Intranuclear detection of African swine fever virus DNA in several cell types from formalin-fixed and paraffin-embedded tissues using a new in situ hybridisation protocol

Journal of Virological Methods

Volumn 168, Issue 1-2, 2010, Pages 38-43

  Ballester, M ^a   Galindo Cardiel, I ^a   Gallardo, C ^b   Argilaguet, J M ^a   Segales J ^a,c   Rodriguez J M ^d,e   Rodriguez F ^a  

^a CENTRE DE RECERCA EN SANITAT ANIMAL CRESA   (Spain)

^b Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria   (Spain)

^c UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

^d UNIVERSIDAD AUTÓNOMA DE MADRID   (Spain)

^e INSTITUTO DE SALUD CARLOS III   (Spain)

Author keywords

ASFV; CD3; Digoxigenin; Formalin fixed, paraffin embedded tissues; ISH

Indexed keywords

DIGOXIGENIN; FORMALDEHYDE; PARAFFIN; FIXATIVE; VIRUS DNA;

AFRICAN SWINE FEVER VIRUS; ANIMAL TISSUE; ARTICLE; CELL LINEAGE; CELL NUCLEUS; CELL TYPE; CONTROLLED STUDY; ENDOTHELIUM CELL; IN SITU HYBRIDIZATION; LIVER CELL; LYMPHOCYTE; MACROPHAGE; MOLECULAR PROBE; MONOCYTE; NEUTROPHIL; NONHUMAN; PRIORITY JOURNAL; SENSITIVITY ANALYSIS; SIGNAL TRANSDUCTION; TARGET CELL; VIRUS DETECTION; VIRUS GENOME; AFRICAN SWINE FEVER; ANIMAL; EVALUATION STUDY; GENETICS; ISOLATION AND PURIFICATION; MOLECULAR PATHOLOGY; PROCEDURES; SWINE; VIROLOGY;

AFRICAN SWINE FEVER VIRUS;

AFRICAN SWINE FEVER; AFRICAN SWINE FEVER VIRUS; ANIMALS; CELL NUCLEUS; DNA, VIRAL; ENDOTHELIAL CELLS; FIXATIVES; FORMALDEHYDE; HEPATOCYTES; IN SITU HYBRIDIZATION; MACROPHAGES; MONOCYTES; NEUTROPHILS; PARAFFIN EMBEDDING; PATHOLOGY, MOLECULAR; SWINE; VIROLOGY;

EID: 77955096178     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2010.04.013     Document Type: Article

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