

^b Akita Agricultural Extension Office at Yamamoto Region   (Japan)

^c NATIONAL AGRICULTURAL RESEARCH CENTER   (Japan)

Author keywords

Capsicum chlorosis virus; Chrysanthemum stem necrosis virus; Degenerate primer; Diagnosis; Epidemiology; Tospovirus

Indexed keywords

ASTERACEAE; CAPSICUM CHLOROSIS VIRUS; CHRYSANTHEMUM; CHRYSANTHEMUM STEM NECROSIS VIRUS; IMPATIENS; IMPATIENS NECROTIC SPOT VIRUS; IRIS (ANGIOSPERM); IRIS YELLOW SPOT VIRUS; MAGNOLIOPHYTA; SOLANACEAE; TOMATO SPOTTED WILT VIRUS; TOSPOVIRUS;

EID: 77955097257     PISSN: 13452630     EISSN: 1610739X     Source Type: Journal    
DOI: 10.1007/s10327-010-0246-1     Document Type: Article

References (22)

1. Chu F-H, Chao C-H, Chung M-H, Chen C-C, Yeh S-D (2001) Completion of the genome sequence of Watermelon silver mottle virus and utilization of degenerate primers for detecting tospoviruses in five serogroups. Phytopathology 91: 361-368.
2. Doi M, Kato K (2002) New virus diseases in lisianthus caused by Iris yellow spot virus (IYSV) and Impatiens necrotic spot virus (INSV) (Abstract in Japanese). Jpn J Phytopathol 68: 231.
3. Doi M, Zen S, Okuda M, Nakamura H, Kato K, Hanada K (2003) Leaf necrosis disease of lisianthus (Eustoma grandiflorum) caused by Iris yellow spot virus. Jpn J Phytopathol 69: 181-188.
4. German TL, Ullman DE, Moyer JW (1992) Tospoviruses: diagnosis, molecular biology, phylogeny, and vector relationships. Annu Rev Phytopathol 30: 315-348.
5. Goto T, Sazarashi H, Nozawa H, Nakayama K, Natsuaki T (2001) Necrotic spot of cyclamen and exacum caused by Impatiens necrotic spot virus (INSV) (Abstract in Japanese). Jpn J Phytopathol 67: 173.
6. Hull R (2002) Genome organization. In: Hull R (ed) Matthews' plant virology, 4th edn. Academic Press, San Diego, pp 171-224.
7. Iwaki M, Honda Y, Hanada K, Tochihara H, Yonaha T, Hokama K, Yokoyama T (1984) Silver mottle disease of watermelon caused by Tomato spotted wilt virus. Plant Dis 68: 1006-1008.
8. Kato K, Hanada K, Kameya-Iwaki M (1999) Transmission mode, host range and electron microscopy of a pathogen causing a new disease of melon (Cucumis melo) in Japan. Ann Phytopathol Soc Jpn 65: 624-627.
9. Kawano T, Takahashi K, Takahashi Y, Tairako K, Tsukamoto S, Kobayashi Y, Honda Y (1999) Spotted wilt disease of verbena, Madagascar periwinkle and impatiens caused by Tomato spotted wilt virus (Abstract in Japanese). Ann Phytopathol Soc Jpn 65: 651.
10. Matsuura S, Hoshino S, Hayashi H, Kohguchi T, Hagiwara K, Omura T (2002) Effects of latent infection of stock plants and abundance of thrips on the occurrence of Tomato spotted wilt virus in chrysanthemum fields. J Gen Plant Pathol 68: 99-102.
11. Matsuura S, Kubota K, Okuda M (2007) First report of Chrysanthemum stem necrosis virus on chrysanthemums in Japan. Plant Dis 91: 468.
12. Mumford RA, Barker I, Wood KR (1996) The biology of the tospoviruses. Ann Appl Biol 128: 159-183.
13. Nichol ST, Beaty BJ, Elliott RM, Goldbach R, Plyusnin A, Schmalijohn CS, Tesh RB (2005) Genus Tospovirus. In: Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA (eds) Virus taxonomy. Eighth Report of the International Committee on the Taxonomy of Viruses. Elsevier, San Diego, pp 712-716.
14. Okuda M, Hanada K (2001) RT-PCR for detecting five distinct tospovirus species using degenerate primers and dsRNA template. J Virol Methods 96: 149-156.
15. Okuda M, Takeuchi S, Komi K, Iwanami T (2005) Occurrence of chlorosis on pepper (Capsicum annuum) caused by Capsicum chlorosis virus (Abstract in Japanese). Jpn J Phytopathol 71: 235.
16. Rozen S, Skaletsky HJ (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, pp 365-386.
17. Taba S, Naito T, Takaesu K (2000) Necrotic yellow disease of New Zealand spinach (Tetragonia expansa Murr.) caused by Watermelon silver mottle virus (WSMV) (Abstract in Japanese). Jpn J Phytopathol 66: 146-147.
18. Takeuchi S, Okuda M, Hanada K, Kawada Y, Kameya-Iwaki M (2001) Spotted wilt disease of cucumber (Cucumis sativus) caused by Melon yellow spot virus. Jpn J Phytopathol 67: 46-51.
19. Tanina K, Inoue K, Date H, Okuda M, Hanada K, Nasu H, Kasuyama S (2001) Necrotic spot disease of cineraria caused by Impatiens necrotic spot virus. Jpn J Phytopathol 67: 42-45.
20. Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22: 4673-4680.
21. Tsuda S, Hanada K, Fujisawa I, Kameya-Iwaki M, Tomaru K (1994) The demonstration of strain-specific antigenic determinants on nucleocapsid of Tomato spotted wilt virus by monoclonal antibodies. Ann Phytopathol Soc Jpn 60: 216-220.
22. Uga H, Tsuda S (2005) A one-step reverse transcription-polymerase chain reaction system for the simultaneous detection and identification of multiple tospovirus infections. Phytopathology 95: 166-171.