Development of a novel TaqMan real-time PCR assay for detecting rubella virus RNA

Journal of Virological Methods

Volumn 168, Issue 1-2, 2010, Pages 267-271

  Okamoto, Kiyoko ^a   Fujii, Kaoru ^a   Komase, Katsuhiro ^a  

^a NATIONAL INSTITUTE OF INFECTIOUS DISEASES   (Japan)

Author keywords

Diagnosis; RT PCR; Rubella; TaqMan

Indexed keywords

VIRUS RNA;

ARTICLE; CONTROLLED STUDY; DILUTION; GENE AMPLIFICATION; GENE EXPRESSION; GENOTYPE; HUMAN; NONHUMAN; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; RNA SEQUENCE; RUBELLA VIRUS; SALIVA ANALYSIS; SENSITIVITY AND SPECIFICITY; SEQUENCE ALIGNMENT; VIRUS DETECTION; VIRUS ISOLATION; VIRUS STRAIN; COMPARATIVE STUDY; EVALUATION STUDY; GENETICS; ISOLATION AND PURIFICATION; JAPAN; POLYMERASE CHAIN REACTION; PROCEDURES; RUBELLA; TIME; VIROLOGY;

RUBELLA; RUBELLA VIRUS;

HUMANS; JAPAN; POLYMERASE CHAIN REACTION; RNA, VIRAL; RUBELLA; RUBELLA VIRUS; SENSITIVITY AND SPECIFICITY; TIME FACTORS; VIROLOGY;

EID: 77955096729     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2010.05.016     Document Type: Article

References (19)

1. Abernathy E., Cabezas C., Sun H., Zheng Q., Chen M.H., Castillo-Solorzano C., Ortiz A.C., Osores F., Oliveira L., Whittembury A., Andrus J.K., Helfand R.F., Icenogle J. Confirmation of rubella within 4 days of rash onset: comparison of rubella virus RNA detection in oral fluid with immunoglobulin M detection in serum or oral fluid. J. Clin. Microbiol. 2009, 48:182-188.
2. CDC Global Measles and Rubella Laboratory Network, January 2004-June 2005. Morb. Mortal. Wkly. Rep. 2005, 54:1100-1104.
3. CDC Recommendations from an ad hoc Meeting of the WHO Measles and Rubella Laboratory Network (LabNet) on use of alternative diagnostic samples for measles and rubella surveillance. Morb. Mortal. Wkly. Rep. 2008, 57:657-660.
4. Cooray S., Warrener L., Jin L. Improved RT-PCR for diagnosis and epidemiological surveillance of rubella. J. Clin. Virol. 2006, 35:73-80.
5. Frey T.K. Molecular biology of rubella virus. Adv. Virus Res. 1994, 44:69-160.
6. Hübschen J.M., Kremer J.R., De Landtsheer S., Muller C.P. A multiplex TaqMan PCR assay for the detection of measles and rubella virus. J. Virol. Methods 2008, 149:246-250.
7. Jin L., Thomas B. Application of molecular and serological assays to case based investigations of rubella and congenital rubella syndrome. J. Med. Virol. 2007, 79:1017-1024.
8. Liang Y., Gillam S. Mutational analysis of the rubella virus nonstructural polyprotein and its cleavage products in virus replication and RNA synthesis. J. Virol. 2000, 74:5133-5141.
9. Oker-Blom C., Ulmanen I., Kääriäinen L., Pettersson R.F. Rubella virus 40S genome RNA specifies a 24S subgenomic mRNA that codes for a precursor to structural proteins. J. Virol. 1984, 49:403-408.
10. Pugachev K., Abernathy E., Frey T. Improvement of the specific infectivity of the rubella virus (RUB) infectious clone: determinants of cytopathogenicity induced by RUB map to the nonstructural proteins. J. Virol. 1997, 71:562-568.
11. Rajasundari T.A., Sundaresan P., Vijayalakshmi P., Brown D.W., Jin L. Laboratory confirmation of congenital rubella syndrome in infants: an eye hospital based investigation. J. Med. Virol. 2008, 80:536-546.
12. Shishido A., Ohtawara M. Development of attenuated rubella virus vaccines in Japan. Jpn. J. Med. Sci. Biol. 1976, 29:227-253.
13. Tipples G., Hamkar R., Mohktari-Azad T., Gray M., Ball J., Head C., Ratnam S. Evaluation of rubella IgM enzyme immunoassays. J. Clin. Virol. 2004, 30:233-238.
14. Vyse A., Jin L. An RT-PCR assay using oral fluid samples to detect rubella virus genome for epidemiological surveillance. Mol. Cell. Probes 2002, 16:93-97.
15. WHO Manual for the Laboratory Diagnosis of Measles Virus Infection 2007, World Health Organization, Department of Immunization, Vaccines and Biologicals, Geneva. Second edition.
16. WHO Update of standard nomenclature for wild-type rubella viruses, 2007. Wkly. Epidemiol. Rec. 2007, 82:216-222.
17. Yao J., Yang D., Chong P., Hwang D., Liang Y., Gillam S. Proteolytic processing of rubella virus nonstructural proteins. Virology 1998, 246:74-82.
18. Zhao L.H., Ma Y.Y., Wang H., Zhao S.P., Zhao W.M., Li H., Wang L.Y. Establishment and application of a TaqMan real-time quantitative reverse transcription-polymerase chain reaction assay for rubella virus RNA. Acta Biochim. Biophys. Sin. (Shanghai) 2006, 38:731-736.
19. Zhu Z., Xu W., Abernathy E., Chen M., Zheng Q., Wang T., Zhang Z., Li C., Wang C., He W., Zhou S., Icenogle J. Comparison of four methods using throat swabs to confirm rubella virus infection. J. Clin. Microbiol. 2007, 45:2847-2852.