The novel OCT4 spliced variant OCT4B1 can generate three protein isoforms by alternative splicing into OCT4B

Journal of Genetics and Genomics

Volumn 37, Issue 7, 2010, Pages 461-465

  Gao, Yuan ^a,b   Wang, Xia ^a   Han, Jin ^a   Xiao, Zhifeng ^a   Chen, Bing ^a   Su, Guannan ^a   Dai, Jianwu ^a  

^a INSTITUTE OF GENETICS AND DEVELOPMENTAL BIOLOGY   (China)

^b UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

Author keywords

Alternative splicing; Alternative translation initiation; OCT4; Stress

Indexed keywords

DNA; MESSENGER RNA; OCTAMER TRANSCRIPTION FACTOR 4; OCTAMER TRANSCRIPTION FACTOR 4B; OCTAMER TRANSCRIPTION FACTOR 4B1; UNCLASSIFIED DRUG;

ALTERNATIVE RNA SPLICING; ANIMAL CELL; ARTICLE; CELL CULTURE; DNA TRANSFECTION; GENE MUTATION; INTRON; MOUSE; NONHUMAN; PROTEIN PROCESSING; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; WESTERN BLOTTING;

ALTERNATIVE SPLICING; CODON, TERMINATOR; EMBRYONIC STEM CELLS; EXONS; HUMANS; INTRONS; OCTAMER TRANSCRIPTION FACTOR-3; PROTEIN ISOFORMS; RNA SPLICING; RNA, MESSENGER; TRANSCRIPTION FACTORS;

EID: 77955064503     PISSN: 16738527     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1673-8527(09)60065-5     Document Type: Article

References (13)

1. Atlasi Y., Mowla S.J., Ziaee S.A., Gokhale P.J., Andrews P.W. OCT4 spliced variants are differentially expressed in human pluripotent and nonpluripotent cells. Stem Cells 2008, 26:3068-3074.
2. Cauffman G., Van de Velde H., Liebaers I., Van Steirteghem A. Oct-4 mRNA and protein expression during human preimplantation development. Mol. Hum. Reprod. 2005, 11:173-181.
3. Cauffman G., Liebaers I., Van Steirteghem A., Van de Velde H. POU5F1 isoforms show different expression patterns in human embryonic stem cells and preimplantation embryos. Stem Cells 2006, 24:2685-2691.
4. Ho S.N., Hunt H.D., Horton R.M., Pullen J.K., Pease L.R. Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene 1989, 77:51-59.
5. Irimia M., Penny D., Roy S.W. Coevolution of genomic intron number and splice sites. Trends Genet. 2007, 23:321-325.
6. Keller E.B., Noon W.A. Intron splicing: a conserved internal signal in introns of animal pre-mRNAs. Proc. Natl. Acad. Sci. USA 1984, 81:7417-7420.
7. Keller E.B., Noon W.A. Intron splicing: a conserved internal signal in introns of Drosophila pre-mRNAs. Nucleic Acids Res. 1985, 13:4971-4981.
8. Kotoula V., Papamichos S.I., Lambropoulos A.F. Revisiting OCT4 expression in peripheral blood mononuclear cells. Stem Cells 2008, 26:290-291.
9. Papamichos S.I., Kotoula V., Tarlatzis B.C., Agorastos T., Papazisis K., Lambropoulos A.F. OCT4B1 isoform: the novel OCT4 alternative spliced variant as a putative marker of stemness. Mol. Hum. Reprod. 2009, 15:269-270.
10. Scholer H.R., Ruppert S., Suzuki N., Chowdhury K., Gruss P. New type of POU domain in germ line-specific protein Oct-4. Nature 1990, 344:435-439.
11. Takeda J., Seino S., Bell G.I. Human Oct3 gene family: cDNA sequences, alternative splicing, gene organization, chromosomal location, and expression at low levels in adult tissues. Nucleic Acids Res. 1992, 20:4613-4620.
12. Wang X., Dai J. Isoforms of OCT4 contribute to the confusing diversity in stem cell biology. Stem Cells 2010, 28:885-893.
13. Wang X., Zhao Y., Xiao Z., Chen B., Wei Z., Wang B., Zhang J., Han J., Gao Y., Li L., Zhao H., Zhao W., Lin H., Dai J. Alternative translation of OCT4 by an internal ribosome entry site and its novel function in stress response. Stem Cells 2009, 27:1265-1275.